Specific accumulation and growth inhibitory effects of hybrid liposomes to hepatoma cells In vitro

Nakano, Koji; Iwamoto, Yasunori; Takata, Wataru; Matsumoto, Yōko; Ueoka, Ryuichi

doi:10.1016/s0960-894x(02)00723-0

Cited by 35 publications

(14 citation statements)

References 11 publications

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“…25 We are now establishing a novel approach to cancer chemotherapy using hybrid liposomes composed of 90 mol% DMPC and 10 mol% C 12 (EO) 10 . It is noteworthy that prolonged survival of patients with lymphoma and a remarkable reduction of solid tumor has been achieved by using hybrid liposomes without drugs, after the treatment was approved by the committee of bioethics.…”

Section: Resultsmentioning

confidence: 99%

Novel mechanism of hybrid liposomes‐induced apoptosis in human tumor cells

Matsumoto

Iwamoto

Matsushita

et al. 2005

Intl Journal of Cancer

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Hybrid liposomes can be prepared by simply ultrasonicating a mixture of vesicular and micellar molecules in a buffer solution. The physical properties of these liposomes, such as size, membrane fluidity, phase transition temperature and hydrophobicity can be controlled by changing the composition. Hybrid liposomes composed of dimyristoylphosphatidylcholine and polyoxyethylene (10) dodecyl ether were found to inhibit the growth of human promyelocytic leukemia (HL-60) cells without using any drugs. Induction of apoptosis by hybrid liposomes in HL-60 cells was verified on the basis of fluorescence microscopy and flow cytometry analysis, after fusion and accumulation of hybrid liposomes, which was revealed on the basis of microphysiometer. We elucidated the pathways of apoptosis induced by the hybrid liposomes. That is, hybrid liposomes fused and accumulated in tumor cell membranes, and the apoptosis signal first passed through mitochondria, caspase-9 and caspase-3, second through Fas, caspase-8, caspase-3 and then reached the nucleus. Hybrid liposomes themselves can induce apoptosis in human tumor cells along with high inhibitory effects on the growth of tumor cells. ' 2005 Wiley-Liss, Inc.

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Section: Resultsmentioning

confidence: 99%

Novel mechanism of hybrid liposomes‐induced apoptosis in human tumor cells

Matsumoto

Iwamoto

Matsushita

et al. 2005

Intl Journal of Cancer

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“…HL-n potently inhibited the growth of these cancer cells at IC 50 How do HL-n inhibit the growth of cancer cells? We have already reported that hybrid liposomes composed of DMPC and polyoxyethylene(n) alkyl ethers induced apoptosis in cancer cells such as liver cancer, 30 lung cancer, 22 breast cancer, 25 colon cancer, 28 lymphoma, 27 and primary effusion lymphoma. 31 In the present study, we confirmed the induction of apoptosis by HL-n in cancer cells on the basis of the TUNEL method using confocal laser microscopy.…”

Section: Acs Medicinal Chemistry Lettersmentioning

confidence: 99%

Membrane-Targeted Nanotherapy with Hybrid Liposomes for Tumor Cells Leading to Apoptosis

Komizu

Nakata

Goto

et al. 2011

ACS Med. Chem. Lett.

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Hybrid liposomes are nanosized liposomal particles and can be prepared by sonication of vesicular and micellar molecules in a buffer solution. In this study, we obtained the first successful experiment resulting in a good correlation between inhibitory effects of hybrid liposomes on the growth of various tumor cells and the membrane fluidity of tumor cells (plasma membranes). The results indicated that hybrid liposomes could provide the possibility of novel membrane-targeted nanotherapy for intractable cancers.

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“…The physical properties of HL such as shape, size, membrane fluidity of phase transition can be controlled by changing the constituents and compositional ratio. Remarkably high inhibitory effects of HL composed of DMPC and polyoxyethylene(n) dodecyl ethers (C 12 (EO) n ) on the growth of tumor cells in vitro [13][14][15][16][17], in vivo [17][18][19][20][21], and clinical application [22][23][24] have been obtained without drugs. We have reported HL induced apoptosis in various cancer cells including NSCLC (A549, H460, H23 and H520) cells [25].…”

Section: Introductionmentioning

confidence: 99%

Inhibitory Effects of Hybrid Liposomes on the Growth of Non-small Cell Lung Carcinoma Cells and Anti-invasive Activity by Ceramide Generation without any Drugs

Komizu¹,

Matsumoto²

2015

J Carcinog Mutagen

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Ceramide generation and invasive inhibition by hybrid liposomes (HL) composed of 90 mol% L-α-dimyristoylphosphatidylcholine (DMPC) and 10 mol% polyoxyethylene (23) dodecyl ether (C 12 (EO) 23 ) were examined for human lung cancer (A549) cells in vitro. Ceramide generation for A549 cells treated with HL was observed through the activation of neutral sphingomyelinase. These was a reduction in sphingomyelin in A549 cells after treatment with HL. The anti-invasive effects of HL for A549 cells were obtained through the inhibition of MT1MMP/MMP14. Inhibitory effects of HL on the migration of A549 cells was obtained using scratch assay.

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Specific accumulation and growth inhibitory effects of hybrid liposomes to hepatoma cells In vitro

Cited by 35 publications

References 11 publications

Novel mechanism of hybrid liposomes‐induced apoptosis in human tumor cells

Novel mechanism of hybrid liposomes‐induced apoptosis in human tumor cells

Membrane-Targeted Nanotherapy with Hybrid Liposomes for Tumor Cells Leading to Apoptosis

Inhibitory Effects of Hybrid Liposomes on the Growth of Non-small Cell Lung Carcinoma Cells and Anti-invasive Activity by Ceramide Generation without any Drugs

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