Membrane-Targeted Nanotherapy with Hybrid Liposomes for Tumor Cells Leading to Apoptosis

Abstract

Hybrid liposomes are nanosized liposomal particles and can be prepared by sonication of vesicular and micellar molecules in a buffer solution. In this study, we obtained the first successful experiment resulting in a good correlation between inhibitory effects of hybrid liposomes on the growth of various tumor cells and the membrane fluidity of tumor cells (plasma membranes). The results indicated that hybrid liposomes could provide the possibility of novel membrane-targeted nanotherapy for intractable cancers.

Hybrid Nanoparticles Inhibit Growth of Human Prostate Cancer Cells by Induction of Apoptosis

“…On the other hand, HL composed of DMPC and polyoxyethylene(n)dodecyl ethers (C 12 (EO) n ) without any drugs have remarkable inhibitory effects on the growth of various tumor cells including leukemia, lymphoma and colorectal cancer along with apoptosis in vitro [7], in vivo [8,9] and clinical applications [10,11].In this study, we examined the inhibitory effects of hybrid liposomes (HL) composed of DMPC and C 12 (EO) 25 on the growth of human prostate cancer cells in vitro.Hybrid liposomes (HL) are nanosized liposomal particles (Figure 1 A) and can be prepared by sonication of a mixture containing 90 mol% DMPC (NOF, Japan) and 10 mol% C 12 (EO) n (n = 25: Nikko Chemicals, Japan) in 5% glucose solution as described previously [12]. The liposomes composed of DMPC alone (DMPC liposomes) were prepared in the same manner as described above.…”

“…The mean d hy of HL was about 33 nm in diameter with a single and narrow distribution and was stable for 35 days, though d hy of DMPC liposomes gradually increased with time. It is worthy to note that HL having size under 100 nm in diameter could avoid the reticular endothelial system in vivo [13] and thus should be appropriate for the intravenous administration in vivo and clinical applications.First, we examined the 50% inhibitory concentration (IC 50 ) of HL on the growth of human prostate (DU145 and PC-3) cancer cells with WST-8 assay [12][13][14]. The cells (5.0 × 10 4 viable cells/ ml) were seeded into 96 well plates and incubated for 48 hrs in humidified 5% CO 2 at 37°C in the presence or absence of HL.…”

“…First, we examined the 50% inhibitory concentration (IC 50 ) of HL on the growth of human prostate (DU145 and PC-3) cancer cells with WST-8 assay [12][13][14]. The cells (5.0 × 10 4 viable cells/ ml) were seeded into 96 well plates and incubated for 48 hrs in humidified 5% CO 2 at 37°C in the presence or absence of HL.…”

“…In this study, we examined the inhibitory effects of hybrid liposomes (HL) composed of DMPC and C 12 (EO) 25 on the growth of human prostate cancer cells in vitro.…”

Novel Liposomes Composed of Dimyristoylphosphatidylcholine and Trehalose Surfactants Inhibit the Growth of Tumor Cells along with Apoptosis

“…9,10) The mechanistic details of apoptosis for tumor cells induced by HL 7) and the correlation between antitumor effects and membrane fluidity of HL 11) and membrane fluidity of plasma membranes of tumor cells 12) have been clarified. Successful clinical chemotherapy with drug-free HL to patients with lymphoma has been reported without having any adverse effects after the approval of the Bioethics Committee.…”