Hybrid liposomes inhibit tumor growth and lung metastasis of murine osteosarcoma cells

Abstract

Antitumor effects of hybrid liposomes (HL) composed of l-α-dimyristoylphosphatidylcholine (DMPC) and polyoxyethylene(23) dodecyl ether (C12(EO)23) on the metastatic growth of murine osteosarcoma (LM8) cells were investigated in vitro and in vivo. Remarkable inhibitory effects of HL-23 on the growth of LM8 cells were obtained through the induction of apoptotic cell death in vitro. It was also indicated that HL-23 should dramatically suppress the invasion of LM8 cells and the formation of filopodia on the cell surface in vitro. Furthermore, significantly high therapeutic effects were observed in the homograft mouse models of LM8 cells with lung metastasis after the treatment with HL-23 in vivo. That is, the histological analysis demonstrated that the primary tumor growth of LM8 cells implanted subcutaneously into the mice was inhibited along with the induction of apoptosis. In addition, it was found that HL-23 significantly decreased the lung metastasis of LM8 cells in the mouse models through the inhibition of primary tumor invasion. These results suggest that HL-23 could be a novel agent for the chemotherapy of osteosarcoma.

Cell Cycle Arrest by Hybrid Liposomes for Human Lung Carcinoma Cells

“…Phosphorylated-Akt (p-Akt) kinases regulate the cell-cycle progress via down-regulation of p21 and p27 [19]. Akt signaling is also implicated in apoptotic mitochondrial pathways via upregulation of pro-apoptotic proteins and down-regulation of antiapoptotic proteins [19,20]. Therefore, we examined the inhibition of the expression of p-Akt in A549 cells by HL.…”

“…To assess the filopodia formation of A549 cells, the cells were analyzed by total internal reflection fluorescence (TIRF) microscopy [20]. The cells (2.0 × 10 4 cells/mL) were seeded in glass bottom dishes (Mat Tek, Flint, MI, USA) and incubated for 24 hours.…”

Liposomes Aid Curcumin's Combat with Cancer in a Breast Tumor Model

“…The benefits of using a phospholipid bilayer nanoconstruct, as shown in Figures 1 (A), 1 (B), and 1 (C), include dual loading of hydrophilic and hydrophobic drugs, improved bioavailability to target site cell and tissue, stability of encapsulated drugs, pharmacological inactivity and minimal toxicity of phospholipids [29,40]. Several investigations have shown the benefits of encapsulating curcumin in different cancer types such as pancreatic adenocarcinoma, osteosarcoma, liver cancer etc [41][42][43][44]. Different types of lipid based nanosystems, such as long circulating PEGylated, cationic liposomes and so on, have shown promise due to the flexibility of altering its properties by changing the composition and concentration of lipids [41,45,46].…”

“…Kitajima et. al. demonstrated the anticancer activity of liposomal formulations of curcumin in vivo against osteosarcoma in mice [42]. Nanoemulsions encapsulating curcumin have also been explored for activity against cancer [43].…”

Inhibitory Effects of Hybrid Liposomes on the Growth of Non-small Cell Lung Carcinoma Cells and Anti-invasive Activity by Ceramide Generation without any Drugs

“…The physical properties of HL such as shape, size, membrane fluidity of phase transition can be controlled by changing the constituents and compositional ratio. Remarkably high inhibitory effects of HL composed of DMPC and polyoxyethylene(n) dodecyl ethers (C 12 (EO) n ) on the growth of tumor cells in vitro [13][14][15][16][17], in vivo [17][18][19][20][21], and clinical application [22][23][24] have been obtained without drugs. We have reported HL induced apoptosis in various cancer cells including NSCLC (A549, H460, H23 and H520) cells [25].…”