Specific Accumulation and Antitumor Effects of Hybrid Liposomes on the Growth of Lung Tumor Cells

Abstract

In general, chemotherapeutic eŠects were low for non-small cell lung cancer (NSCLC) in the lung tumor. We examined the accumulation and antitumor eŠects of hybrid liposomes (HL-23) composed of phospholipid (L-adimyristoylphosphatidylcholine: DMPC) and PEG surfactant [polyoxyethylene (23)

Inhibitory Effects of Hybrid Liposomes on the Growth of Non-small Cell Lung Carcinoma Cells and Anti-invasive Activity by Ceramide Generation without any Drugs

“…The invasion rate of A549 cells treated with HL significantly decreased in a dose-dependent manner (Figures 4A and 4B). Interestingly, the anti-invasive effects of HL were observed at low concentrations (100, 150 and 200 μM) without growth-inhibition under IC 50 values (238 μM) [25]. …”

“…Hybrid liposomes (HL) were prepared by sonication of a mixture containing 90 mol% L-α-dimyristoylphosphatidylcholine (DMPC, NOF, Tokyo, Japan) and 10 mol% polyoxyethylene(23) dodecylether (C 12 (EO) 25 , Nikko Chemicals, Tokyo, Japan,) in 5% glucose solution using bath type sonicater (VS-N300, VELVO-CLEAR, Tokyo, Japan) at 45°C with 300 W. The sample solutions were filtered using a membrane filter (Advantec, Tokyo, Japan) with 0.20 μm pore size and stored at room temperature (25°C). The DMPC liposomes were prepared in the same manner as described above.…”

“…Remarkably high inhibitory effects of HL composed of DMPC and polyoxyethylene(n) dodecyl ethers (C 12 (EO) n ) on the growth of tumor cells in vitro [13][14][15][16][17], in vivo [17][18][19][20][21], and clinical application [22][23][24] have been obtained without drugs. We have reported HL induced apoptosis in various cancer cells including NSCLC (A549, H460, H23 and H520) cells [25].…”

Membrane fluidity matters: Hyperthermia from the aspects of lipids and membranes

“…Several solid tumours are characterised by the higher fluidity of their cell membranes [16][17][18][19][20], correlating with their proliferative and invasive potentials and their metastatic abilities [21][22][23]. A reversal of tumour resistance to apoptotic stimuli through the alteration of membrane fluidity was suggested by Baritaki et al [12].…”

Membrane-Targeted Nanotherapy with Hybrid Liposomes for Tumor Cells Leading to Apoptosis

“…[1][2][3][4][5] Similar findings were observed for other cancer cell membranes. [6][7][8][9][10] The membrane dynamics with higher fluidity should be a fundamental property of cancer cells and closely related to the biological features of the proliferative potential, invasive potential, and metastatic ability. [11][12][13][14][15] On the other hand, conventional chemotherapy for cancers often has severe side effects that limit their efficacy, as most current anticancer drugs are directed against a nonspecific target DNA or designed to interfere with nucleic acid biosynthesis.…”