Species-wide Metabolic Interaction Network for Understanding Natural Lignocellulose Digestion in Termite Gut Microbiota

Kundu, Pritam; Manna, Bharat; Majumder, Subham; Ghosh, Amit

doi:10.1038/s41598-019-52843-w

Cited by 32 publications

(20 citation statements)

References 57 publications

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“…The metagenomic analysis tools can also be applied during the biologically mediated substrate preparation steps prior to the actual fermentation. For instance, Kundu et al [24] classified the metabolic interaction network of gut compartments of Nasutitermes corniger used for lignin disintegration. The pretreatment of lignin-rich materials is critical for biomass utilization in the bioethanol production process [25].…”

Section: Biofuelmentioning

confidence: 99%

RETRACTED ARTICLE: Metagenomics for taxonomy profiling: tools and approaches

et al. 2020

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The study of metagenomics is an emerging field that identifies the total genetic materials in an organism along with the set of all genetic materials like deoxyribonucleic acid and ribose nucleic acid, which play a key role with the maintenance of cellular functions. The best part of this technology is that it gives more flexibility to environmental microbiologists to instantly pioneer the immense genetic variability of microbial communities. However, it is intensively complex to identify the suitable sequencing measures of any specific gene that can exclusively indicate the involvement of microbial metagenomes and be able to advance valuable results about these communities. This review provides an overview of the metagenomic advancement that has been advantageous for aggregation of more knowledge about specific genes, microbial communities and its metabolic pathways. More specific drawbacks of metagenomes technology mainly depend on sequence-based analysis. Therefore, this 'targeted based metagenomics' approach will give comprehensive knowledge about the ecological, evolutionary and functional sequence of significantly important genes that naturally exist in living beings either human, animal and microorganisms from distinctive ecosystems.

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Section: Biofuelmentioning

confidence: 99%

RETRACTED ARTICLE: Metagenomics for taxonomy profiling: tools and approaches

et al. 2020

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“…It is possible to contrive a species-wide metabolic interaction network of the termite gut-microbiome in order to have a system-level understanding of metabolic communication. Kundu et al (2019) have elucidated 15 crucial hemicellulolytic microbes and their corresponding enzyme machinery ( Kundu et al, 2019 ). At present, no insect has been found to be able to completely digest lignocellulose food via cellulase and hemicellulase secreted by itself ( Sun & Chen, 2010 ).…”

Section: Introductionmentioning

confidence: 99%

Diversity of the gut microbiome in three grasshopper species using 16S rRNA and determination of cellulose digestibility

Wang¹,

Bai²,

Zheng³

et al. 2020

PeerJ

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Background Grasshoppers are typical phytophagous pests, and they have large appetites with high utilization of plants fibers, the digestion of which may depend on the microorganisms in their intestines. Grasshoppers have the potential to be utilized in bioreactors, which could improve straw utilization efficiency in the future. In this study, we describe the gut microbiome in three species of grasshoppers, Oedaleus decorus asiaticus, Aiolopus tamulus and Shirakiacris shirakii, by constructing a 16S rDNA gene library and analyzed the digestibility of cellulose and hemicellulose in the grasshoppers by using moss black phenol colorimetry and anthrone colorimetry. Results There were 509,436 bacterial OTUs (Operational Taxonomic Units) detected in the guts of all the grasshoppers sampled. Among them, Proteobacteria and Firmicutes were the most common, Aiolopus tamulus had the highest bacterial diversity, and Shirakiacris shirakii had the highest bacterial species richness. The intestinal microflora structure varied between the different species of grasshopper, with Aiolopus tamulus and Shirakiacris shirakii being the most similar. Meanwhile, the time at which grasshopper specimens were collected also led to changes in the intestinal microflora structure in the same species of grasshoppers. Klebsiella may form the core elements of the microflora in the grasshopper intestinal tract. The digestibility of cellulose/hemicellulose among the three species grasshoppers varied (38.01/24.99%, 43.95/17.21% and 44.12/47.62%). LEfSe analysis and Spearman correlation coefficients showed that the hemicellulosic digestibility of Shirakiacris shirakii was significantly higher than that of the other two species of grasshopper, which may be related to the presence of Pseudomonas, Stenotrophomonas, Glutamicibacter, Corynebacterium, and Brachybacterium in Shirakiacris shirakii intestinal tract. Conclusion The intestinal microbial communities of the three grasshoppers species are similar on phylum level, but the dominant genera of different species grasshoppers are different. The cellulose digestibility of the three species of grasshoppers is relatively high, which may be correlated with the presence of some gut microbiome. Increasing the understanding of the structure and function of the grasshopper intestinal microflora will facilitate further research and the utilization of intestinal microorganisms in the future.

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“…Another interesting example of the use of shotgun metagenomics to understand microbial degradation of plant cell wall polymers concerns the recent work by Kundu et al (2019). The authors firstly obtained sequences from public shotgun metagenomics datasets of DNA extracted from different gut compartments of the termite Nasutitermes corniger.…”

Section: Metagenomicsmentioning

confidence: 99%