Species specific marker peptides for meat authenticity assessment: A multispecies quantitative approach applied to Bolognese sauce

Prandi, Barbara; Varani, M.; Faccini, Andrea; Lambertini, Francesca; Suman, Michele; Leporati, Andrea; Tedeschi, Tullia; Sforza, Stefano

doi:10.1016/j.foodcont.2018.10.016

Cited by 55 publications

(59 citation statements)

References 25 publications

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“…Samples were centrifuged at 12,000 rpm for 10 min at 4°C and the clear supernatant was used for the analysis. Peptides were separated by reverse phase liquid chromatography and identified using both high and low resolution tandem mass spectrometry, as described in Prandi et al [33]. The relative quantification of the peptides was obtained by direct comparison of the peak areas and of the -10lgP values, which are program scores indicating the quality of the identification.…”

Section: Methodsmentioning

confidence: 99%

Enzymatic production of bioactive peptides from scotta, an exhausted by-product of ricotta cheese processing

et al. 2019

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The present work reports the enzymatic valorisation of the protein fraction of scotta, a dairy by-product representing the exhausted liquid residue of ricotta production. Scotta was subjected to ultra-filtration with membrane cut-offs from 500 to 4 kDa and the obtained protein-enriched fractions were used for the optimization of enzyme-based digestions aimed at producing potentially bioactive peptides. Nine different commercial proteases were tested and the best digestion conditions were selected based on protein yield, fraction bioactivity and foreseen scale up processing costs. Scale up of the 3% Pancreatin or 5% Papain processes was performed up to 2 L (37°C or 60°C respectively, 1 h incubation), and the digestion efficiency increased with the reaction volume as well as antioxidant activity (up to 60 gBSA eq/L and to 1.7 gAA eq/L). Retentate 1 digested fractions also showed, for the first time in dairy-based peptides, anti-tyrosinase activity, up to 0.14 gKA eq/L. Digested proteins were sub-fractionated by means of physical membrane separations and 30–10 kDa fraction from Papain treatment showed the highest antioxidant and anti-tyrosinase activities. The peptide sequence of the most bioactive fractions was achieved.

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Section: Methodsmentioning

confidence: 99%

Enzymatic production of bioactive peptides from scotta, an exhausted by-product of ricotta cheese processing

et al. 2019

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“…Low-resolution mass spectrometry (LRMS) analysis was performed on digested cocoa samples, in order to identify the amino acid sequences of the peptides. LRMS analysis was performed in positive mode accordingly to Prandi et al [23]. Prior to LC-MS analysis, samples were centrifuged at 6708× g , 4 °C for 10 min to precipitate insoluble compounds.…”

Section: Methodsmentioning

confidence: 99%

Simulated Gastrointestinal Digestion of Cocoa: Detection of Resistant Peptides and In Silico/In Vitro Prediction of Their Ace Inhibitory Activity

et al. 2019

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In this study we investigated the oligopeptide pattern in fermented cocoa beans and derived products after simulated gastrointestinal digestion. Peptides in digested cocoa samples were identified based on the mass fragmentation and on the software analysis of vicilin and 21 KDa cocoa seed protein sequences, the most abundant cocoa proteins. Quantification was carried out by liquid chromatography/electrospray ionisation mass spectrometry (LC/ESI-MS) using an internal standard. Sixty five peptides were identified in the digested samples, including three pyroglutamyl derivatives. The in vitro angiotensin-converting enzyme (ACE)-inhibitory activity of cocoa digests were tested, demonstrating a high inhibition activity, especially for digestates of cocoa beans. The peptides identified were screened for their potential ACE inhibitory activity through an in silico approach, and about 20 di-, three- and tetra-peptides actually present in our samples were predicted as active. Two of the potentially active peptides were chemically synthesized and then assessed for their inhibitory activity by using the ACE in vitro assay. These peptides demonstrated an ACE inhibitory activity, however, that was too weak to explain alone the high activity of cocoa digestates, suggesting a synergic effect of all cocoa peptides. As a whole, results showed that an average chocolate portion (30 g) ensures an amount of peptides after digestion that, assuming complete absorption, could reach almost a complete inhibition of ACE.

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“…The 2013 horsemeat crisis, whilst being an infringement of consumer rights, and causing supply chain disruption leading to a loss of sales and widespread product recalls did not show any profiles of public health risks (Premanandh, 2013;Czinkota, Kaufmann & Basile, 2014). In response to the horsemeat crisis, the EU Food Fraud Network (EU FFN) was set up in 2013 and the Administrative Assistance and Cooperation System (AAC) was made available for Member States in 2015 (Prandi et al 2019). Since then, the EU FFN & AAC System and the RASFF System have been working together in synergy to maintain the EU safety and compositional standards for food and feed (EC, 2016).…”

Section: Study Approachmentioning

confidence: 99%

Food supplements’ non-conformity in Europe – Poland: A case study

Kowalska

Bieniek

Manning

2019

Trends in Food Science & Technology

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Background: Mislabelling and substitution of ingredients in food supplements is a growing concern for regulators, businesses and consumers. Whilst there is a body of literature that has considered food and drink substitution and mislabelling, there is limited published research on the compliance of food supplements with regulatory requirements. Scope and Approach: Using secondary data, the aim of this research was to identify the main factors influencing food supplements non-compliance in the European Union (EU) but with specific emphasis on Poland. The sources of data in this review were: (1) the register of prohealth foods maintained by the Chief Sanitary Inspector (GIS) in Poland; (2) unpublished data from the European Commission DG Health and Food Safety (EC DG SANTE); (3) the EU Food Fraud Network and the Administrative Assistance and Cooperation System (EU FFN & AAC) Reports; (4) the Polish Trade Inspection (IH) Report; and (5) the Rapid Alert System for Food and Feed (RASFF) Portal. Key findings and conclusions: The level of food supplements non-compliance with stated legal requirements especially mislabelling is identified in this research. Policy needs to be strengthened both at the EU level, where overarching regulatory governance can be introduced, and also in individual member states, such as Poland, where situational socioeconomic factors Highlights  The incidence of food supplement non-conformity is a concern in Europe.  Problems involve composition, nutrition, and health claims.  Effective food supplement governance is essential for consumer protection.

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Species specific marker peptides for meat authenticity assessment: A multispecies quantitative approach applied to Bolognese sauce

Cited by 55 publications

References 25 publications

Enzymatic production of bioactive peptides from scotta, an exhausted by-product of ricotta cheese processing

Enzymatic production of bioactive peptides from scotta, an exhausted by-product of ricotta cheese processing

Simulated Gastrointestinal Digestion of Cocoa: Detection of Resistant Peptides and In Silico/In Vitro Prediction of Their Ace Inhibitory Activity

Food supplements’ non-conformity in Europe – Poland: A case study

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