Species or Genotypes? Reassessment of Four Recently Described Species of the Ceratocystis Wilt Pathogen, <i>Ceratocystis fimbriata</i>, on <i>Mangifera indica</i>

Oliveira, Leonardo S. S.; Harrington, T. C.; Ferreira, M. A.; Damacena, Michelle B.; Al-Sadi, Abdullah M.; Al-Mahmooli, Issa Hashil; Alfenas, Acelino C.

doi:10.1094/phyto-03-15-0065-r

Cited by 68 publications

(97 citation statements)

References 46 publications

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“…Thus, all of the isolates investigated were confirmed to represent the single species C. manginecans. When species concepts alternative to the genealogical concordance phylogenetic concept are considered, these isolates could be seen as different haplotypes of C. fimbriata (Oliveira et al, 2015). However, based on previous phylogenetic analyses including five gene regions (Fourie et al, 2014) we consider C. manginecans as a species distinct from the sweet potato black rot fungus which includes the type of C.…”

Section: Discussionmentioning

confidence: 99%

A possible centre of diversity in South East Asia for the tree pathogen, Ceratocystis manginecans

Fourie

Wingfield

et al. 2016

Infection, Genetics and Evolution

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Highlights• In Oman and Pakistan, C. manginecans from native legumes are genetically identical to the clonal population from M. indica.• The data suggest a possible area of origin for C. manginecans in South East Asia, particularly Vietnam.• Microsatellite primers were designed, from the genome of C. manginecans, that can be used in a multiplex PCR approach. ABSTRACTThe fungal pathogen, Ceratocystis manginecans, has caused serious canker and wilt disease on Mangifera indica (mango), on legume tree species in Oman and Pakistan and on manginecans. Unlike the populations in Oman and Pakistan, relatively high levels of genetic variation were found for the isolates from Indonesia and Vietnam. The Vietnam population was significantly differentiated from the other populations and isolates from this area had the highest level of genetic diversity thus far encountered for the pathogen.

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Section: Discussionmentioning

confidence: 99%

A possible centre of diversity in South East Asia for the tree pathogen, Ceratocystis manginecans

Fourie

Wingfield

et al. 2016

Infection, Genetics and Evolution

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“…(). Sequences of the nuclear internal transcribed spacers ITS1‐5.8S‐ITS2 (ITS) or translation elongation factor 1‐alpha (TEF1‐ α ) were amplified using primers ITS1F/ITS4 or ERCF1.5/ERCF6 separately (White et al ., ; Oliveira et al ., ). Sequences of TEF1‐ α were specific to Ambrosiella (Mayers et al ., ; Lin et al ., ).…”

Section: Methodsmentioning

confidence: 99%

Plasticity of mycangia in Xylosandrus ambrosia beetles

Ruan

Stanley

et al. 2018

Insect Science

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Insects that depend on microbial mutualists evolved a variety of organs to transport the microsymbionts while dispersing. The ontogeny and variability of such organs is rarely studied, and the microsymbiont's effects on the animal tissue development remain unknown in most cases. Ambrosia beetles (Coleoptera: Curculionidae: Scolytinae or Platypodinae) and their mutualistic fungi are an ideal system to study the animal-fungus interactions. While the interspecific diversity of their fungus transport organ-mycangia-is well-known, their developmental plasticity has been poorly described. To determine the ontogeny of the mycangium and the influence of the symbiotic fungus on the tissue development, we dissected by hand or scanned with micro-CT the mycangia in various developmental stages in five Xylosandrus ambrosia beetle species that possess a large, mesonotal mycangium: Xylosandrus amputatus, Xylosandrus compactus, Xylosandrus crassiusculus, Xylosandrus discolor, and Xylosandrus germanus. We processed 181 beetle samples from the United States and China. All five species displayed three stages of the mycangium development: (1) young teneral adults had an empty, deflated and cryptic mycangium without fungal mass; (2) in fully mature adults during dispersal, the pro-mesonotal membrane was inflated, and most individuals developed a mycangium mostly filled with the symbiont, though size and symmetry varied; and (3) after successful establishment of their new galleries, most females discharged the bulk of the fungal inoculum and deflated the mycangium. Experimental aposymbiotic individuals demonstrated that the pronotal membrane invaginated independently of the presence of the fungus, but the fungus was required for inflation. Mycangia are more dynamic than previously thought, and their morphological changes correspond to the phases of the symbiosis. Importantly, studies of the fungal symbionts or plant pathogen transmission in ambrosia beetles need to consider which developmental stage to sample. We provide illustrations of the different stages, including microphotography of dissections and micro-CT scans.

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“…The most aggressive strains on a particular host are most likely to be sampled because they cause the most crop damage. In addition, vegetative propagation of a host such as taro or pruning a host such as mango could increase the proportion of aggressive genotypes in populations (Ferreira et al ., ; Harrington et al ., ; Oliveira et al ., ).…”

Section: Discussionmentioning

confidence: 97%

“…(). These microsatellite markers were mapped onto the C. fimbriata genome (Simpson et al ., ) and used in previous population studies on C. cacaofunesta (Engelbrecht et al ., ), C. platani (Engelbrecht et al ., ; Ocasio‐Morales et al ., ), C. pirilliforms (Nkuekam et al ., ), and C. fimbriata (van Wyk et al ., ; Ferreira et al ., , , ; Harrington et al ., ; Oliveira et al ., ; Li et al ., ). Of the 16 loci used in some of the earlier studies, two (CfCAT3K and CfCAT9X) were not used because their alleles could not be consistently resolved with some isolates.…”

Section: Methodsmentioning

confidence: 99%

Populations of Ceratocystis fimbriata on Colocasia esculenta and other hosts in the Mata Atlântica region in Brazil

et al. 2017

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Ceratocystis fimbriata is native to Brazil, where it is able to cause serious diseases on numerous hosts, especially on non-native plants. Because C. fimbriata is soilborne and not wind dispersed, highly differentiated populations are found in different regions of Brazil. The present study compared populations of C. fimbriata on taro, mango, eucalyptus and kiwifruit from the coastal Mata Atlântica region with native populations of the fungus from the Cerrado-transition region in Brazil by using 14 SSR markers and DNA sequences of ITS and mating type genes. Microsatellite and phylogenetic analyses were performed to test the hypothesis that populations on different hosts from the Mata Atlântica region are related to each other and are native to the region. The ITS sequences varied greatly among the taro isolates, with six sequences identified, from which two had not been previously reported. For mating type genes, four sequences were identified among the isolates on taro, mango, eucalyptus and kiwifruit. Phylogenetic analyses showed that Mata Atlântica populations formed a monophyletic group distinct from Cerrado-transition region populations, although earlier studies had shown that isolates from the two regions are interfertile and are considered as a single biological species. Microsatellite analysis revealed low gene diversity for each of the three Mata Atlântica populations on taro, mango and kiwifruit, suggesting that these populations had gone through genetic bottlenecks, probably by dispersal of select genotypes in vegetative propagation material. Also, microsatellite markers showed that two microsatellite genotypes from taro are widely spread in Brazil, probably by infected corms.

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Species or Genotypes? Reassessment of Four Recently Described Species of the Ceratocystis Wilt Pathogen, Ceratocystis fimbriata, on Mangifera indica

Cited by 68 publications

References 46 publications

A possible centre of diversity in South East Asia for the tree pathogen, Ceratocystis manginecans

A possible centre of diversity in South East Asia for the tree pathogen, Ceratocystis manginecans

Plasticity of mycangia in Xylosandrus ambrosia beetles

Populations of Ceratocystis fimbriata on Colocasia esculenta and other hosts in the Mata Atlântica region in Brazil

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