Species identification of blood and bloodstains by high-performance liquid chromatography

Inouel, Hiroyuki; Takabe, Fukutaro; Takenaka, Osamu; Iwasa, Mitsuhiro; Maeno, Yoshitaka

doi:10.1007/bf01816476

Cited by 35 publications

(15 citation statements)

References 14 publications

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“…Several HPLC methods have been developed, which could simultaneously identify a substance, such as blood and determine its origin of species. Fresh blood and blood stains from human and animal species were analyzed using this method by Inoue et al 11 The sensitivity of this approach can be improved using fluorescence detection with ultraviolet excitation. Mass spectrometry (MS) was demonstrated as an effective tool in identifying species by Espinoza et al 12 They use the properties of hemoglobin in blood samples as a means of differentiating one species from another.…”

Section: Introductionmentioning

confidence: 99%

Discrimination of human and nonhuman blood using Raman spectroscopy with self-reference algorithm

Bian

Wang

et al. 2017

J. Biomed. Opt

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We report a self-reference algorithm to discriminate human and nonhuman blood by calculating the ratios of identification Raman peaks to reference Raman peaks and choosing appropriate threshold values. The influence of using different reference peaks and identification peaks was analyzed in detail. The Raman peak at 1003 cm-1 was proved to be a stable reference peak to avoid the influencing factors, such as the incident laser intensity and the amount of sample. The Raman peak at 1341 cm-1 was found to be an efficient identification peak, which indicates that the difference between human and nonhuman blood results from the C-H bend in tryptophan. The comparison between self-reference algorithm and partial least square method was made. It was found that the self-reference algorithm not only obtained the discrimination results with the same accuracy, but also provided information on the difference of chemical composition. In addition, the performance of self-reference algorithm whose true positive rate is 100% is significant for customs inspection to avoid genetic disclosure and forensic science.

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Section: Introductionmentioning

confidence: 99%

Discrimination of human and nonhuman blood using Raman spectroscopy with self-reference algorithm

Bian

Wang

et al. 2017

J. Biomed. Opt

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“…This may also result from the extra peaks and unsteady baselines, as mentioned above. These extra peaks and distortions of the chromatographic profiles have also been observed in animal samples [17]. The ratios of the a-chain area to the heme area on the chromatograms from animal bloodstains might also decrease in the same manner.…”

Section: Discussionmentioning

confidence: 89%

Identification of fetal hemoglobin and simultaneous estimation of bloodstain age by high-performance liquid chromatography

et al. 1991

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A method using reverse-phase high-performance liquid chromatography (HPLC) for the identification of fetal hemoglobin (Hb F) and the simultaneous estimation of bloodstain age is described. Umbilical cord and neonatal bloodstains can be differentiated from adult stains by the presence of gamma-globin chains which are characteristic of Hb F. With this method, cord and neonatal blood could be distinguished from adult blood in stains up to 32 weeks old. The age of the stain was estimated from the ratio of the peak area of the alpha-globin chain to that of heme on the same chromatogram. The ratio decreased gradually with an increase in the age of the stain up to 20 weeks old. Studies performed at each time period revealed no significant difference in the ratios of cord and neonatal bloodstains or in the ratios of cord and adult stains. The regression equation calculated from the ratios (y) and the ages of stains in weeks (x) expressed logarithmically is y = 2.5758-0.2497 In (x) and the coefficient of correlation is -0.7491 (n = 252, P less than 0.001). The present method, having the advantages of simplicity, speed and sensitivity, should be of great value to forensic science.

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“…Tabela 2 -Métodos de identificação de sangue humano por ELISA. É possível também fazer a diferenciação de amostras de sangue de diferentes espécies por cromatografia a líquido de alta eficiência (CLAE) pela separação das cadeias de globina (INOUE et al, 1990;ANDRASKO;ROSÉN, 1994), porém essa técnica exige um equipamento de custo elevado sendo, portanto, de acesso restrito aos laboratórios forenses.…”

Section: Pesquisa De Sangue Humanounclassified

Identificação e tipagem de sangue humano em amostras forenses por Enzima I

Franck¹,

Albuquerque²

2012

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A identificação de sangue humano em amostras forenses é um pré-requisito importante aos exames de DNA que possibilitam o estabelecimento de relações entre indivíduos ou entre indivíduos e objetos envolvidos em infrações penais. Este trabalho teve como objetivo uma revisão bibliográfica dos métodos empregados para a confirmação de sangue humano, bem como, para a tipagem ABO e determinação de outros marcadores por ELISA (ensaio de imunoabsorção ligado à enzima) em amostras de importância forense. Métodos imunoenzimáticos são específicos, rápidos, sensíveis e permitem a automatização das análises, sendo, portanto, adequados à rotina de laboratórios forenses.

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Species identification of blood and bloodstains by high-performance liquid chromatography

Cited by 35 publications

References 14 publications

Discrimination of human and nonhuman blood using Raman spectroscopy with self-reference algorithm

Discrimination of human and nonhuman blood using Raman spectroscopy with self-reference algorithm

Identification of fetal hemoglobin and simultaneous estimation of bloodstain age by high-performance liquid chromatography

Identificação e tipagem de sangue humano em amostras forenses por Enzima I

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