Species identification in fish fillet products using DNA barcoding

Pinto, Angela Di; Marchetti, Patrizia; Mottola, Anna; Bozzo, Giancarlo; Bonerba, Elisabetta; Ceci, Edmondo; Bottaro, Marilisa; Tantillo, Giuseppina

doi:10.1016/j.fishres.2015.05.006

Cited by 71 publications

(54 citation statements)

References 27 publications

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“…Furthermore, our biodiversity studies help tourism sector too, because a natural environment rich in biodiversity, as well as taking environmental importance, become a real tourist attraction, providing cultural and esthetic benefits (see Madonna et al 2015). A practical use of barcoding approach updates the database for new species, their discrimination for correct labeling (Di Pinto et al 2015), to avoid substitution of specimens with less economic value, poisoning, and the onset of allergies or just to select properly the species with the highest quantity of antioxidants (Guerriero et al 2008). This species update can contribute to the necessity of periodic checks on the communities' diversity and catalog (Lahaye et al 2008;Crocetta et al 2015) and in particular may become a potential management tool for local administrations to protect Gaeta Gulf and Alexandria Harbor habitats (Scheffers et al 2012;Madonna et al 2015) and the health of ecosystems (Lahaye et al 2008; http://www.rac-spa.org/ sites/default/files/doc_cop/biodiversity.pdf).…”

Section: Discussionmentioning

confidence: 99%

Fisheries and biodiversity along Mediterranean Sea: Italian and Egyptian coast overview

Guerriero

Rabbito

Alwany

et al. 2017

Euro-Mediterr J Environ Integr

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Mediterranean fish species living along Italian (Gaeta) and Egyptian (Alexandria) coasts were analyzed using DNA barcodes for molecular identification. Mitochondrial Cytochrome c Oxidase subunit 1 (COI) gene was sequenced from 31 different marine species to test whether the morphology-based assignment of individuals into 19 families, 6 orders was supported by DNA-based species delimitation and Neighbour Joining cladogram. All COI rRNA gene barcodes were matched with reference sequences of expected species, according to morphological identification. Neighbour joining tree was drawn based on COI rRNA gene and the majority of specimens clustered in agreement with their taxonomic classification. Our results updated Mediterranean edible fish knowledge providing graphical resources, taxonomical and bioinformatics references, improving the genetic fish database and the basic molecular information to strengthen the science-policy interface for biodiversity and ecosystem services as conservation, blue economy, and long-term human well-being.

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Section: Discussionmentioning

confidence: 99%

Fisheries and biodiversity along Mediterranean Sea: Italian and Egyptian coast overview

Guerriero

Rabbito

Alwany

et al. 2017

Euro-Mediterr J Environ Integr

View full text Add to dashboard Cite

show abstract

“…Accurate species identification is also relevant to customers wishing to make informed choices to avoid CFP high risk species. However, studies suggest that internationally, comprehensive information about fish products, such as the commercial name, scientific name, and geographical source, are often not available [298], and that 25%–40% of seafood in commercial channels may be mislabeled by species [299,300,301,302,303,304]. In addition, international seafood supply chains are generally longer and more complex than those of locally or domestically traded fish.…”

Section: International Trade Tourism and Traceabilitymentioning

confidence: 99%

“…DNA barcodes [156,157] and Radio Frequency Identification Device (RFID) tags have been used for authentication and tracking of seafood in commerce [298,305,313,340,341,342]. However, there is a need for rapid handheld instruments that can be used by fishers, wholesalers, and retailers.…”

Section: Future Directionsmentioning

confidence: 99%

An Updated Review of Ciguatera Fish Poisoning: Clinical, Epidemiological, Environmental, and Public Health Management

et al. 2017

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Ciguatera Fish Poisoning (CFP) is the most frequently reported seafood-toxin illness in the world. It causes substantial human health, social, and economic impacts. The illness produces a complex array of gastrointestinal, neurological and neuropsychological, and cardiovascular symptoms, which may last days, weeks, or months. This paper is a general review of CFP including the human health effects of exposure to ciguatoxins (CTXs), diagnosis, human pathophysiology of CFP, treatment, detection of CTXs in fish, epidemiology of the illness, global dimensions, prevention, future directions, and recommendations for clinicians and patients. It updates and expands upon the previous review of CFP published by Friedman et al. (2008) and addresses new insights and relevant emerging global themes such as climate and environmental change, international market issues, and socioeconomic impacts of CFP. It also provides a proposed universal case definition for CFP designed to account for the variability in symptom presentation across different geographic regions. Information that is important but unchanged since the previous review has been reiterated. This article is intended for a broad audience, including resource and fishery managers, commercial and recreational fishers, public health officials, medical professionals, and other interested parties.

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“…In spite of such difference, authors highlighted that functional attributes as efficient products recall, possibility to identify an individual responsibility and a complete traceability of the meat chain may be regarded as minimum requirements of any authentication system for all consumers [35,56]. Keeping all these aspects in mind, the potential of DNA technologies appears straightforward, as recently well documented for fishery products [57][58][59].…”

Section: Resultsmentioning

confidence: 99%

DNA Barcoding as a Molecular Tool to Track Down Mislabeling and Food Piracy

2015

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DNA barcoding is a molecular technology that allows the identification of any biological species by amplifying, sequencing and querying the information from genic and/or intergenic standardized target regions belonging to the extranuclear genomes. Although these sequences represent a small fraction of the total DNA of a cell, both chloroplast and mitochondrial barcodes chosen for identifying plant and animal species, respectively, have shown sufficient nucleotide diversity to assess the taxonomic identity of the vast majority of organisms used in agriculture. Consequently, cpDNA and mtDNA barcoding protocols are being used more and more in the food industry and food supply chains for food labeling, not only to support food safety but also to uncover food piracy in freshly commercialized and technologically processed products. Since the extranuclear genomes are present in many copies within each cell, this technology is being more easily exploited to recover information even in degraded samples or transformed materials deriving from crop varieties and livestock species. The strong standardization that characterizes protocols used worldwide for DNA barcoding makes this technology particularly suitable for routine analyses required by agencies to safeguard food safety and quality. Here we conduct a critical review of the potentials of DNA barcoding for food labeling along with the main findings in the area of food piracy, with particular reference to agrifood and livestock foodstuffs.

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Species identification in fish fillet products using DNA barcoding

Cited by 71 publications

References 27 publications

Fisheries and biodiversity along Mediterranean Sea: Italian and Egyptian coast overview

Fisheries and biodiversity along Mediterranean Sea: Italian and Egyptian coast overview

An Updated Review of Ciguatera Fish Poisoning: Clinical, Epidemiological, Environmental, and Public Health Management

DNA Barcoding as a Molecular Tool to Track Down Mislabeling and Food Piracy

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