Species identification and population structure analysis in<i>Geranium</i>subg.<i>Geranium</i>(Geraniaceae)

Esfandani‐Bozchaloyi, Somayeh; Sheidai, Masoud; Keshavarzi, Maryam; Noormohammadi, Zahra

doi:10.1515/hacq-2017-0007

Cited by 33 publications

(32 citation statements)

References 46 publications

(41 reference statements)

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“…Geography appears to play an important role in isolation by distance, particularly for Mediterranean plants. Reductions in gene flow may lead to the appearance of new species or subspecies, with isolation in glacial refugia as a major promoter of such diversification (ESFANDANI-BOZCHALOYI et al, 2018a;2018b;2018c;2018d). E. cicutarium is of wide spread in our country and it has several medicinal applications (WIESNEROVA and WIESNER, 2004), however we had no information on its genetic structure and detailed taxonomic information.…”

Section: Discussionmentioning

confidence: 99%

Population differentiation and gene flow in Rrodium cicutarium: A potential medicinal plant

2020

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Erodium (Geranaiceae) species are distributed in different habitats of Iran. Some species are of medicinal importance while some are well known weeds. In arid and semi-arid regions, E. cicutarium has had some importance as a forage plant and is an important grazing plant and source of protein supplements to straw for ruminants in semi deserts and wastelands of the Middle East. There is no information on its population genetic structure, genetic diversity, and morphological variability in Iran. Due to the medicinal importance of this species, a genetic variability and populations? structure study is performed studying 15 geographical populations of E. cicutarium Therefore, we used six inter-retrotransposon amplified polymorphism (IRAP) markers and 15 combined IRAP markers to reveal within and among population genetic diversity in this plant. AMOVA test produced significant genetic difference (PhiPT = 0.39, P = 0.010) among the studied populations and also revealed that, 55% of total genetic variability was due to within population diversity while, 45% was due to among population genetic differentiation. Mantel test showed positive significant correlation between genetic distance and geographical distance of the studied populations. Networking, STRUCTURE analyses and population assignment test revealed some degree of gene flow among these populations. PCoA plot of populations based on morphological characters was in agreement with MDS plot of molecular data. These results indicated that geographical populations of E. cicutarium are well differentiated both in genetic content as well as morphological characteristics. Consensus tree based on morphological and genetic data separated some of these populations from the others suggesting the existence of ecotypes within this species.

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Section: Discussionmentioning

confidence: 99%

Population differentiation and gene flow in Rrodium cicutarium: A potential medicinal plant

2020

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“…RAPD analysis using PCR along with short arbitrary sequence primers has been reported sensitive to detecting variation at level of individuals. The benefits of this method are: a) a large number of samples are tested easily and efficiently using less quantity of material; b) the DNA amplicons are independent of ontogenetic expression; c) several genomic regions may be sampled with likely infinite numbers of markers (SONIYA et al 2001;ESFANDANI-BOZCHALOYI et al, 2018 a;2018b;2018c;2018d).…”

Section: Introductionmentioning

confidence: 99%

RAPD profiling in detecting genetic variation in Stellaria L. (Caryophyllaceae)

2021

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Stellaria species are common herbs, preferred humid mountainously slopes, but some grew in desert. Main center of diversification for Stellaria is Eurasia, with a center of distribution in the mountains of central Asia. Some species are also cosmopolitan. It is represented by 9 species in Iran. The genus has high medicinal value. To determine the genetic diversity and understand the species? limits within the Iranian Stellaria, we produced molecular data using 139 randomly collected plants representing 8 species from five provinces of Iran. A total of 122 reproducible bands were generated by 10 of 25 random amplified polymorphic DNA (RAPD) primers, with an average of 12.2 bands/primer and 33% polymorphism. Largest number of effective alleles (Ne), genetic diversity (H), and Shannon Index (I) were shown by S. media. Our data depicted highest similarity between S. media and S. pallida and lowest between S. media and S. graminea. S. pallida showed relatively low level of genetic variation. Finally, the Neighbor Joining (NJ) trees based on RAPD markers data divided the populations into two different clusters, indicating their genetic difference which is discussed in details.

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“…Similarly, the pollen morphological features are significantly useful at the generic level (Perveen & Gaiser, ), while the stem and leaf anatomy are useful to differentiate the sections (Salimpour, ). ISSR molecular markers were shown to be informative for genetic diversity and population structure studies (Esfandani‐ Bozchaloyi et al, , , , , , , , ). There has been no detailed macro and micro‐morphological study on Geranium species in Iran.…”

Section: Introductionmentioning

confidence: 99%

Taxonomic significance of macro and micro‐morphology of Geranium L. species Using Scanning Electron Microscopy

Esfandani‐Bozchaloyi

Zaman

2018

Microscopy Res & Technique

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Macro and micromorphology of different parts that is, sepals, petals, flowers, indumentum, and leaf shape of 22 Geranium L. taxa from Iran were studied. These taxa representing the eight sections of the genus as sect. Dissecta, sect. Geranium, and sect. Tuberosa (of subgen. Geranium); sect. Batrachioidea, sect. Divaricata, sect. Lucida, sect. Ruberta and sect. Trilopha (of subgen.Robertium), that were collected from different geographical habitats of Iran. The biometric study involved 27 quantitative and 33 qualitative characters which were studied by use of stereomicroscopy and scanning electron microscopy (SEM). Statistical analyses were done by use of PAST software. Principal Component Analysis defined the most variable characters. Results showed that the diagnostic features were; length, width, apex of sepals, petals, trichomes type in pedicles and sepals along with their density, the shape length and width of leaves. In general, the present study revealed that the species could be differentiated by macro and micromorphological characters. Taxa of two subgenera were clearly separated based on selected characters. These characters were found useful for the taxonomic identification and species delimitation in almost all taxa studied at the sub generic level, although some species of section Geranium and section Dissecta are mixed and nested in both subgenea.

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Species identification and population structure analysis inGeraniumsubg.Geranium(Geraniaceae)

Cited by 33 publications

References 46 publications

Population differentiation and gene flow in Rrodium cicutarium: A potential medicinal plant

Population differentiation and gene flow in Rrodium cicutarium: A potential medicinal plant

RAPD profiling in detecting genetic variation in Stellaria L. (Caryophyllaceae)

Taxonomic significance of macro and micro‐morphology of Geranium L. species Using Scanning Electron Microscopy

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