Species Identification and Antimicrobial Resistance of Coagulase‐Negative Staphylococci Isolated from the Meat of Sea Fish

Coagulase-negative staphylococci (CNS) are ubiquitous micro-organisms that are commonly present on animal skin and animal-derived foods. They are members of the beneficial microbial consortia of several fermented food products where they contribute to quality. Currently, only a few CNS species are included in commercial starter cultures, although many other ones with promising properties have been isolated from diverse food ecosystems. In the present study, a Strengths-Weaknesses-Opportunities-Threats (SWOT) analysis of the potential use of unconventional CNS starter cultures for the fermentation of animal-derived foods is carried out. An overview of both their desirable and worrisome metabolic traits is given. In general, the application of innovative CNS-based starter cultures offers opportunities to modulate flavour, improve the safety and health aspects and develop novel colour development strategies for clean label products. Yet, their implementation is often not straightforward as nontrivial obstacles or threats are encountered, which relate to technological, food safety and legal concerns. As most of the desirable and undesirable characteristics of CNS species are strain dependent, a case-by-case evaluation is needed when evaluating specific strains for their potential use as novel starter cultures.

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“…) and fish (Regecová et al . ). As a result, CNS species are present in fermented meats (Aquilanti et al .…”

Section: Introductionmentioning

confidence: 97%

Nonconventional starter cultures of coagulase-negative staphylococci to produce animal-derived fermented foods, a SWOT analysis

2018

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“…Matrix‐Assisted Laser Desorption Ionization‐Time of Flight Mass Spectrometry (MALDI‐TOF MS) has been successfully implemented for the identification of different bacterial genera of relevance in aquaculture (Regecova et al., ). Several studies have also shown that MALDI‐TOF MS could presumably efficiently identify Aeromonas species (Benagli et al., ; Chen et al., ; Deng et al., ; Donohue, Smallwood, Pfaller, Rodgers & Shoemaker, ; Donohue et al., ; Lamy, Kodjo, Laurent & Col, ; Rychert et al., ).…”

Section: Introductionmentioning

confidence: 99%

Limited performance of MALDI‐TOF for identification of fish Aeromonas isolates at species level

Cerda

Fernández-Bravo

Domı́nguez³

et al. 2018

Journal of Fish Diseases

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The aim of this study was to evaluate the usefulness of the MALDI-TOF MS to identify 151 isolates of Aeromonas obtained mostly from diseased fish. MALDI-TOF MS correctly identified all isolates to the genus level but important differences in the percentage of isolates correctly identified depending on the species were observed. Considering exclusively the first identification option, Aeromonas bestiarum, Aeromonas hydrophila, Aeromonas salmonicida, Aeromonas veronii and Aeromonas sobria were the best identified with results >95%. However, considering the first and second identification options, the only species that showed values >90% was A. hydrophila. Overall, when the database was supplemented with 14 new spectra, the number of accurate identifications increased (41% vs. 55%) and the number of inconclusive identifications decreased (45% vs. 29%), but great differences in the success of species-level identifications were found. Species-distinctive mass peaks were identified only for A. hydrophila and A. bestiarum (5003 and 7360 m/z in 95.5% and 94.1% of their isolates, respectively). This work demonstrates the utility of MALDI-TOF MS for Aeromonas identification to the genus level, but there is no consistency for the accurate identification of some of the most prevalent species implicated in fish disease.

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“…To distinguish between pathogenic CoPS and nonpathogenic coagulase-negative species, clumping factor, DNase and hyaluronidase are of great importance. However, some of those factors were also found in clinical and food isolates of CoNS [48,[50][51][52][53]. Results of our study demonstrate the absence of coagulase, DNase and hyaluronidase production, with lecithinase activity confirmed in 38.6% and gelatinase activity confirmed in 49.1% of CoNS isolates.…”

Section: Discussionmentioning

confidence: 55%

Virulence factors and antimicrobial resistance of coagulase-negative staphylococci isolated from drinking water

et al. 2015

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Little has been published about the occurrence, speciesidentification, andpathogenicpotentialofcoagulasenegative staphylococci (CoNS) present in drinking water. In this study, ten species were identified among 57 isolates of staphylococci from 756 samples of chlorinated drinking water taken from public distribution networks in the Slovak Republic. S. warneri (37%), S. haemolyticus (23%), and S. saprophyticus ssp. saprophyticus (14%) were identified most frequently. Isolates did not produce coagulase, DNase, or hyaluronidase; production of gelatinase and lecithinase was observed in 28 and 22 isolates, respectively. Genetically encoded ability for production of enterotoxin SED was revealed in two isolates. Among ten antibiotics tested, resistance to ampicillin (66.7%), penicillin (64.9%), and erythromycin (57.9%) were observed most frequently. Resistance to gentamicin, vancomycin, or clindamycin was not confirmed. Production of β-lactamase was observed in 64.9% of isolates. Fourty-two isolates were resistant to two or more antibiotics tested, and eight isolates showed multiresistance. The presence of mecA gene was confirmed in 8 isolates, while PBP2a was revealed in 7. Two isolates of S. epidermidis were identified as methicillin-resistant (MRSE). The results demonstrate that CoNS in chlorinated drinking water may possess virulence factors and show resistance to various antibiotics. Therefore, their pathogenic potential should not be ignored.

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Species Identification and Antimicrobial Resistance of Coagulase‐Negative Staphylococci Isolated from the Meat of Sea Fish

Cited by 21 publications

References 38 publications

Nonconventional starter cultures of coagulase-negative staphylococci to produce animal-derived fermented foods, a SWOT analysis

Nonconventional starter cultures of coagulase-negative staphylococci to produce animal-derived fermented foods, a SWOT analysis

Limited performance of MALDI‐TOF for identification of fish Aeromonas isolates at species level

Virulence factors and antimicrobial resistance of coagulase-negative staphylococci isolated from drinking water

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