The Piper genus is a member of the piperaceae family which is spread throughout the tropics and subtropics from the lowlands to the highlands, including Indonesia. Piper is an important spice and phytopharmaca plant that is used by the Indonesian people for their needs for spices and traditional medicine. This research was conducted with the aim of knowing the morphological variations of plants belonging to the genus Piper found in Banyumas, Purbalingga, and Banjarnegara regencies. A total of 30 individuals belonging to the genus Piper were observed using 39 morphological characters. The method used is a survey method with a purposive random sampling technique. The data obtained from morphological observations were analyzed descriptively to obtain morphological variations of members of the genus Piper. The results of research in Banyumas, Purbalingga and Banjarnegara regencies found 6 plants belonging to the Piper genus, namely red betel (Piper crocatum Ruiz & Pav.), green betel (Piper betle L.), yellow betel (Piper sp.), pepper (Piper nigrum L. .), cubeb (Piper cubeba L.f.), Javanese chili (Piper retrofractum Vahl). Variations in morphological characters of 6 plants belonging to the genus Piper were found in plant height, stem color, distance between internodes on the stem, leaf blade shape, leaf blade tip, leaf base. leaf color, color of the upper surface of the leaf, color of the lower surface of the leaf, length of the leaf blade, width of the leaf blade, texture of the upper and lower surface of the leaf, texture of the petiole, length of the petiole, color of the petiole, and aroma on the leaves.