Species discrimination of Radix Bupleuri through the simultaneous determination of ten saikosaponins by high performance liquid chromatography with evaporative light scattering detection and electrospray ionization mass spectrometry

Lee, Jae-Hyun; Yang, Dong-Hyug; Suh, Joon Hyuk; Kim, Unyong; Eom, Han Young; Kim, Jung-Hyun; Lee, Mi Young; Kim, Jinwoong; Han, Sang Beom

doi:10.1016/j.jchromb.2011.10.040

Cited by 21 publications

(12 citation statements)

References 25 publications

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“…Pure SB3, SB4, and SD were prepared as described previously with some modifications (Lee et al, 2011). Briefly, dried roots of BF (14.16 kg) were sliced, extracted with methanol (MeOH), and evaporated under reduced pressure.…”

Section: Sample Preparationsmentioning

confidence: 99%

Ethanol extract of Bupleurum falcatum and saikosaponins inhibit neuroinflammation via inhibition of NF-κB

Park

Kang

Piao

et al. 2015

Journal of Ethnopharmacology

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Section: Sample Preparationsmentioning

confidence: 99%

Ethanol extract of Bupleurum falcatum and saikosaponins inhibit neuroinflammation via inhibition of NF-κB

Park

Kang

Piao

et al. 2015

Journal of Ethnopharmacology

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“…Saikosaponins (SSs) are triterpene saponins isolated from the root of Bupleurum falcatum L. (Umbelliferae) (1). As all SSs have a common steroid-like structure, they were expected and also confirmed to exert a number of steroid-associated pharmacological activities (2,3).…”

Section: Introductionmentioning

confidence: 99%

“…SSs are also regarded as the major effective components of xiao-chai-hu-tang, one of the most popular Chinese medicinal formulae that has been widely used for its various pharmacological effects, including anti-inflammatory, antioxidant and antihepatic fibrosis properties (4-6). Thus far, at least 10 types of SS have been identified, and among which saikosaponin-d (SSd) is considered to be the most active component (1,7). Studies have reported that SS may potently inhibit the proliferation of hepatocellular carcinoma (8), cervical cancer (9), lung adenocarcinoma (10), colon carcinoma (11), breast cancer (12) and melanoma (13) cells.…”

Section: Introductionmentioning

confidence: 99%

Saikosaponin-d inhibits proliferation of DU145 human prostate cancer cells by inducing apoptosis and arresting the cell cycle at G0/G1 phase

Yao

Yang

Cao

et al. 2014

Molecular Medicine Reports

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Saikosaponin‑d (SSd), a triterpene saponin compound derived from Bupleurum radix, has been shown to have a cytotoxic effect on various cancer cell lines. However, its effect on prostate cancer cells has remained unexplored. The present study reports the apoptosis‑inducing effect of SSd on the DU145 human prostate carcinoma cell line. Treatment with SSd inhibited DU145 cell proliferation in a concentration‑dependent manner. Flow cytometric analysis showed that SSd inhibited the proliferation of DU145 cells by induction of apoptosis and cell cycle arrest at G0/G1 phase. Further mechanistic experiments demonstrated that SSd arrested the cell cycle at G0/G1 phase via upregulation of p53 and p21 and induced apoptosis by modulating B‑cell lymphoma 2 family proteins, dissipation of the mitochondrial membrane potential, release of cytochrome c into the cytosol and activation of caspase‑3. In conclusion the present study indicated that SSd induced apoptosis in DU145 cells by the intrinsic apoptotic pathway. Therefore, SSd may become a leading candidate drug for the therapy of prostate carcinoma.

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“…Several studies have established a fast resolution liquid chromatography coupled with ELSD method and successfully separated multi-samples in RB from different source (Huang et al, 2009a). Based on these investigations, in 2010, a rapid and improved HPLC-ELSD method was also established to evaluate the quality of SSs, which confirmed three major constituents SSa, SSc and SSd and simultaneously detected ten different SSs in RB extract (Lee et al, 2011b). A recent study further optimized one of the countercurrent separation techniques, centrifugal partition chromatography, coupled with HPLC-ELSD method to efficiently obtain purified SSa and SSc from modified two-phase solvent system-derived SSs enriched RB extract (Yoon and Kim, 2009).…”

Section: Extraction and Identification Of Sssmentioning

confidence: 99%

A comprehensive review and perspectives on pharmacology and toxicology of saikosaponins

Huang

et al. 2018

Phytomedicine

138

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Background: Radix Bupleuri (RB) has been widely used in Chinese Traditional Medicine for over 2000 years and is currently marketed in China as Chai-Hu-Shu-Gan tablets and Xiao-Yao-Wan tablets. Saikosaponins (SSs, especially SSa, SSc and SSd), as the major bioactive compounds in RB, represent anti-inflammatory, anti-tumor, anti-oxidant, anti-viral and hepatoprotective effects. Purpose: To summarize recent findings regarding to the extraction, detection, biosynthesis, metabolism, pharmacological/toxicological effects of SSs. Methods: Online academic databases (including PubMed, Google Scholar, Web of Science and CNKI) were searched using search terms of "Saikosaponin", "Radix Bupleuri", "Bupleurum" and combinations to include published studies of SSs primarily from 2003 to 2018. Several critical previous studies beyond this period were also included. Results: 354 papers were found and 165 papers were reviewed. SSs have drawn great attention for their antiinflammation, anti-viral and anti-cancer effects and contradictory roles in the regulation of cell apoptosis, oxidative stress and liver fibrosis. Meanwhile, increased risks of overdose-induced acute or accumulation-related chronic hepatotoxicity of SSs and RB have also been reported. However, underlying mechanisms of SSs bioactivities, the metabolism of SSs and bioactivities of SSs metabolites are largely unknown. Conclusion: This comprehensive review of SSs provides novel insights and perspectives on the limitations of current studies and the importance of metabolism study and the dose-pharmacological/toxic relationship of SSs for the future discovery of SSs-based therapeutic strategies and clinical safe practice.

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Species discrimination of Radix Bupleuri through the simultaneous determination of ten saikosaponins by high performance liquid chromatography with evaporative light scattering detection and electrospray ionization mass spectrometry

Cited by 21 publications

References 25 publications

Ethanol extract of Bupleurum falcatum and saikosaponins inhibit neuroinflammation via inhibition of NF-κB

Ethanol extract of Bupleurum falcatum and saikosaponins inhibit neuroinflammation via inhibition of NF-κB

Saikosaponin-d inhibits proliferation of DU145 human prostate cancer cells by inducing apoptosis and arresting the cell cycle at G0/G1 phase

A comprehensive review and perspectives on pharmacology and toxicology of saikosaponins

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