Saikosaponin-d inhibits proliferation of DU145 human prostate cancer cells by inducing apoptosis and arresting the cell cycle at G0/G1 phase

Yao, Min; Yang, Jingbo; Cao, Lanqing; Qu, S.; Gao, Hongwen

doi:10.3892/mmr.2014.2153

Cited by 43 publications

(27 citation statements)

References 40 publications

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“… 2014a, 2014b ; Yao et al. 2014 ), activates caspases apoptosis pathway, including the activation of caspases-3 and caspases-7 (Chiang et al. 2003 ; Chou et al.…”

Section: Ssdmentioning

confidence: 99%

“… 2014a, 2014b ; Yao et al. 2014 ), suppresses the expression of COX-2, which has been shown to be involved in carcinogenesis (Lu et al. 2012b ; He et al.…”

Section: Ssdmentioning

confidence: 99%

“… 2003 ), anaplastic thyroid cancers cells ARO, 8305C, and SW1736 (Liu & Li 2014 ), prostate carcinoma cells DU145 (Yao et al. 2014 ), lung cancer cells A549 (Hsu et al. 2004a ), cervical carcinoma cells Hela (Wong et al.…”

Section: Ssdmentioning

confidence: 99%

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A systematic review of the active saikosaponins and extracts isolated from Radix Bupleuri and their applications

Yuan

Yang

et al. 2016

Pharmaceutical Biology

154

111

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Context: Radix Bupleuri has been used in traditional Chinese medicine for over 2000 years with functions of relieving exterior syndrome, clearing heat, regulating liver-qi, and lifting yang-qi. More natural active compounds, especially saikosaponins, have been isolated from Radix Bupleuri, which possess various valuable pharmacological activities.Objective: To summarize the current knowledge on pharmacological activities, mechanisms and applications of extracts and saikosaponins isolated from Radix Bupleuri, and obtain new insights for further research and development of Radix Bupleuri.Methods: PubMed, Web of Science, Science Direct, Research Gate, Academic Journals and Google Scholar were used as information sources through the inclusion of the search terms ‘Radix Bupleuri’, ‘Bupleurum’, ‘saikosaponins’, ‘Radix Bupleuri preparation’, and their combinations, mainly from the year 2008 to 2016 without language restriction. Clinical preparations containing Radix Bupleuri were collected from official website of China Food and Drug Administration (CFDA).Results and conclusion: 296 papers were searched and 128 papers were reviewed. A broad spectrum of in vitro and in vivo research has proved that Radix Bupleuri extracts, saikosaponin a, saikosaponin d, saikosaponin c, and saikosaponin b2, exhibit evident anti-inflammatory, antitumor, antiviral, anti-allergic, immunoregulation, and neuroregulation activities mainly through NF-κB, MAPK or other pathways. 15 clinical preparations approved by CFDA remarkably broaden the application of Radix Bupleuri. The main side effect of Radix Bupleuri is liver damage when the dosage is excess, which indicates that the maximum tolerated dose is critical for clinical use of Radix Bupleuri extract and purified compounds.

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“… 2014a, 2014b ; Yao et al. 2014 ), activates caspases apoptosis pathway, including the activation of caspases-3 and caspases-7 (Chiang et al. 2003 ; Chou et al.…”

Section: Ssdmentioning

confidence: 99%

“… 2014a, 2014b ; Yao et al. 2014 ), suppresses the expression of COX-2, which has been shown to be involved in carcinogenesis (Lu et al. 2012b ; He et al.…”

Section: Ssdmentioning

confidence: 99%

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A systematic review of the active saikosaponins and extracts isolated from Radix Bupleuri and their applications

Yuan

Yang

et al. 2016

Pharmaceutical Biology

154

111

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“…Surgical intervention can do some help to early stage RCC only, while patients with advanced tumor are still in urgent need for more promising approaches. Although studies have proved that SSd can inhibit cell growth and induce apoptosis in malignancies such as liver cancer, prostate cancer and thyroid carcinoma [8,23,24], its effect in RCC remains unclear. Our study is the first to explore the role and mechanism of SSd in RCC treatment which has been proved in this research to be able to inhibit RCC cell growth in vitro.…”

Section: Discussionmentioning

confidence: 99%

Saikosaponin-d suppresses cell growth in renal cell carcinoma through EGFR/p38 signaling pathway

Cai¹,

Zhang²,

Ou³

et al. 2017

neo

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The study aimed to explore the effect of Saikosaponin-d (SSd) and its underlying mechanism on cell growth inhibition as well as induction of apoptosis and cell cycle arrest in renal cell carcinoma (RCC). MTT assay and colony formation assay were employed in this study, with the results indicating that RCC cells proliferation was inhibited by SSd at different doses. Analysis by flow cytometry revealed that RCC cell proliferation inhibitory effect of SSd was achieved by inducing apoptosis and cell cycle arrest at G0/G1 phase via up-regulation of p53. As compared to the control group, SSd can significantly inhibit the growth of 769-P and 786-O cell lines and induce apoptosis and cell cycle arrest. The mechanism exploration demonstrated that inhibiting the activation of EGFR/p38 signaling pathways was the molecular basis of SSd's biological effects such as inducing apoptotic death, inhibiting cell growth as well as up-regulating p53 expression in human RCC cells. In conclusion, our data suggest that SSd may serve as a promising intervention for chemopreventive or chemotherapeutic treatment for patients with RCC.

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“…SSd has also been found to inhibit cell growth of human lung cancer A549 cells and human hepatoma HepG2 and Hep3B cells by induction of p53/p21mediated G1 phase cell cycle arrest and activation of Fas/FasL apoptotic signaling pathway (Hsu et al, 2004). Recent studies further demonstrated that downregulation of CDK2/cyclin D1 and P53/P21mediated cell cycle arrest at G0/G1 phase decrease the potential of mitochondrial membrane, and release of cytochrome c are critical for SSd-induced inhibition of human prostate cancer Du145 cells growth and apoptosis of human undifferentiated thyroid carcinoma cells (Yao et al, 2014;Liu and Li, 2014). Several studies also indicated that SSd mediates the apoptosis of human colon cancer HT29 cells via regulation of TRAIL, TRAIL-R, caspase-10 and caspase-8 signaling (Lu et al, 2013).…”

Section: Effects Of Sss On Cancersmentioning

confidence: 99%

A comprehensive review and perspectives on pharmacology and toxicology of saikosaponins

Huang

et al. 2018

Phytomedicine

138

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Background: Radix Bupleuri (RB) has been widely used in Chinese Traditional Medicine for over 2000 years and is currently marketed in China as Chai-Hu-Shu-Gan tablets and Xiao-Yao-Wan tablets. Saikosaponins (SSs, especially SSa, SSc and SSd), as the major bioactive compounds in RB, represent anti-inflammatory, anti-tumor, anti-oxidant, anti-viral and hepatoprotective effects. Purpose: To summarize recent findings regarding to the extraction, detection, biosynthesis, metabolism, pharmacological/toxicological effects of SSs. Methods: Online academic databases (including PubMed, Google Scholar, Web of Science and CNKI) were searched using search terms of "Saikosaponin", "Radix Bupleuri", "Bupleurum" and combinations to include published studies of SSs primarily from 2003 to 2018. Several critical previous studies beyond this period were also included. Results: 354 papers were found and 165 papers were reviewed. SSs have drawn great attention for their antiinflammation, anti-viral and anti-cancer effects and contradictory roles in the regulation of cell apoptosis, oxidative stress and liver fibrosis. Meanwhile, increased risks of overdose-induced acute or accumulation-related chronic hepatotoxicity of SSs and RB have also been reported. However, underlying mechanisms of SSs bioactivities, the metabolism of SSs and bioactivities of SSs metabolites are largely unknown. Conclusion: This comprehensive review of SSs provides novel insights and perspectives on the limitations of current studies and the importance of metabolism study and the dose-pharmacological/toxic relationship of SSs for the future discovery of SSs-based therapeutic strategies and clinical safe practice.

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Saikosaponin-d inhibits proliferation of DU145 human prostate cancer cells by inducing apoptosis and arresting the cell cycle at G0/G1 phase

Cited by 43 publications

References 40 publications

A systematic review of the active saikosaponins and extracts isolated from Radix Bupleuri and their applications

A systematic review of the active saikosaponins and extracts isolated from Radix Bupleuri and their applications

Saikosaponin-d suppresses cell growth in renal cell carcinoma through EGFR/p38 signaling pathway

A comprehensive review and perspectives on pharmacology and toxicology of saikosaponins

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