Species and sex identification of Formosa landlocked salmon using loop‐mediated isothermal amplification

Hsu, Te‐Hua; Adiputra, Yudha Trinoegraha; Ohta, Hiromi; Gwo, Jin‐Chywan

doi:10.1111/j.1755-0998.2011.03019.x

Cited by 13 publications

(11 citation statements)

References 21 publications

(44 reference statements)

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“…Hsu et al reported that excess DNA template (> 50 ng) should be avoided when carrying out an LAMP reaction. 10 In order to prevent a false-positive result due to contamination, some samples may need to be diluted before testing to reduce the incidence of false-positive.…”

Section: Discussionmentioning

confidence: 99%

Development of Reverse-Transcription Loop-Mediated Isothermal Amplification for Avian Leukosis Virus Subgroups a and J

Wang

2014

Taiwan Vet J

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Neoplasic diseases in chickens are frequently caused by avian leukosis virus (ALV), including subgroup A (ALV-A) and subgroup J (ALV-J) mostly. The objective of the present study was to develop a one-step, single-tube reverse transcription loop-mediated isothermal ampli¯cation (RT-LAMP) test for detecting ALV-A and ALV-J separately. Primers were designed from the conserved region of the pol and env genes of ALV-A and ALV-J for RT-LAMP tests. Other avian viruses were used to determine the speci¯city of these tests. The results showed that a ladder pattern on gel electrophoresis was observed for ALV-A and ALV-J speci¯cally and no crossreaction was observed with other avian viruses. Serial dilutions of ALV-A and ALV-J were used to evaluate the detection limits of these tests. The present RT-LAMP tests showed 10-100 times more sensitivity than the conventional reverse transcription polymerase chain reaction. Thus, these tests are rapid and accurate for the detection of ALV-A and ALV-J.

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Section: Discussionmentioning

confidence: 99%

Development of Reverse-Transcription Loop-Mediated Isothermal Amplification for Avian Leukosis Virus Subgroups a and J

Wang

2014

Taiwan Vet J

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“…) (among others) and applied in poultry or species with some commercial interest (Hsu et al . ; Chan et al . ; Kim et al .…”

Section: Introductionmentioning

confidence: 99%

Sex determination in the wild: a field application of loop‐mediated isothermal amplification successfully determines sex across three raptor species

Centeno‐Cuadros

Abbasi

Nathan

2016

Molecular Ecology Resources

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18PCR-based methods are the most common technique for sex determination of birds. 19Although these methods are fast, easy and accurate, they still require special facilities 20 that preclude their application outdoors. Consequently, there is a time lag between 21 sampling and obtaining results that impedes researchers to take decisions in situ and in 22 real time considering individuals' sex. We present an outdoor technique for sex 23 determination of birds based on the amplification of the duplicated sex-chromosome-24 specific gene Chromo-Helicase-DNA binding protein using a Loop-Mediated 25Isothermal Amplification (LAMP). We tested our method on Griffon Vulture (Gyps 26 fulvus), Egyptian Vulture (Neophron percnopterus) and Black Kite (Milvus migrans) 27 (family Accipitridae). We introduce the first fieldwork procedure for sex determination 28 of animals in the wild, successfully applied to raptor species of three different 29 subfamilies using the same specific LAMP primers. This molecular technique can be 30 deployed directly in sampling areas since it only needs a voltage inverter to adapt a 31 thermo-block to a car lighter and results can be obtained by the unaided eye based on 32 colour change within the reaction tubes. Primers and reagents are prepared in advance 33 to facilitate their storage at room temperature. We provide detailed guidelines how to 34 implement this procedure, which is simpler (no electrophoresis required), cheaper and 35 faster (results in ca. 90 minutes) than PCR-based laboratory methods. Our successful 36 cross-species application across three different raptor subfamilies posits our set of 37 markers as a promising tool for molecular sexing of other raptor families and our field 38 protocol extensible to all bird species.

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“…As morphological differences are very minimal in salmon. Hsu, et al (2011) designed the LAMP assay for the same. This is the first report where LAMP was successfully developed.…”

Section: Sex Determination In Endangered Speciesmentioning

confidence: 99%

Loop-Mediated Isothermal Amplification Assay (LAMP): A Rapid Tool for Diagnosis of Foodborne and Zoonotic Pathogens: A Review

Zende¹,

Kshirsagar²,

Vaidya³

et al. 2017

Int. J. Livest. Res.

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Species and sex identification of Formosa landlocked salmon using loop‐mediated isothermal amplification

Cited by 13 publications

References 21 publications

Development of Reverse-Transcription Loop-Mediated Isothermal Amplification for Avian Leukosis Virus Subgroups a and J

Development of Reverse-Transcription Loop-Mediated Isothermal Amplification for Avian Leukosis Virus Subgroups a and J

Sex determination in the wild: a field application of loop‐mediated isothermal amplification successfully determines sex across three raptor species

Loop-Mediated Isothermal Amplification Assay (LAMP): A Rapid Tool for Diagnosis of Foodborne and Zoonotic Pathogens: A Review

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