Abstract:Neoplasic diseases in chickens are frequently caused by avian leukosis virus (ALV), including subgroup A (ALV-A) and subgroup J (ALV-J) mostly. The objective of the present study was to develop a one-step, single-tube reverse transcription loop-mediated isothermal ampli¯cation (RT-LAMP) test for detecting ALV-A and ALV-J separately. Primers were designed from the conserved region of the pol and env genes of ALV-A and ALV-J for RT-LAMP tests. Other avian viruses were used to determine the speci¯city of these te… Show more
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