Speciation, Species Delineation, and Electrophoretic Isoenzyme Patterns of the Type Strains of Kluyveromyces van der Walt emend, van der Walt

Sidenberg, Deborah Gayle; Lachance, Marc‐André

doi:10.1099/00207713-33-4-822

Cited by 26 publications

(19 citation statements)

References 9 publications

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“…The members of this significant group are highly related and appear clearly separated from the other Kluyveromyces species. This is consistent with the ability of these strains to mate in the laboratory (Johannsen, 1980) and with the results of DNA base composition and relatedness (Fuson et al, 1987 ;Vaughan-Martini & Martini, 1987), isoenzyme analysis (Sidenberg & Lachance, 1983 and karyotyping (Sor & Fukuhara, 1989 ;Viljoen et al, 1989), although there is evidence of heterogeneity in these species at the intraspecific level (Cottrell et al, 1987 ;Belloch et al, 1997 ;Lehmann et al, 1992 ;Molnar et al, 1996). A second group includes the species K. africanus, Kluyveromyces delphensis, Kluyveromyces lodderae, K. polysporus and Kluyveromyces yarrowii, which appear intermixed with species belonging to the genera Saccharomyces, Torulaspora and Zygosaccharomyces in a polyphyletic group.…”

Section: Belloch and Otherssupporting

confidence: 88%

“…waltii species pair has been related to the K. marxianus group with respect to interfertility assays (Johannsen, 1980) and electrophoretic karyotyping (Belloch et al, 1998b). The electrophoretic isozyme patterns of Sidenberg & Lachance (1983) also indicated that K. thermotolerans shares with K. wickerhamii and K. dobzhanskii a more or less intermediate status between K. marxianus and K. lactis. Moreover, these workers also found a singular and unexpectedly high level of electrophoretic similarity between K. waltii and Kluyveromyces bulgaricus (now included in the taxon K. marxianus).…”

Section: Belloch and Othersmentioning

confidence: 95%

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Phylogeny of the genus Kluyveromyces inferred from the mitochondrial cytochrome-c oxidase II gene.

Belloch¹,

Querol²,

García³

et al. 2000

International Journal of Systematic and Evolutionary Microbiology

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A phylogenetic analysis of 17 species belonging to the genus Kluyveromyces and 12 reference and outgroup species was performed using mitochondrial cytochrome-c oxidase II gene sequences. The genus Kluyveromyces appears as a polyphyletic taxon formed by species included within the following four main groups. The Kluyveromyces phaffii group encompasses the species Kluyveromyces blattae, K. phaffii and Kluyveromyces yarrowii. The Kluyveromyces marxianus group is a monophyletic group consisting of the species Kluyveromyces aestuarii, Kluyveromyces dobzhanskii, Kluyveromyces lactis, K. marxianus and Kluyveromyces wickerhamii. The monophyletic Kluyveromyces thermotolerans group is formed by K. thermotolerans, Kluyveromyces waltii and Saccharomyces kluyveri (which appears in the mitochondrial tree as the sister clade of the K. marxianus group). Finally, the Saccharomyces cerevisiae group contains the remaining Kluyveromyces species, as well as the reference Saccharomyces species (sensu lato and sensu stricto) and Candida glabrata (the phylogenetic relationships within this group are unclear according to the bootstrap test). The phylogenetic relationships obtained for this mitochondrial gene are, for the most part, congruent with previous trees based on nuclear rRNA sequences, except for the position of K. yarrowii and the close relationship between the K. marxianus and K. thermotolerans groups. These differences, as well as the existence of these groups, are discussed in the context of previous studies based on phenotypic, genetic and molecular data. Although additional studies are required to decipher the phylogenetic relationships between the genus Kluyveromyces and the closely related genera Saccharomyces, Torulaspora and Zygosaccharomyces, future changes to their taxonomic status should take account of the existence of these four groups of Kluyveromyces species.

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Section: Belloch and Otherssupporting

confidence: 88%

Section: Belloch and Othersmentioning

confidence: 95%

Phylogeny of the genus Kluyveromyces inferred from the mitochondrial cytochrome-c oxidase II gene.

Belloch¹,

Querol²,

García³

et al. 2000

International Journal of Systematic and Evolutionary Microbiology

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“…Isoenzyme analysis has shown that K . aestuarii has many unique electromorphs, not found in any other Kluyveromyces species (32). Further, the exo-P-glucanases of K .…”

Section: Resultsmentioning

confidence: 92%

“…Sidenberg and Lachance (32,33) have published two studies of isoenzyme variation in Kluyveromyces. The first study included the type strains of all Kluyveromyces species (32). The second study used numerous isolates of K .…”

Section: Resultsmentioning

confidence: 99%

Deoxyribonucleic Acid Base Sequence Relatedness among Members of the Yeast Genus Kluyveromyces

Fuson

Presley

Phaff

1987

International Journal of Systematic Bacteriology

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Deoxyribonucleic acid (DNA) base composition and DNA base sequence relatedness comparisons were used for species delineation in the genus Kluyveromyces. Base composition values separated the members of the genus into three groups. The groups were further subdivided by comparing base sequences by using DNAIDNA renaturation experiments. Two DNA homology groups were identified. The first group included Kluyveromyces marxianus, Kluyveromyces fragilis, Kluyveromyces bulgaricus, Kluyveromyces cicerisporus, Kluyveromyces wikenii, and three anamorphs (Candida kefyr, Candida pseudotropicalis, and Torula cremoris); the members of this group exhibited 290 % DNA base sequence complementarity. The second group consisted of Kluyveromyces lactis, Kluyveromyces vanudenii, Kluyveromyces drosophilarum, and Kluyveromyces phaseolosporus; various pairs of these yeasts shared 64 to 98% of their DNA sequences. The two groups were only distantly related to each other (115% DNA base sequence complementarity). The other Kluyveromyces species appear to be unique, not being closely related to either of the two homology groups or to one another. Relationships deduced from comparisons of DNAs agreed well with those deduced by other workers from immunological comparisons of exo-P-glucanases and from isoenzyme analysis but were only in partial agreement with a taxonomic arrangement made on the basis of mating studies. We propose recognition of the following species:

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“…Several subsequent approaches to the taxonomy of the genus Kluyveromyces were attempted, such as those based on vitamin requirements (9), electrophoretic isoenzyme patterns (45,46), structure of exo-P-glucanases (23), chemical and immunochemical studies of cell wall components (7,13,31,40,51), numerical taxonomy (6, 3 3 , ultrastructure of the ascospore wall (2), type of coenzyme Q (62), interfertility studies (14, 15), and nuclear deoxyribonucleic acid (DNA) base composition (28,33,36).…”

mentioning

confidence: 99%

Taxonomic Revision of the Yeast Genus Kluyveromyces by Nuclear Deoxyribonucleic Acid Reassociation

Martini

1987

International Journal of Systematic Bacteriology

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Deoxyribonucleic acid relatedness among the type strains of all taxa and known anamorphs assigned to the yeast genus Kluyveromyces was assessed by the optical reassociation technique. Three groups of species related at the 95% level or higher were found: (i) K. lactis including K . drosophilarum, K. phaseolosporus, K . vanudeni, and the anamorph Candida sphaerica; (ii) K . marxianus with K. bulgaricus, K. cicerisporus, K . fragilis, K . wikenii, and the anamorphs Candida kefyr, Candida macedoniensis, and Candida pseudotropicalis; (iii) K. thermotolerans with K. veronae and the anamorph Candida dattila. The remaining species, including the recently described K . blattae and K . waltii, are not related to each other or to the members of the above three groups. The nomen nudum K. cellobiovorus is not conspecific with any of the species of the genus. The species assignment obtained by nuclear deoxyribonucleic acid reassociation does not entirely conform with the previously proposed reorganization of the genus Kluyveromyces (J.-P. van der Walt and E. Johannsen, p. 224-251, in N. J. W. Kreger-van Rij, ed., The Yeasts. A Taxonomic Study, 1984).The genus Kluyveromyces was established in 1956 by van der Walt (52) to classify the newly isolated, budding, fermentative yeast species K. polysporus, which produces large, multispored asci containing as many as 70 or more reniform to long oval spores. In the same year, an additional species, K. africanus, forming up to 16 spores per ascus, was included in the genus by van der Walt (53). The salient property of the asci of these two species was their early evanescence at maturity, to release the spores.In 1965, the diagnosis of the genus was emended by van der Walt (54) on the assumption that the species with multispored asci represented only a separate line of development in relation to other species which normally form only four spores. The above approach was based on a series of controversial taxonomic treatments of those yeast species that form kidney-shaped spores (5, 18, 19, 34,60). Van der Walt included in Kluyveromyces all the species formerly classified in the genera Fahospora, Zygofahospora Kudriawzev (19), Dekkeromyces nomen nudum Wic kerham and Burton (59), and Guilliermondella (5). Later, Santamaria and Sanchez (41) proposed to assign the nomen nudum Dekkeromyces to those species of the genus that are unable to form multispored asci (K. dobzhanskii, K. drosophilarum, K . fragilis, K . lactis, K . phaseolosporus, and K . wickerhamii). Van der Walt (55) applied the overall criteria that had suggested the earlier emendation and assigned to the genus 19 species including several recently described new taxa.Several subsequent approaches to the taxonomy of the genus Kluyveromyces were attempted, such as those based on vitamin requirements (9), electrophoretic isoenzyme patterns (45, 46), structure of exo-P-glucanases (23), chemical and immunochemical studies of cell wall components (7,13, 31,40,51), numerical taxonomy (6, 3 3 , ultrastructure of the ascospore wall (2), t...

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Speciation, Species Delineation, and Electrophoretic Isoenzyme Patterns of the Type Strains of Kluyveromyces van der Walt emend, van der Walt

Cited by 26 publications

References 9 publications

Phylogeny of the genus Kluyveromyces inferred from the mitochondrial cytochrome-c oxidase II gene.

Phylogeny of the genus Kluyveromyces inferred from the mitochondrial cytochrome-c oxidase II gene.

Deoxyribonucleic Acid Base Sequence Relatedness among Members of the Yeast Genus Kluyveromyces

Taxonomic Revision of the Yeast Genus Kluyveromyces by Nuclear Deoxyribonucleic Acid Reassociation

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