Speciation of Organic and Inorganic Selenium in Selenium-enriched Eggs by Hydride Generation Atomic Fluorescence Spectrometry

Sun, Hanwen; Feng, Bo

doi:10.1007/s12161-010-9145-5

Cited by 19 publications

(8 citation statements)

References 18 publications

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“…The concentrations of SeCys 2 and SeMet were 81.80 ± 0.53 µg kg −1 and 6.96 ± 0.20 µg kg −1 (as Se), respectively. According to reports, the intake of Se converted by hen organism was bonded to protein and present mainly in organic forms in eggs [13,14]. The results of Se species extracted from egg by enzymatic hydrolysis were consistent with this conclusion.…”

Section: Evaluation Of Different Sample Extraction Proceduressupporting

confidence: 78%

“…The detection limits were 60, 3, and 10 µg kg −1 (dry weight) for SeCys, Se(IV), and SeMet, respectively, and the precision was 5-10% [13]. Sun and Feng developed a method using HG-AFS by depositing albumen with trichloroacetic acid to determination organic and inorganic Se in fresh Se-enriched eggs [14]. Up to now, there were few reports about the analysis method of Se species in fresh liquid whole egg.…”

Section: Introductionmentioning

confidence: 99%

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Determination of Selenomethionine, Selenocystine, and Methylselenocysteine in Egg Sample by High Performance Liquid Chromatography—Inductively Coupled Plasma Mass Spectrometry

et al. 2022

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The deficiency of selenium in dietary is recognized as a global problem. Eggs, as one of the most widely consumed food products, were readily enriched with selenium and became an important intake source of selenium for humans. In order to better understand the speciation and bioaccessibility of selenium in eggs, a simple and reliable approach that could be easily used in a routine laboratory was attempted to develop for analyzing selenium species. Three of organic selenium species (selenocystine, methylselenocysteine, and selenomethionine) in liquid whole egg were completely released by enzymatic hydrolysis and detected by high performance liquid chromatography in combination with inductively coupled plasma mass spectrometry (HPLC-ICP-MS). All the parameters in enzymatic hydrolysis and separation procedures were optimized. The effect of matrix in analysis was critically evaluated by standard addition calibrations and external calibrations. Under the optimal conditions, the spike recoveries of selenium species at 0.1–0.4 μg g−1 spike levels all exceeded 80%. This method was successfully applied to the determination of selenium species in fresh egg and cooked eggs.

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Section: Evaluation Of Different Sample Extraction Proceduressupporting

confidence: 78%

Section: Introductionmentioning

confidence: 99%

Determination of Selenomethionine, Selenocystine, and Methylselenocysteine in Egg Sample by High Performance Liquid Chromatography—Inductively Coupled Plasma Mass Spectrometry

et al. 2022

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“…(2005a) so ugotavljali tudi vsebnost selena v ribah, kjer ga je 15,3-68,6 µg/100 g, v piščančjem mesu 9,7-15,4 µg/100 g) in v puranjem mesu 9,9-11,6 µg/100 g. Jajca v povprečju vsebujejo 26 µg selena v 100 g vzorca. Z dodatkom selena h krmni mešanici za kokoši je možno vsebnost selenometionina in selenocisteina v jajcih močno povečati (Sun in Feng, 2011).…”

Section: Hrana žIvalskega Izvora In Prehranski Dodatkiunclassified

Plant Resources Based Selenium Supplementation in Daily Nutrition

Škrabanja¹

2017

AAS

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IZVLEČEKSelen je eden izmed esencialnih elementov, potrebnih za človekovo zdravje. Razmere v okolju in kmetijska praksa imajo velik vpliv na vsebnost selena v rastlinah. Gojenje rastlin, obogatenih s selenom, je učinkovit način dodajanja selena v vsakdanjo prehrano tistih, ki tega esencialnega elementa ne prejmejo v zadostnih količinah. Biorazpoložljivost selena je v korelaciji z izvorom in s kemijsko obliko, v kateri je ta prisoten v živilu. S prehranskega vidika so s selenom obogatena živila potencialni dodatni vir selena, ki se vnaša v organizem preko prehranske verige in se s kompleksnimi pretvorbami na molekulski ravni pretvarja iz anorganskih v bolj razpoložljive organske oblike. V članku so zbrani rezultati sistematičnega raziskovanja vsebnosti selena v posameznih rastlinskih vrstah in različnih načinov dodajanja selena za dosego večje koncentracije letega v pridelku. V Sloveniji so tla s selenom revna in je možnost pomanjkanja selena v prehrani nekaterih skupin prebivalstva velika. Zato je v članku večji poudarek namenjen pregledu objavljenih del domačih strokovnjakov, ki so vsebnost selena spremljali ob simulaciji sprememb okoljskih dejavnikov, kar je dodatno vplivalo na fiziološke lastnosti in pridelek rastlin. Upoštevana je pozitivna vloga selena v presnovi človeka ter njegov vpliv na zdravje. Omenjeni so tudi negativni vplivi zaradi pomanjkanja ali presežka selena v hrani, ki opozarjajo na to, da je treba priporočila v zvezi z vnosom selena v naš organizem temeljito spremljati in premišljeno prilagajati referenčne vrednosti.Ključne besede: selen; rastlinski viri; prehranski vnos; dodatek selena ABSTRACT PLANT RESOURCES BASED SELENIUM SUPPLEMENTATION IN DAILY NUTRITIONSelenium is one of the essential elements that has a direct effect on human health and disease. Environmental conditions and agricultural practice have a profound influence on the selenium content in plants. Cultivation of plants enriched with the selenium has an effective potential for selenium supplementation in diets for population which is exposed to selenium deficiency. Bioavailability of selenium compounds from food is in strong correlation with the source and its chemical form. The selenium of different sources and forms can become a part of human consumption when entering the food chain, wherein the inorganic forms of selenium are metabolized and converted to more available organic forms. Numerous results of systematic research of the selenium content in individual plant species as well as various techniques for producing selenium enriched foodstuffs is reviewed. The soil in Slovenia is selenium-poor and may concern a part of population which is potentially sensitive to selenium status. The merits of selenium effect, either alone or in combination with different environmental changes on plant production published by Slovenian authors are thus closely considered. Controversies continue to prevail regarding adequate amounts for selenium for health and disease prevention.Thus, general and individualized recommendations for selenium ...

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“…Heras et al 22 reviewed the diverse analytical techniques employed to elucidate the structures of Se-containing proteins and presented the best characterized proteins, highlighting the many challenges involved. The relative proportion of organic Se and iSe in enriched eggs was determined using a new HG-AFS method developed by Sun and Feng 175 giving an LOD of 0.07 mg L À1 . The amount of organic Se was 3 to 7 times that of iSe.…”

Section: Zincmentioning

confidence: 99%

Atomic spectrometry update. Clinical and biological materials, foods and beverages

Taylor¹,

Day²,

Marshall³

et al. 2012

J. Anal. At. Spectrom.

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Amid the traditional development and applications work reported during the period covered by this review, the most obvious identifiable trend is that of downsizing. This is seen with techniques requiring smaller sample sizes or more effective analyte pre-concentration, and with new instrumentation. Procedures for extraction included the use of nanoparticles in various ways and the single-drop methodology with one example exploiting 1-dodecanol, a material that shifts between liquid and

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Speciation of Organic and Inorganic Selenium in Selenium-enriched Eggs by Hydride Generation Atomic Fluorescence Spectrometry

Cited by 19 publications

References 18 publications

Determination of Selenomethionine, Selenocystine, and Methylselenocysteine in Egg Sample by High Performance Liquid Chromatography—Inductively Coupled Plasma Mass Spectrometry

Determination of Selenomethionine, Selenocystine, and Methylselenocysteine in Egg Sample by High Performance Liquid Chromatography—Inductively Coupled Plasma Mass Spectrometry

Plant Resources Based Selenium Supplementation in Daily Nutrition

Atomic spectrometry update. Clinical and biological materials, foods and beverages

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