Speciation of mercury in salmon egg cell cytoplasm in relation with metallomics research

Metallomics Research Related to Arsenic

“…The CHAPS-coated ODS column was prepared by the dynamic coating method. As reported previously [39,40], the CHAPS-coated ODS column has unique characteristics for simultaneous separation of ions or small molecules and large molecules (e.g., proteins) and the useful information about the elements binding or non binding with proteins is easily obtained. The chromatograms for salmon egg cytoplasm obtained by HPLC/ICP-MS using the CHAPS-coated ODS column are shown in Figure 4.9.…”

“…Copyright 2008 IUPAC together with those with the UV-absorption detection at 254 nm, where isotopes detected and elemental concentrations determined by ICP-MS are also indicated in each chromatogram. It should be noted here that the retention range of 3.0-3.5 min shown as the gray zones in Figure 4.9 indicates the elution zone of large molecules with molecular weights (MW) greater than about 10 kDa, while the retention range after 3.5 min indicates the elution zone of small ions and molecules [40]. However, the molecular weights of the eluted molecules can not be estimated from the chromatograms obtained by the CHAPS-coated ODS column, since the CHAPS-coated ODS column has no size exclusion property.…”

Metallomics of Mercury: Role of Thiol‐ and Selenol‐Containing Biomolecules

“…When coupled with atomic (e.g., by ICP) or molecular (e.g., by electrospray ionization, ESI) MS, HPLC-MS provides a powerful means for the separation and quantification of Hg and MeHg species. For example, the presence of Hg(SeCys) 2 and/or Hg(SCys) 2 in salmon egg cell cytoplasm (Hasegawa et al, 2005) and the in vitro and in vivo formation of SelP-bound (HgSe) n (Suzuki et al, 1998) were all reported on the basis of HPLC-ICP-MS evidence. Hintelmann and Simmons (2003) were the first to use ESI-MS to study MeHg speciation in aqueous solutions.…”

Metallomics study on all-elements analysis of salmon egg cells and fractionation analysis of metals in cell cytoplasm

“…, where the ICP-MS was also used as the element-selective detector. The CHAPS-coated ODS column was prepared by the dynamic coating method, as described in previous papers [4,[19][20][21], and has unique characteristics for simultaneous separation of large and small ions/molecules, as mentioned later. The size exclusion chromatography (SEC) column used for protein separation was of Superose 12HR 10/300 GL (Amersham Bioscience, Piscataway, NJ, USA) with a molecular permeation range of 1-300 kDa, whose calibration for molecular weights (MWs) was performed using standard proteins, as usual [22,23].…”

“…4 ppb and ca. 8 ppb of Hg exist as cysteine-and protein-binding species, respectively [21]. The existence of organoarsenic species, amino acid, and protein complexes suggests that salmon egg cells have access to detoxification mechanisms for these toxic elements.…”