Speciation of eight arsenic compounds in human urine by high-performance liquid chromatography with inductively coupled plasma mass spectrometric detection using antimonate for internal chromatographic standardization

Larsen, Erik Huusfeldt; Pritzl, Gunnar; Hansen, Steen Honoré

doi:10.1039/ja9930800557

Cited by 118 publications

(64 citation statements)

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“…The current reported method for determination of six arsenic species in urine using an ammonium carbonate buffer resulted in acceptable performance and significant improvement over the existing methods reported in the literature (6)(7)(8)(9)(10)(11)(12)(13)(14). The method uses cationic and anionic columns in series for complete baseline separation of six arsenic species and the internal standard, in a single chromatographic run of less than 30 min.…”

Section: Discussionmentioning

confidence: 93%

“…There have been many studies involving speciation by the coupling of ion-exchange chromatography (IC) to ICP-MS (IC-ICP-MS) (6,7); however, studies reported in the literature have employed two separate columns (anionic and cationic). Using a single column to separate the six aforementioned arsenic species is difficult because AsC exists in cationic form at most pH levels, whereas the other five species can be made anionic by adjusting the pH.…”

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confidence: 99%

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Development and application of a robust speciation method for determination of six arsenic compounds present in human urine.

Milstein

Essader

Pellizzari

et al. 2003

Environ Health Perspect

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Six arsenic species [arsenate, arsenite, arsenocholine, arsenobetaine, monomethyl arsonic acid, and dimethyl arsinic acid] present in human urine were determined using ion-exchange chromatography combined with inductively coupled plasma mass spectrometry (IC-ICP-MS). Baseline separation was achieved for all six species as well as for the internal standard (potassium hexahydroxy antimonate V) in a single chromatographic run of less than 30 min, using an ammonium carbonate buffer gradient (between 10 and 50 mM) at ambient temperature, in conjunction with cation- and anion-exchange columns in series. The performance of the method was evaluated with respect to linearity, precision, accuracy, and detection limits. This method was applied to determine the concentration of these six arsenic species in human urine samples (n = 251) collected from a population-based exposure assessment survey. Method precision was demonstrated by the analysis of duplicate samples that were prepared over a 2-year analysis period. Total arsenic was also determined for the urine samples using flow injection analysis coupled to ICP-MS. The summed concentration of the arsenic species was compared with the measured arsenic total to demonstrate mass balance.

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Section: Discussionmentioning

confidence: 93%

mentioning

confidence: 99%

Development and application of a robust speciation method for determination of six arsenic compounds present in human urine.

Milstein

Essader

Pellizzari

et al. 2003

Environ Health Perspect

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“…Upon arrival at the University of Arizona, urine samples were transferred to 15-mL tubes and frozen at −20°C until analysis (within 1 mo of collection). Samples were analyzed for individual species of urinary arsenic using a high-performance liquid chromatography (HPLC) ICP-MS system (37)(38)(39). This system uses an HPLC pump (Dionex GP50 pump) and an HPLC column (Hamilton PRP100X) connected to a collision cell ICP mass spectrometer.…”

Section: Methodsmentioning

confidence: 99%

Rice consumption contributes to arsenic exposure in US women

Gilbert‐Diamond

Cottingham

Gruber

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

320

276

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Emerging data indicate that rice consumption may lead to potentially harmful arsenic exposure. However, few human data are available, and virtually none exist for vulnerable periods such as pregnancy. Here we document a positive association between rice consumption and urinary arsenic excretion, a biomarker of recent arsenic exposure, in 229 pregnant women. At a 6-mo prenatal visit, we collected a urine sample and 3-d dietary record for water, fish/ seafood, and rice. We also tested women's home tap water for arsenic, which we combined with tap water consumption to estimate arsenic exposure through water. Women who reported rice intake (n = 73) consumed a median of 28.3 g/d, which is ∼0.5 cup of cooked rice each day. In general linear models adjusted for age and urinary dilution, both rice consumption (g, dry mass/d) and arsenic exposure through water (μg/d) were significantly associated with natural log-transformed total urinary arsenic (β rice = 0.009, β water = 0.028, both P < 0.0001), as well as inorganic arsenic, monomethylarsonic acid, and dimethylarsinic acid (each P < 0.005). Based on total arsenic, consumption of 0.56 cup/d of cooked rice was comparable to drinking 1 L/d of 10 μg As/L water, the current US maximum contaminant limit. US rice consumption varies, averaging ∼0.5 cup/d, with Asian Americans consuming an average of >2 cups/d. Rice arsenic content and speciation also vary, with some strains predominated by dimethylarsinic acid, particularly those grown in the United States. Our findings along with others indicate that rice consumption should be considered when designing arsenic reduction strategies in the United States.

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“…Similar results were found in heart tissues of chickens (46). AsB is believed to have a very low toxicity and has been found in human serum (9); the presence of arsenobetaine was unexpected in this work; this organarsenical species enters human beings principally from marine foods and from agricultural products coming from In vivo methylation has long been proposed as an arsenic detoxification pathway for inorganic arsenic (22,47,48), but it has recently been shown that As(III)-MMA and As(III)-DMA, two intermediates in arsenate methylation, are more toxic to cells than As 3+ and A 5+ ions (22,47,49,50). Arsenic is primarily metabolized in the liver and cardiovascular tissue probably does not efficiently metabolize arsenic.…”

Section: +mentioning

confidence: 99%

Environmental Contamination of Arsenic and its Toxicological Impact in Cardiovascular Tissues of Patients with Coronary Heart Disease

2017

IJSR

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Background: The concentration levels of As in the Chilean II Region of Antofagasta produces non cancer health outcomes such as cardiovascular diseases and in last term heart attack. On this study, the determination of total As content and main inorganic and organoarsenic species found in three heart tissues (auricle, mammary artery and fat) and the saphenous vein of people living in the Chilean II Region, suffering coronary thrombosis has been carried out. Comparison with similar tissues of patients from other noncontaminated areas has been undertaken. Methods: Total As determination has been performed by HG-AAS and ICP-MS. For As species have been used the tandem HPLC-ICP-MS using the Hamilton PRP X100 anion column and methanol:water (1:1) extraction. Results: The auricle and in less extend the saphenous vein support the higher As concentration (mean values of 7.7 and 2.5 µg g -1 , respectively), being As(III) the predominant species. Methylation towards MMA and DMA is not a favoured mechanism. Conclusions: The presence of high total As and high As(III) species content in the auricle and saphenous vein of more contaminated people, the damage found in the saphenous vein tissue and the global characteristics of the people under study in which the As stigmas are present in all of them, suggests that As could be involved in the cardiovascular diseases.

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Speciation of eight arsenic compounds in human urine by high-performance liquid chromatography with inductively coupled plasma mass spectrometric detection using antimonate for internal chromatographic standardization

Cited by 118 publications

References 10 publications

Development and application of a robust speciation method for determination of six arsenic compounds present in human urine.

Development and application of a robust speciation method for determination of six arsenic compounds present in human urine.

Rice consumption contributes to arsenic exposure in US women

Environmental Contamination of Arsenic and its Toxicological Impact in Cardiovascular Tissues of Patients with Coronary Heart Disease

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