Speciation of bioaccessible (heme, ferrous and ferric) iron from school menus

Cámara, F.; Amaro, Manuel; Barberá, Reyes; Lagarda, Marı́a Jesús

doi:10.1007/s00217-005-0008-z

Cited by 15 publications

(10 citation statements)

References 26 publications

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“…In vitro systems usually consist of the simulated gastrointestinal digestion, followed by measurement of the dialyzable mineral fraction across a semi permeable membrane. [9][10][11] On the other hand, the in vivo methods involve the use of animals or human beings. In this case, the interaction of Se with other compounds presented in the organism influences its bioavailability.…”

Section: Introductionmentioning

confidence: 99%

“…In this case, the interaction of Se with other compounds presented in the organism influences its bioavailability. [9][10][11] Elemental speciation at the molecular level has been successfully performed by conventional proteomics approaches with the separation by size exclusion chromatography coupled to ultraviolet and inductively coupled plasma mass spectrometry (SEC-UV-ICP-MS), and identification by ion chromatographic-associated electrospray mass spectrometry (ESI-MS/MS) and matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). 12,13 Atomic absorption spectrometry (AAS), inductively coupled optical emission spectrometry (ICP OES) and inductively coupled plasma mass spectrometry (ICP-MS) have been applied for the elemental determination.…”

Section: Introductionmentioning

confidence: 99%

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In vivo bioavailability of selenium in enriched Pleurotus ostreatus mushrooms

et al. 2010

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The in vivo bioavailability of Se was investigated in enriched Pleurotus ostreatus mushrooms. A bioavailability study was performed using 64 Wistar male rats separated in 8 groups and fed with different diets: without Se, with mushrooms without Se, with enriched mushrooms containing 0.15, 0.30 or 0.45 mg kg(-1) Se and a normal diet containing 0.15 mg kg(-1) of Se using sodium selenate. The experiment was performed in two periods: depletion (14 days) and repletion (21 days), according to the Association of Official Analytical Chemists. After five weeks, the rats were sacrificed under carbon dioxide, and blood was drawn by heart puncture. Blood plasma was separated by centrifugation. The total Se concentration in the plasma of rats fed with enriched mushrooms was higher than in rats fed with a normal diet containing sodium selenate. The plasma protein profiles were obtained using size exclusion chromatography (SEC) and UV detectors. Aliquots of effluents (0.5 mL per minute) were collected throughout in the end of the chromatographic column. However, Se was determined by graphite furnace atomic absorption spectrometry (GF AAS) only in the aliquots where proteins were detected by SEC-UV. The plasma protein profile of rats fed with different diets was similar. The highest Se concentration was observed in a peptide presenting 8 kDa. Furthermore, the higher Se concentration in this peptide was obtained for rats fed with a diet using enriched mushrooms (7 μg L(-1) Se) compared to other diets (2-5 μg L(-1) Se). These results showed that Se-enriched mushrooms can be considered as an alternative Se food source for humans, due to their high bioavailability.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In vivo bioavailability of selenium in enriched Pleurotus ostreatus mushrooms

et al. 2010

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“…Use of this in vitro analytical strategy has been widely reported in the literature to estimate the bioaccessibility of nutrients in foods due to its cost effectiveness, speed, and safety, as well as the less stringent ethical restrictions associated with such methods as compared to those imposed on in vivo methods. 42,43 In addition, the procedure can be complemented via the use of a semipermeable membrane (model (ii)) in order to simulate the transport of nutrient into the human body. However, this procedure requires addition of a dialysis step to evaluate the concentration of elements that diffuse through the membrane.…”

Section: Thais T Mingroni a Juliana Hamada A Alexsandra D S Xavimentioning

confidence: 99%

In vitro Evaluation of Ca, Cu, and Mg Bioaccessibility in Fresh and Dried Fruits

Mingroni¹,

Hamada²,

Xavier³

et al. 2018

J. Braz. Chem. Soc.

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In this work, the bioaccessibility of Ca, Mg, and Cu in commercial dried fruits was evaluated in vitro, and compared with that in the corresponding fresh fruits. In fresh fruits, the bioaccessibility of Ca was found to be between 72.3 and 92.2%, while Mg bioaccessibility was observed to be in the range 59.5-66.3%. In dried fruits, the bioaccessibility of Mg was approximately 45%, while that of Ca was in the range of 12.2-52%. The average bioaccessibility of Ca in banana (dried fruit) was lower (12.2%) than papaya (22.9%) and apple (52%). In addition, for all samples, Cu content was below the limit of detection (LOD) (1.12 μg g-1), suggesting that Cu is present in the researched fruits in a chemical form that is poorly absorbed by the human body. Considering these results, it is possible to conclude that the bioaccessibility of Ca and Mg was significantly lower in dried fruits than in fresh fruits, whereas Cu bioaccessibility was below the LOD of the method. These results demonstrate that the dehydration process negatively affected the bioaccessibility of all elements evaluated in this study, reducing the amount of nutrients that can be absorbed by the human body.

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“…Nutrition studies reported that both iron species are bioavailable but Fe 2þ is more bioavailable than Fe 3þ because it is more soluble in the intestinal lumen [19,20]. The bioavailability of particulate iron depends, in general, on the size-fraction and sources of the aerosols [21].…”

Section: Introductionmentioning

confidence: 99%