Speciation of Aluminium in Environmental Water Samples Employing Microcolumn Chelating Ion-Exchange Chromatography - ETAAS

Complexation of labile aluminium species by chelating resins Iontosorb – a new method for Al environmental risk assessment

“…In the case of sulphate addition, more than 90% exchange was observed after 5 min complexation (Table 2). Similarly, Kožuh et al [34,35] obtained almost 100% recoveries of Al-sulphate employing microcolumn chelating ion-exchange chromatography with Chelex resin. It is caused by the presence of the positively charged monomeric aqua aluminium, hydroxoaluminium and aluminium sulphato ([AlSO 4 ] + ) complexes [3].…”

“…Generally, the Al fractions defined operationally according to the method of determination are termed labile or reactive aluminium. The reactive Al can be separated from less reactive species using ion-exchange on chelating resins Chelex [21,[32][33][34][35][36][37][38]. This separation manner can be accomplished either by short-term batch reaction with a resin or by using column techniques.…”

“…In most cases, these groups are termed as the operationally defined fractions (the species are defined by the analytical technique used for their separation). The mainly used techniques in the speciation analysis and fractionation of aluminium are the ion chromatography [30,31], size exclusion chromatography [31] and chelating ion exchange [21,[32][33][34][35][36][37][38][39]. Generally, the Al fractions defined operationally according to the method of determination are termed labile or reactive aluminium.…”

Speciation of trace amounts of aluminium in environmental samples by cation-exchange FPLC - ETAAS

“…Al(OH) 3 is eluted as a broad peak from 5.5 to 9.5 min and does not interfere with the separation of positively charged monomeric aluminium species. Spectrophotometry [6][7][8][9][10][11][12][13], chelating ion-exchange chromatography [14][15][16][17], ion-chromatography [18][19][20][21] and high performance liquid chromatography [22][23][24][25] are able to determine only the sum of labile monomeric aluminium species. The advantage of the present FPLC -ETAAS procedure lies in its ability to distinguish between Al 3+ , Al(OH) 2+ and Al(OH) + 2 monomeric aluminium species at low concentration levels, which is of great importance in toxicological investigations.…”

“…Labile positively charged monomeric forms of aluminium (Al 3+ , Al(OH) 2+ and Al(OH) 2 + ) have been recognised as the most toxic aluminium species [1][2][3][4][5]. Therefore, investigations were directed to the development of analytical methods which enable determination not only of the sum of positively charged monomeric aluminium species [6][7][8][9][10][11][12][13][14][15][16][17] but also allow distinction between particular aluminium forms [18][19][20][21][22][23][24][25][26][27]. In recent years ion chromatography in combination with post-column derivatization using UV-VIS [18,19] or fluorescence detection [20,21] were frequently employed for speciation of cationic aluminium complexes.…”