Specialized infection strategies of falcate and oval conidia of Colletotrichum graminicola

Nordzieke, Daniela Elisabeth; Sanken, Alina; Antelo, Luís T.; Raschke, Anja; Deising, H. B.; Pöggeler, Stefanie

doi:10.1016/j.fgb.2019.103276

Cited by 16 publications

(53 citation statements)

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“…Fungal Growth Conditions C. graminicola generates two distinct asexual spores, falcate and oval conidia, differing in morphology, germination behavior, and maize infection strategies (Panaccione et al, 1989;Nordzieke et al, 2019b). Since C. graminicola oval conidia are morphologically similar to microconidia of F. oxysporum used in former studies (Turrà et al, 2015;Nordzieke et al, 2019a;Vitale et al, 2019), we adapted fungal-specific conditions for oval conidia and optimized incubation time, water agar composition and spore concentration alike as the spore application procedure.…”

Section: Choice Of Chemoattractant Signalmentioning

confidence: 99%

“…After a total of 7 days, mycelia and liquid phase are separated by miracloth filtration. After two rounds of centrifugation (10 min, 7,000 rpm) and washing of the pellet with distilled water, oval conidia are quantified using a Neubauer counting chamber (Nordzieke et al, 2019b). Finally, spore concentration is adapted to c = 5 * 10 5 /ml in distilled water.…”

Section: Preparation Of the C Graminicola Inoculummentioning

confidence: 99%

“…Due the ability to infect several plant parts and its high epidemic spreading potential, this pathogen is estimated to cause crop losses up to 1 billion dollars/year (United States) corresponding to 40% per field (Perfect et al, 1999;Frey et al, 2011). C. graminicola generates two types of infectious asexual spores, falcate and oval shaped conidia (Nishihara, 1975;Panaccione et al, 1989), having distinct roles and following particular strategies during the infection cycle (Nordzieke et al, 2019b). As we recently reported, oval conidia develop a germling network by the formation of conidial anastomosis tubes (CATs) on maize leaves, which might contribute to their pathogenic potential (Nordzieke et al, 2019b).…”

Section: Introductionmentioning

confidence: 99%

“…C. graminicola generates two types of infectious asexual spores, falcate and oval shaped conidia (Nishihara, 1975;Panaccione et al, 1989), having distinct roles and following particular strategies during the infection cycle (Nordzieke et al, 2019b). As we recently reported, oval conidia develop a germling network by the formation of conidial anastomosis tubes (CATs) on maize leaves, which might contribute to their pathogenic potential (Nordzieke et al, 2019b). In contrast, falcate conidia germinate on short germ tubes on maize leaves followed by rapid development of appressoria for plant penetration (Mims and Vaillancourt, 2002).…”

Section: Introductionmentioning

confidence: 99%

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A 3D Printed Device for Easy and Reliable Quantification of Fungal Chemotropic Growth

2020

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Chemical gradients are surrounding living organisms in all habitats of life. Microorganisms, plants and animals have developed specific mechanisms to sense such gradients. Upon perception, chemical gradients can be categorized either as favorable, like nutrients or hormones, or as disadvantageous, resulting in a clear orientation toward the gradient and avoiding strategies, respectively. Being sessile organisms, fungi use chemical gradients for their orientation in the environment. Integration of this data enables them to successfully explore nutrient sources, identify probable plant or animal hosts, and to communicate during sexual reproduction or early colony development. We have developed a 3D printed device allowing a highly standardized, rapid and low-cost investigation of chemotropic growth processes in fungi. Since the 3D printed device is placed on a microscope slide, detailed microscopic investigations and documentation of the chemotropic process is possible. Using this device, we provide evidence that germlings derived from oval conidia of the hemibiotrophic plant pathogen Colletotrichum graminicola can sense gradients of glucose and reorient their growth toward the nutrient source. We describe in detail the method establishment, probable pitfalls, and provide the original program files for 3D printing to enable broad application of the 3D device in basic, agricultural, medical, and applied fungal science.

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Section: Choice Of Chemoattractant Signalmentioning

confidence: 99%

Section: Preparation Of the C Graminicola Inoculummentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A 3D Printed Device for Easy and Reliable Quantification of Fungal Chemotropic Growth

2020

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“…Apesar de muito semelhantes morfologicamente, estas duas espécies são isoladas reprodutivamente (Vaillancourt & Hanau, 1992). Conídios falcados de C. graminicola, semelhantes àqueles de C. sublineola, também são uninucleados enquanto que conídios ovais Research, Society and Development, v. 9, n. 11, e78491110211, 2020 (CC BY 4.0) | ISSN 2525-3409 | DOI: http://dx.doi.org/10.33448/rsd-v9i11.10211 apresentam de um a três núcleos (Panaccione et al, 1989;Nordzieke et al, 2019). Nishihara (1975) reportou que conídios ovais de C. graminicola são produzidos apenas em cultura líquida, porém Panaccione et al (1989) Os resultados deste estudo demonstraram que o processo de infecção dos conídios ovais em folhas de sorgo é semelhante ao dos falcados, com pequenas variações.…”

Section: Discussionunclassified

Citologia comparativa do processo de infecção do conídio falcado e oval de Colletotrichum sublineola em cultivar de sorgo suscetível e resistente

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et al. 2020

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O modo de infecção primária de Colletotrichum sublineola, agente causal da antracnose em sorgo, ocorre através de conídios falcados produzidos terminalmente em conidióforos nos acérvulos. Um tipo secundário de conídio, o conídio oval, de menor tamanho e ontogenia distinta dos falcados é também produzido por este patógeno. O objetivo deste estudo foi comparar, por meio de microscopia de luz, o processo de infecção entre estes dois tipos de conídios, os ovais e falcados de C. sublineola, em cultivares de sorgo resistente e suscetível à antracnose. Folhas destacadas de 25 dias de idade da BR009 (suscetível) e SC283 (resistente) foram inoculadas com ambos os conídios do isolado patogênico 204.01 e o processo de infecção foi acompanhado por microscopia ótica de 2 a 168 h após a inoculação (AI). Ambos os conídios germinaram 2 h AI e transcorridas 4 h AI, independentemente da cultivar, apressórios arredondados com superfície lisa foram formados diretamente dos conídios falcados, enquanto que apressórios ligeiramente irregulares foram formados na ponta dos tubos germinativos emitidos pelos conídios ovais. Na cultivar suscetível, os apressórios foram formados em maior frequência pelos conídios falcados, enquanto que na cultivar resistente estas estruturas foram formadas em maior frequência pelos conídios ovais. Poros de penetração e hifas de infecção foram visualizados em todos os materiais inoculados com ambos os conídios. Sintomas da doença foram visualizados primeiramente no material inoculado com conídios falcados e foi superior na cultivar suscetível. Lesões com acérvulos contendo conídios falcados foram visualizadas em todos os tratamentos fechando assim o ciclo de infecção.

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Nutritional factors modulating plant and fruit susceptibility to pathogens: BARD workshop, Haifa, Israel, February 25–26, 2018

et al. 2020

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The molecular dialog between fungal pathogens and their plant hosts is governed by signals from the plant, secreted pathogen effectors and enzymes, and the plant immune system. There is an increasing awareness that nutritional factors are also central to fungalplant interactions. Nutritional factors include carbon and nitrogen metabolism, local pH and redox state, and manipulation of host metabolism by secreted pathogen effectors. A diverse combination of approaches from genetics, biochemistry and fungal and plant cell biology addresses these questions, and a workshop whose abstracts accompany this note was held in 2018 to bring these together. Questions were asked about how the lifestyles and nutritional strategies of eukaryotic

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Specialized infection strategies of falcate and oval conidia of Colletotrichum graminicola

Cited by 16 publications

References 63 publications

A 3D Printed Device for Easy and Reliable Quantification of Fungal Chemotropic Growth

A 3D Printed Device for Easy and Reliable Quantification of Fungal Chemotropic Growth

Citologia comparativa do processo de infecção do conídio falcado e oval de Colletotrichum sublineola em cultivar de sorgo suscetível e resistente

Nutritional factors modulating plant and fruit susceptibility to pathogens: BARD workshop, Haifa, Israel, February 25–26, 2018

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