Specialized cell surface structures in cellulolytic bacteria

Lamed, Raphael; Naimark, Jenny; Morgenstern, Ely; Bayer, Edward A.

doi:10.1128/jb.169.8.3792-3800.1987

Cited by 226 publications

(167 citation statements)

References 28 publications

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“…Cell material from a 50 ml culture grown for 8 h was prepared according to Lamed et al (1987) without ferritin.…”

Section: Methodsmentioning

confidence: 99%

Anaerobranca gottschalkii sp. nov., a novel thermoalkaliphilic bacterium that grows anaerobically at high pH and temperature.

Prowe¹,

Antranikian²

2001

International Journal of Systematic and Evolutionary Microbiology

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A novel thermoalkaliphilic, obligately anaerobic bacterium was isolated from a humid soil sample of a hot inlet of Lake Bogoria, Kenya. The newly isolated strain grows optimally at pH 9 5 and 50-55 SC and its growth range is pH 6 0-10 5 and 30-65 SC. Unlike the already known thermoalkaliphiles, the strain grows heterotrophically on a variety of mono-and polysaccharides (glucose, ribose, mannose, fructose, sucrose, maltose, starch, pullulan, xylan and cellulose) and on proteinaceous substrates such as yeast extract, peptone and tryptone. No dissimilatory sulfate reduction was observed, whereas thiosulfate was found to enhance growth when glucose or starch were used as substrates. Under optimal conditions, the doubling time is 48 min. Sodium ions are necessary for growth, with an optimal concentration of 230 mM (1 % NaCl, w/v) at pH 9 5. The rod-shaped cells are motile in the exponential growth phase under optimal growth conditions. Despite the Gram-negative staining and negative KOH assay, the strain is a Gram-positive organism, having an atypically thin cell wall. A sheath-like structure occurs at the cell separation area and parts of a surface layer-like structure were also observed. Based on physiological properties and molecular biological analysis, the strain falls within the radiation of the clostridia and represents a new species of Anaerobranca within the Clostridium/Bacillus subphylum of the Gram-positive bacteria. Strain LBS3 T (l DSM 13577 T ) is named Anaerobranca gottschalkii sp. nov. and is designated as the type strain.

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“…Cell material from a 50 ml culture grown for 8 h was prepared according to Lamed et al (1987) without ferritin.…”

Section: Methodsmentioning

confidence: 99%

Anaerobranca gottschalkii sp. nov., a novel thermoalkaliphilic bacterium that grows anaerobically at high pH and temperature.

Prowe¹,

Antranikian²

2001

International Journal of Systematic and Evolutionary Microbiology

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“…The detection of any cellulase or xylanase activity in the supernatants of each of the CBP experiments was achieved using the Congo red staining method [22,23].…”

Section: Semi-quantitative Assay For Evaluation Of the Secreted Cellumentioning

confidence: 99%

Bioethanol Production from Brewers Spent Grains Using a Fungal Consolidated Bioprocessing (CBP) Approach

et al. 2016

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Production of bioethanol from brewers spent grains (BSG) using consolidated bioprocessing (CBP) is reported. Each CBP system consists of a primary filamentous fungal species, which secretes the enzymes required to deconstruct biomass, paired with a secondary yeast species to ferment liberated sugars to ethanol. Interestingly, although several pairings of fungi were investigated, the sake fermentation system (A. oryzae and S. cerevisiae NCYC479) was found to yield the highest concentrations of ethanol (37 g/L of ethanol within 10 days). On this basis, 1 t of BSG (dry weight) would yield 94 kg of ethanol using 36 hL of water in the process. QRT-PCR analysis of selected carbohydrate degrading (CAZy) genes expressed by A. oryzae in the BSG sake system showed that hemicellulose was deconstructed first, followed by cellulose. One drawback of the CBP approach is lower ethanol productivity rates; however, it requires low energy and water inputs, and hence is worthy of further investigation and optimisation.

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“…The mesophilic anaerobic bacterium Acetivibrio cellulolyticus produces a highly efficient cellulosome capable of hydrolyzing a range of cellulosic materials, including crystalline cellulose, with a higher activity than that of Aspergillus niger and Trichoderma viride systems (7,8). Initial sequencing of an A. cellulolyticus gene cluster identified four tandem scaffoldin genes (scaA, scaB, scaC, and scaD (9,10)).…”

mentioning

confidence: 99%

Cell-surface Attachment of Bacterial Multienzyme Complexes Involves Highly Dynamic Protein-Protein Anchors

Cameron¹,

Najmudin²,

Alves³

et al. 2015

Journal of Biological Chemistry

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Background: Cohesin-dockerin interactions support the binding of multienzyme complexes (cellulosomes) onto the cell surface. Results: The structures of novel Coh-Doc complexes reveal a dual binding mode. Conclusion: A dual binding mode is present in Coh-Doc interactions supporting cell-surface attachment. Significance: The dual binding mode introduces flexibility into highly populated multienzyme complexes attached to the cell surface.

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Specialized cell surface structures in cellulolytic bacteria

Cited by 226 publications

References 28 publications

Anaerobranca gottschalkii sp. nov., a novel thermoalkaliphilic bacterium that grows anaerobically at high pH and temperature.

Anaerobranca gottschalkii sp. nov., a novel thermoalkaliphilic bacterium that grows anaerobically at high pH and temperature.

Bioethanol Production from Brewers Spent Grains Using a Fungal Consolidated Bioprocessing (CBP) Approach

Cell-surface Attachment of Bacterial Multienzyme Complexes Involves Highly Dynamic Protein-Protein Anchors

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