Special issue in quantitative mass spectrometric proteomics

Barnouin, Karin

doi:10.1007/s00726-012-1350-7

Cited by 5 publications

(5 citation statements)

References 15 publications

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“…The majority of these differences are regulated by salt through altered gene expression levels 2 , 10 . Proteomics-based technologies are powerful tools for studying protein abundance 17 , 30 . We previously identified several salt-responsive proteins in M. sativa and M. truncatula roots using two-dimensional gel electrophoresis 31 .…”

Section: Introductionmentioning

confidence: 99%

Quantitative proteomic analysis using iTRAQ to identify salt-responsive proteins during the germination stage of two Medicago species

Long

Gao

Sun

et al. 2018

Sci Rep

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Salt stress is one of the primary abiotic stresses responsible for decreasing crop yields worldwide. Germinating seeds can be greatly influenced by saline conditions. In this study, the physiological and phenotypic changes induced by salt treatments (10–50 mM NaCl and Na2SO4 mixtures) were analysed for Zhongmu-3 (Medicago sativa) and R108 (Medicago truncatula) seedlings. Our observations indicated that Zhongmu-3 was more salt-tolerant than R108. To characterize the protein expression profiles of these two Medicago species in response to salt stress, an iTRAQ-based quantitative proteomic analysis was applied to examine salt-responsive proteins. We identified 254 differentially changed salt-responsive proteins. Compared with control levels, the abundance of 121 proteins increased and 44 proteins decreased in salt-treated Zhongmu-3 seedlings, while 119 proteins increased and 18 proteins decreased in R108 seedlings. Moreover, 48 differentially changed proteins were common to Zhongmu-3 and R108 seedlings. A subsequent functional annotation indicated these proteins influenced diverse processes, such as catalytic activity, binding, and antioxidant activity. Furthermore, the corresponding transcript levels of 15 differentially changed proteins were quantified by qRT-PCR. The data presented herein provide new insights into salt-responsive proteins, with potential implications for enhancing the salt tolerance of Medicago species.

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Section: Introductionmentioning

confidence: 99%

Quantitative proteomic analysis using iTRAQ to identify salt-responsive proteins during the germination stage of two Medicago species

Long

Gao

Sun

et al. 2018

Sci Rep

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“…In recent years, new mass spectrometry-based proteomic techniques, such as isobaric tags for relative and absolute quantitation (iTRAQ) and Isotope-coded affinity tags (ICAT), have been applied for proteomic analysis. Among them, iTRAQ is the most popular technique in plant quantitative proteomics1617. This technique is based on tagging the N-terminus of peptides generated from tryptic protein digests, which overcomes some limitations of gel-based techniques and also improves the throughput of proteomic studies.…”

mentioning

confidence: 99%

“…This technique is based on tagging the N-terminus of peptides generated from tryptic protein digests, which overcomes some limitations of gel-based techniques and also improves the throughput of proteomic studies. Furthermore, this technique has a high degree of sensitivity, and the amine specific isobaric reagents permit the identification and quantitation of up to 8 different samples simultaneously1617. Thus, in this paper, we employed iTRAQ and liquid chromatography-tandem mass spectrometry (LC-MS/MS) to further understand the molecular mechanisms underlying high temperature stress in Pyropia and reveal the global proteomic response of the Z-61 stains of P. haitanensis under high temperature stress.…”

mentioning

confidence: 99%

Differential Proteomic Analysis by iTRAQ Reveals the Mechanism of Pyropia haitanensis Responding to High Temperature Stress

Shi

Chen

et al. 2017

Sci Rep

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Global warming increases sea temperature and leads to high temperature stress, which affects the yield and quality of Pyropia haitanensis. To understand the molecular mechanisms underlying high temperature stress in a high temperature tolerance strain Z-61, the iTRAQ technique was employed to reveal the global proteomic response of Z-61 under different durations of high temperature stress. We identified 151 differentially expressed proteins and classified them into 11 functional categories. The 4 major categories of these are protein synthesis and degradation, photosynthesis, defense response, and energy and carbohydrate metabolism. These findings indicated that photosynthesis, protein synthesis, and secondary metabolism are inhibited by heat to limit damage to a repairable level. As time progresses, misfolded proteins and ROS accumulate and lead to the up-regulation of molecular chaperones, proteases, and antioxidant systems. Furthermore, to cope with cells injured by heat, PCD works to remove them. Additionally, sulfur assimilation and cytoskeletons play essential roles in maintaining cellular and redox homeostasis. These processes are based on signal transduction in the phosphoinositide pathway and multiple ways to supply energy. Conclusively, Z-61 establishes a new steady-state balance of metabolic processes and survives under higher temperature stress.

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“…This technique overcomes some limitations of gel-based techniques and expands the throughput of proteomic studies. In addition, the amine specific isobaric reagents permit the identification and quantitation of up to 8 different samples simultaneously [ 41 , 42 ]. Recently, iTRAQ has been used in insect quantitative proteomics, such as the mountain pine beetle, Dendroctonus ponderosae (Hopkins) (Coleoptera: Scolytidae) [ 43 ], migratory locust [ 44 ], termite [ 45 ], Aphidius gifuensis (Ashmead) (Hymenoptera: Braconidae) [ 46 ] and pea aphid, Acyrthosiphon pisum (Harris) (Hemiptera: Aphididae) [ 47 ].…”

Section: Introductionmentioning

confidence: 99%

Differential Proteomics Analysis Unraveled Mechanisms of Arma chinensis Responding to Improved Artificial Diet

Zou

Coudron²,

Wu³

et al. 2022

Insects

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The development of artificial diets could considerably simplify and reduce the cost of mass rearing of natural enemies compared to conventional rearing methods. However, improvement of artificial diets can be tedious, convoluted and often uncertain. For accelerating diet development, a better method that can offer informative feedback to target deficiencies in diet improvement is required. Our previous research demonstrated several biological characteristics were diminished in the insect predator, Arma chinensis Fallou, fed on an artificial diet formulated with the aid of transcriptomic methods compared to the Chinese oak silk moth pupae. The present study reports differential proteomic analysis by iTRAQ-PRM, which unravels the molecular mechanism of A. chinensis responding to improvements in the artificial diet. Our study provides multivariate proteomic data and provides comprehensive sequence information in studying A. chinensis. Further, the physiological roles of the differentially expressed proteins and pathways enable us to explain several biological differences between natural prey-fed and improved diet-fed A. chinensis, and subsequent proposed reformulation optimizations to artificial diets.

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Special issue in quantitative mass spectrometric proteomics

Cited by 5 publications

References 15 publications

Quantitative proteomic analysis using iTRAQ to identify salt-responsive proteins during the germination stage of two Medicago species

Quantitative proteomic analysis using iTRAQ to identify salt-responsive proteins during the germination stage of two Medicago species

Differential Proteomic Analysis by iTRAQ Reveals the Mechanism of Pyropia haitanensis Responding to High Temperature Stress

Differential Proteomics Analysis Unraveled Mechanisms of Arma chinensis Responding to Improved Artificial Diet

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