Special issue: Advanced applications of plasmas in Life Sciences 2020

Favia

Fracassi

et al. 2021

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Plasma-treated water solutions (PTWS) allow the delivery of reactive oxygen and nitrogen species (RONS) to cells and tissues for different purposes. The mechanism of RONS formation has been clearly modelled in simple liquids like water, by assuming a plasma-driven process independent from the liquid. PTWS for biological experiments, however, are often produced from solutions of complex composition, where the formation mechanism of RONS is far from being understood. In this paper, we describe how water, phosphate-buffered saline solution and two cell culture media were plasma-treated in different conditions to demonstrate how the different composition of the liquids affects the formation of stable RONS (H 2 O 2 and NO 2 − ) in the resulting PTWS, especially when aromatic organic molecules are present.

Section: Introductionmentioning

confidence: 99%

The active role of organic molecules in the formation of long‐lived reactive oxygen and nitrogen species in plasma‐treated water solutions

Favia

Fracassi

et al. 2021

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“…In the last decades, cold atmospheric plasmas (CAPs) have exhibited a huge potential for biological and clinical applications [1][2][3] such as disinfection or [4][5][6] wound healing [7] and as innovative anticancer agents. [8][9][10][11] The efficacy of CAP in these fields primarily derives from their ability to deliver gaseous blends of reactive oxygen and nitrogen species (RONS), well-known as redox modulators and oxidative stress inducers [1,8,12] in redox biology. Among them, hydrogen peroxide (H 2 O 2 ), nitrate (NO 3 − ) nitrite ions (NO 2 − ) are the most stable RONS species, above all when they are transferred to liquid samples.…”

Section: Introductionmentioning

confidence: 99%

The effect of different cold atmospheric plasma sources and treatment modalities on the generation of reactive oxygen and nitrogen species in water

Armenise

Cosmai

et al. 2022

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Reactive oxygen and nitrogen species (RONS) can be generated in liquids by different cold plasma processes. These species can influence cell biology and pathological states, and trigger wound healing, disinfection, and cancer treatments. The optimization of plasma sources' configurations and experimental conditions is fundamental to dose-produced RONS in liquids. This research is a case study comparing RONS generation (H 2 O 2 and NO 2 − ) in water, after exposure to two different atmospheric pressure cold dielectric barrier discharge (DBD) plasma sources: a plasma jet-DBD and a planar-DBD operating in contact with the liquid and in remote, respectively. The data demonstrate that the reactor geometry and the treatment modality strongly impact the generation of the active species in water.

“…[1,2] Surface modification processes for biomaterials have been the most popular outcomes of NTP in the biomedical area for about 50 years. [1,3] In the last one-two decades, though, NTP are being continuously proposed for newer uses in Life Sciences, namely, for the decontamination of wounds, [4] for cancer treatments, [5,6] and for activating water and seeds to improve germination and harvest. [7] The NTP processes investigated in this direction are ignited at atmospheric pressure (AP), generally in air or other gases including inert ones, in close proximity to living matter or water-based liquids.…”

Section: Introductionmentioning

confidence: 99%

Validation of colorimetric assays for hydrogen peroxide, nitrate and nitrite ions in complex plasma‐treated water solutions

Favia

Fracassi

et al. 2021

Self Cite

Liquids treated with cold plasma emerged as ‘redox drugs’ in biomedicine, as sources of reactive oxygen and nitrogen species targeting cellular functions, including wound healing and cancer progression. The use of cell culture media as starting liquid, however, challenges the identification of plasma‐generated chemistry, limited by the presence of many reactive species and organic compounds. Available detection methods need, therefore, to be confirmed in these liquids to avoid inaccurate results. In this research, robustness, linearity, accuracy and specificity of three colorimetric assays are investigated to detect H2O2, NO2− and NO3−, predominant plasma‐induced products. The results clearly highlight the presence of some factors affecting the detection in cell culture media like high concentrations of chlorides found interfering with the detection of NO3− in the medium.