Special dyeing, histochemistry, immunohistochemistry and ultrastructure: A study of mast cells/eosinophilic granules cells (MCs/EGC) from  Centropomus parallelus  intestine

Silva, Wémeson F. da; Simões, Manuel de Jesus; Gutierre, Robson Campos; Egami, Mizue Imoto; Santos, Antenor Aguiar; Antoniazzi, Marta Maria; Sasso, Gisela Rodrigues da Silva; Ranzani-Paiva, María José Tavares

doi:10.1016/j.fsi.2016.11.022

Cited by 21 publications

(15 citation statements)

References 52 publications

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“…Records on contents of granules of fish MCs revealed that they have a panel of inflammatory mediators (Da Silva et al., ; Dezfuli, Lui, Giovinazzo, Boldrini, & Giari, ; Galindo‐Villegas et al., ; Silphaduang & Noga, ). MCs degranulation was observed after inoculation of Aeromonas salmonicida exotoxins (Ellis, ; Vallejo & Ellis, ), although degranulation of MCs was induced ex vivo by use of compound 48/80 on intestinal tissue of rainbow trout (Manera, Giammarino, Borreca, Giari, & Dezfuli, ).…”

Section: Discussionmentioning

confidence: 99%

Histopathological and ultrastructural assessment of two mugilid species infected with myxozoans and helminths

Dezfuli

Castaldelli

Giari

2017

Journal of Fish Diseases

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The histopathology and ultrastructure of the intestine of mullets, Liza ramada and Liza saliens, from Comacchio lagoons (northern Italy) naturally infected with myxozoans and helminths were investigated and described. Sixty-two (80.5%) of 77 mullets harboured one or more of the following parasites species: Myxobolus mugchelo (Myxozoa), Neoechinorhynchus agilis (Acanthocephala), Haplosplanchnus pachysomus and Dicrogaster contractus (Digenea). Co-occurrence of helminths with myxozoans was common. The main damage caused by digeneans was destruction of the mucosal epithelium of the villi, necrosis and degeneration of intestinal epithelial cells. More severe intestinal damage was caused by acanthocephalans which reach the submucosa layer with their proboscis. At the site of helminths infection, several mast cells (MCs), rodlet cells (RCs), mucous cells and few neutrophils and macrophages were observed in the epithelium. RCs and mucous cells exhibited discharge activity in close vicinity to the worm's tegument. M. mugchelo conspicuous plasmodia were encysted mainly in muscle and submucosa layers of the intestine. Indeed, spores of M. mugchelo were documented within the epithelial cells of host intestine and in proximity to MCs. Degranulation of the MCs near the myxozoans was very frequent.

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Section: Discussionmentioning

confidence: 99%

Histopathological and ultrastructural assessment of two mugilid species infected with myxozoans and helminths

Dezfuli

Castaldelli

Giari

2017

Journal of Fish Diseases

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“…The change in the frequency of intestinal eosinophils can occur through changes in the differentiation of eosinophils in the bone marrow, leading to changes in circulating association, migration of these cells from the circulation to a target tissue, and changes in survival longevity within the tissue (SVENSSON-FREJ, 2011). In acute exposure, eosinophils, a kind of multifunctional leukocytes with an important role in parasitic infections and allergic inflammations, were found in a smaller number in the higher concentrations of MnCl 2 tested, characterizing an abnormal condition, since these cells appear in greater quantity in the fish gills and intestines, playing an important role in the recovery of lesions (SFACTERIA et al, 2015;DA SILVA et al, 2017). Thus, the reduction in the frequency of these cells can lead to problems in intestinal immunity (CHEN et al, 2015).…”

Section: Introduction 2 Materials and Methodsmentioning

confidence: 99%

Evaluation of intestinal histological damage in zebrafish exposed to environmentally relevant concentrations of manganese

Rodrigues

Souza

Silva

et al. 2018

CeN

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Manganese is an essential element for many physiological processes. However, in prolonged exposures or at high concentrations it may present toxicity to several organs, which has made it an object of study in pharmacological, behavioral, neurological and environmental research in different organisms. Thus, it was aimed to evaluate the damage caused by acute and chronic exposures to this element, through the histopathological analysis of the zebrafish (Danio rerio) intestine. Samples were embedded in paraffin for further processing in rotating microtome, and the slides were stained with Alcian Blue and hematoxylin for observation and analysis under light microscopy. The results demonstrated the potential of the metal to cause histological changes in this organ, such as fusion of villi (in 0.5 and 4.0 mg L-1), and to increase by more than 50% the number of goblet cells (in 8.0 and 16 mg L-1) in the region analyzed, mainly in the highest concentrations of Manganese Chloride and in chronic exposures, characterizing a possible inflammation of the intestinal epithelium. Thus, the histological analysis of intestines demonstrated to be a reliable alternative for the toxicological evaluation. It is important to monitor the concentrations of this and other metals in the water bodies, given their toxicity.

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“…Tryptase is a serine protease produced and stored in mast cell granules (McNeil et al ., 2007). Tryptase has been reported in gill mast cells of zebrafish (Dobson et al ., 2008), and in mucosal mast cells of the intestine of the fat snook, Centropomus parallelus Poey, 1860 (da Silva et al ., 2017), suggesting that the occurrence of tryptase is supportive in host tissue repair processes (Duchesne et al ., 2011). In the present parasitized liver samples of blue whiting, numerous macrophages and MAs were noticed, and they were positive to the antibody anti-mast cell tryptase.…”

Section: Discussionmentioning

confidence: 99%

Immunohistopathological response against anisakid nematode larvae and a coccidian in Micromesistius poutassou from NE Atlantic waters

et al. 2021

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A survey on Anisakis simplex (sensu stricto (s.s.)) from blue whiting, Micromesistius poutassou, in the north-eastern Atlantic Ocean revealed the occurrence of high infection levels of third larval stages in visceral organs and flesh. Larvae were genetically identified with a multilocus approach as A. simplex (s.s.). Histochemical, immunohistochemical and ultrastructural observations were conducted on 30 M. poutassou specimens. Gonads, pyloric caeca and flesh harboured encapsulated larvae of A. simplex (s.s.) but no intense host reaction was encountered around the parasite in the above organs. In the liver, the most infected organ, the larvae co-occurred with the coccidian Goussia sp. Within the granuloma around the A. simplex (s.s.) larvae, two concentric layers were recognized, an inner mostly comprising electron-dense epithelioid cells and an outer layer made of less electron-dense epithelioid cells. Macrophages and macrophage aggregates (MAs) were abundant out of the granulomas, scattered in parenchyma, and inside the MAs, the presence of engulfed Goussia sp. was frequent. In liver tissue co-infected with Goussia sp. and A. simplex (s.s.), hepatocytes showed cytoplasmic rarefaction and acute cell swelling. Results suggest that the host-induced encapsulation of A. simplex (s.s.) larvae is a strategic compromise to minimize collateral tissue damage around the larval infection sites, to facilitate the survival of both parasite and host.

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Special dyeing, histochemistry, immunohistochemistry and ultrastructure: A study of mast cells/eosinophilic granules cells (MCs/EGC) from Centropomus parallelus intestine

Cited by 21 publications

References 52 publications

Histopathological and ultrastructural assessment of two mugilid species infected with myxozoans and helminths

Histopathological and ultrastructural assessment of two mugilid species infected with myxozoans and helminths

Evaluation of intestinal histological damage in zebrafish exposed to environmentally relevant concentrations of manganese

Immunohistopathological response against anisakid nematode larvae and a coccidian in Micromesistius poutassou from NE Atlantic waters

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