Special Considerations with Floor of Mouth and Tongue Cancer

Arosarena, Oneida A.; Madsen, Matthew J.; Haug, Richard H.

doi:10.1016/j.coms.2006.06.005

Cited by 2 publications

(2 citation statements)

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“…Oral cancer (OC) is the sixth most common cancer worldwide and has one of the highest mortality rates, due to it being largely asymptomatic until the latter stages of the disease. Cancers of the oral cavity include cancers that occur in the tongue, floor of the mouth, buccal mucosa, alveolus, retromolar trigone, gingival, hard palate, and lips (1,2). A total of 90% of OCs are squamous cell carcinomas (SCC) (3).…”

Section: Introductionmentioning

confidence: 99%

Induction of apoptosis in oral squamous carcinoma cells by pyrrolo-1,5-benzoxazepines

O’Callaghan

Palagano

Butini

et al. 2015

Molecular Medicine Reports

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Oral cancer (OC) is a largely asymptomatic disease, resulting in one of the highest mortality rates of any cancer. OC is currently ranked as the sixth most common cancer in the world, according to a recent World Health Organization analysis, and its prevalence is increasing, both in western and developing regions. Depending on the stage of OC, treatment strategies include surgery, radiation therapy and chemotherapy, or a combination thereof. As with numerous other types of cancer, resistance to conventional chemotherapeutic drugs is increasing in oral squamous cell carcinoma (OSCC). The present study aimed to investigate the use of a novel group of compounds, the pyrrolo‑1,5‑benzoxazepines (PBOXs), as a therapeutic alternative for the treatment of OC. PBOXs are microtubule‑targeting agents that are able to induce apoptosis in numerous cancer cell types, thereby preventing tumour cell proliferation. Ca9.22 gingival and TR146 buccal cell lines were used as models for OSSC. Cell viability and proliferation in the presence of two PBOXs: PBOX‑6 and PBOX‑15, was monitored using an AlamarBlueTM assay. Flow cytometric analysis of propidium iodide‑stained cells was used to determine the DNA content, and therefore the percentage of cells in each phase of the cell cycle. Microtubule disruption was determined by indirect immunofluorescence staining. Changes in protein expression and degradation were determined by western blotting. The results of the present study indicated that both PBOX‑6 and ‑15 were able to induce apoptotic cell death by disrupting the microtubule network in both cell lines. The EC50 values were subsequently calculated for both PBOX‑6 and ‑15, and PBOX‑15 was shown to possess a higher potency. Both compounds displayed anti‑proliferative effects mediated through sustained G2/M arrest accompanied by tubulin disruption, and a decrease in DNA repair protein poly (ADP ribose) polymerase expression. These findings suggest that PBOXs may prove useful, either alone or in combination with other agents, in the treatment of chemotherapeutic resistant OSCC.

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Section: Introductionmentioning

confidence: 99%

Induction of apoptosis in oral squamous carcinoma cells by pyrrolo-1,5-benzoxazepines

O’Callaghan

Palagano

Butini

et al. 2015

Molecular Medicine Reports

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“…O aparecimento de metástase em linfonodos cervicais ocorrem em cerca de 33% dos casos (Hosal et al, 2000;Kuriakose;Trivedi, 2009). O comportamento agressivo dos tumores prim rios de CECP, est relacionado alta disseminação e a propensão a met stases em linfonodos cervicais (Arosarena et al, 2006;Lim et al, 2006).…”

Section: Revisão De Literaturaunclassified

Identificação de microRNAs circulantes e avaliação de sua contribuição como marcador de metástases em carcinoma epidermoide de cabeça e pescoço

Andreghetto¹

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MicroRNAs are small non-coding RNA molecules with roles in gene regulation and implications in cancer initiation and progression. Cell-free microRNAs detected in body fluids are of clinical interest due to their possible application as biomarkers. The stability of microRNAs in plasma has been demonstrated and it seems that alterations in cancer tissue may be detected in plasma from patients. Despite the fact that their reliable quantification depends on several technical parameters there is great clinical interest in this approach due to its simplicity and low risk to the patient. Oral squamous cell carcinoma is one of the most common cancer types worldwide. Tobacco and alcohol consumption are the most important risk factors and survival rates are about 50% in 5 years. The aim of this study was to establish reliable parameters for the detection of microRNAs in plasma and to identify plasma microRNAs associated with squamous cell carcinomas of the oral cavity and oropharynx as well as with the presence of cervical lymph node metastases. With this purpose we evaluated the expression of 179 microRNAs in plasma from 45 patients (28 presenting cervical lymph node metastases at diagnosis and 17 with no lymph node metastasis) and 15 healthy controls. Samples were matched according to age, sex, drinking and smoking habits. We identified microRNAs mostly expressed in tumor samples, some of which already described in oral squamous cell carcinoma, such as miR-210, and microRNAs poorly studied, such as miR-573. Comparisons between oral and oropharynx carcinomas yielded several differences, in accordance with molecular differences known between these cancer types. MicroRNAs possibly involved in the metastatic process were identified between plasma samples from individuals presenting lymph node metastasis at diagnosis and plasma from patients who were metastasis-free. Among these molecules, miR-192 and miR-574 were identified as more expressed in plasma from individuals with metastatic oropharynx squamous cell carcinoma. We conclude that plasma microRNAs may indicate molecular characteristics of the tumor and that its differential detection in association with specific clinical conditions or sub-sites may be considered as a potential tool to help in the diagnosis and prognosis assessment of head and neck squamous cell carcinoma.

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Special Considerations with Floor of Mouth and Tongue Cancer

Cited by 2 publications

References 93 publications

Induction of apoptosis in oral squamous carcinoma cells by pyrrolo-1,5-benzoxazepines

Induction of apoptosis in oral squamous carcinoma cells by pyrrolo-1,5-benzoxazepines

Identificação de microRNAs circulantes e avaliação de sua contribuição como marcador de metástases em carcinoma epidermoide de cabeça e pescoço

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