Spatiotemporal Expression Patterns and Antibody Reactivity of Taeniidae Endophilin B1

Abstract

h Larval Taeniidae, such as metacestodes of Taenia solium, Echinococcus granulosus, and Echinococcus multilocularis, produce chronic and fatal helminthic diseases. Proper identification of these zoonotic cestodiases is often challenging and is hampered in some clinical settings. Endophilin B1 plays critical roles in the maintenance of membrane contours and endocytosis. We isolated proteins homologous to endophilin B1 from T. solium, Taenia saginata, and Taenia asiatica. The three Taeniidae endophilin B1 proteins shared 92.9 to 96.6% sequence identity. They harbored a Bin1/amphiphysin/Rvs (BAR) domain and residues for a dimeric interface but lacked a SRC homology 3 (SH3) domain. Endophilin B1 showed a unique immunological profile and was abundantly expressed in the tegumental syncytium of Taeniidae

Extracellular vesicles from Echinococcus granulosus larval stage: Isolation, characterization and uptake by dendritic cells

“…In our proteomics data, we found 14-3-3 proteins which have been reported to provide 97% protection against E . multilocularis challenge infection in rodent and the production of a specific humoral response in rhesus macaque models, respectively [86, 87], the endophilin B1, which is highly expressed in the tegument of Taeniidae metacestodes and responsible for a strong immune recognition in sera from patients with cystic and alveolar echinococcosis and chronic neurocysticercosis [88], antigen 5, an immuno-dominant serine-protease with heparan sulphate proteoglycan- and calcium-binding sites, and antigen P-29 immunologically related to antigen 5 used as a marker for post-surgery surveillance of cystic echinococcosis patients [89, 90]. Antigen 5 is highly immunogenic in human infections although its highly cross-reactive glycan moieties may involve a parasite evasion mechanism [91–95].…”

Evaluation of the diagnostic value of the immunoblotting and ELISA tests using recombinant Em18 antigen in human alveolar echinococcosis from Xingjiang China

“…Alveolar echinococcosis (AE) causes high chronic morbidity and mortality in Central Asia, European countries, North/Latin America and northwest China (15). One of the clinical features of tissue-invasive larval cestodiases is slow progression with minimal symptoms and signs, unless infected parasites provoke acute symptoms (16).…”

Comparison of Echinococcus multilocularis and Echinococcus granulosus hydatid fluid proteome provides molecular strategies for specialized host-parasite interactions

“…For example, we observed membrane-sensing endophilin B1 (previously known as P29) and signal-related 14-3-3 proteins only in EgHF (Figure 4 and Table 2 ). However, the authentic activity of those molecules was confirmed in E. multilocularis [ 39 , 40 ]. This apparent discrepancy could suggest that expression levels of these molecules in E. multilocularis metacestode might not be sufficiently high to have been detectable under our experimental conditions.…”