Comparison of Echinococcus multilocularis and Echinococcus granulosus hydatid fluid proteome provides molecular strategies for specialized host-parasite interactions

Abstract

Alveolar and cystic echinococcoses, caused by the metacestodes of Echinococcus multilocularis and E. granulosus, are prevalent in several regions and invoke deleterious zoonotic helminthiases. Hydatid fluid (HF), which contains proteinaceous and non-proteinaceous secretions of the parasite- and host-derived components, critically affects the host-parasite interplay and disease progression. We conducted HF proteome profiling of fully mature E. multilocularis vesicle (nine months postinfection) and E. granulosus cyst (stage 2). We identified 120 and 153 proteins, respectively, in each fluid. Fifty-six and 84 proteins represented distinct species; 44 and 66 were parasites, and 12 and 18 were host-derived proteins. The five major parasite protein populations, which included antigen B isoforms, metabolic enzymes, proteases and inhibitors, extracellular matrix molecules (ECMs), and developmental proteins, were abundantly distributed in both fluids and also exclusively in one sample or the other. Carbohydrate-metabolizing enzymes were enriched in E. granulosus HF. In the E. multilocularis HF, proteins that constitute ECMs, which might facilitate adhesion and cytogenesis, were highly expressed. Those molecules had physical and functional relationships along with their biochemical properties through protein-protein interaction networks. Twelve host-derived proteins were largely segregated to serum components. The major proteins commonly and uniquely detected in these HFs and their symbiotic interactome relationships might reflect their biological roles in similar but distinct modes of maturation, invasion, and the longevity of the parasites in the hosts.

The secreted protein signature of hydatid fluid from pulmonary cystic echinococcosis

“…AgB8/1 has been reported as the most abundant subunit in E. granulosus AgB oligomer [36], and it is the AgB subunit consistently identified by MS based analysis of HF [8,14,37]. However, the detection and the abundance of AgB8/2 to 5 in HF is rather variable [8,9,14,37]. Also, the AgB subunits representation in HF vary among different Echinococcus species [14,38].…”

“…However, the detection and the abundance of AgB8/2 to 5 in HF is rather variable [8,9,14,37]. Also, the AgB subunits representation in HF vary among different Echinococcus species [14,38]. multilocularis [13,37,39].…”

“…ES proteomic studies included the analysis of different components of E. granulosus cysts [8]; and comparisons among hydatid cyst fluid of E. granulosus cysts from different hosts (sheep, cattle and humans) [9]; and the comparison of HF from two different isolates of Echinococcus multilocularis, the etiological agent of alveolar echinococcosis [13]. Within the genus Echinococcus, interspecies comparisons in proteomic studies have been performed only between E. granulosus and E. multilocularis [14]. From all these studies, it became evident that analyses of the same species infecting different hosts, and different genotypes/species/strains infecting a common host, can provide valuable insights on molecular survival strategies adopted by parasites.…”

Combining proteomics and bioinformatics to explore novel tegumental antigens as vaccine candidates against Echinococcus granulosus infection

“…To obtain a protein-by-protein characterization of PSEx, an extensive in silico study of its identified proteome was performed ( Table 2). Prediction of cellular localization showed a predominance (73%) of cytoplasmic proteins (42 in 57), followed by 11% characterized as mitochondrial 35,39 Abbreviations: aa, amino acid; IP, isoelectric point. ; MW, molecular weight; IP, isoelectric point.…”

Primary pelvic hydatid cyst in an infertile female, A case report

“…2 The parasites can grow in two forms: unilocular cyst or cystic echinococcosis (CE) and multilocular cyst or alveolar echinococcosis (AE). 3 CE is the most common form of disease among nomadic area in which dogs play an important role in habitation. Echinococcus granulosus (E granulosus) can grow in every organ or body tissue and produce small cysts.…”