Spatiotemporal Control of Molecular Cascade Reactions by a Reconfigurable DNA Origami Domino Array

Fan, Sisi; Liu, Yan; Zou, Kexuan; Tian, Zhijin; Dai, Bin; Cui, Daxiang; Zhang, Pengfei; Ke, Yonggang; Song, Jie

doi:10.1002/anie.202116324

Cited by 12 publications

(13 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Section: Nanomaterials Templated By Dna Origamimentioning

confidence: 99%

“…In addition, the structural continuity of the origami between the enzyme linkages affected the catalytic activity. Furthermore, Fan et al designed a reconfigurable DNA origami domino array (DODA) for the dynamic control of molecular cascade reactions . The DODA structure was used to anchor the reactants with nanometer spatial precision (Figure D).…”

Section: Nanomaterials Templated By Dna Origamimentioning

confidence: 99%

See 1 more Smart Citation

Recent Advances in DNA Origami-Engineered Nanomaterials and Applications

et al. 2023

Self Cite

View full text Add to dashboard Cite

DNA nanotechnology is a unique field, where physics, chemistry, biology, mathematics, engineering, and materials science can elegantly converge. Since the original proposal of Nadrian Seeman, significant advances have been achieved in the past four decades. During this glory time, the DNA origami technique developed by Paul Rothemund further pushed the field forward with a vigorous momentum, fostering a plethora of concepts, models, methodologies, and applications that were not thought of before. This review focuses on the recent progress in DNA origami-engineered nanomaterials in the past five years, outlining the exciting achievements as well as the unexplored research avenues. We believe that the spirit and assets that Seeman left for scientists will continue to bring interdisciplinary innovations and useful applications to this field in the next decade.

show abstract

Section: Nanomaterials Templated By Dna Origamimentioning

confidence: 99%

Section: Nanomaterials Templated By Dna Origamimentioning

confidence: 99%

Recent Advances in DNA Origami-Engineered Nanomaterials and Applications

et al. 2023

Self Cite

View full text Add to dashboard Cite

show abstract

“…Nucleic acids are outstanding bricks for constructing ordered nanostructures, [89,90] and these nanostructures, often called DNA origami, can serve as scaffolds for enzyme assembly [91–95] . For example, Hao, Gang, Chen, Niemeyer, and other researchers have designed artful DNA structures to precisely control the position of immobilized enzymes [96–110] . However, most of the DNA‐enzyme complexation systems were manifested in vitro or for cell‐free synthesis, and those that regulate biosynthesis inside cells are relatively rare.…”

Section: Scaffolded Enzyme Assemblymentioning

confidence: 99%

“…[91][92][93][94][95] For example, Hao, Gang, Chen, Niemeyer, and other researchers have designed artful DNA structures to precisely control the position of immobilized enzymes. [96][97][98][99][100][101][102][103][104][105][106][107][108][109][110] However, most of the DNA-enzyme complexation systems were manifested in vitro or for cell-free synthesis, and those that regulate biosynthesis inside cells are relatively rare. Conrado et al reported an outstanding example of DNA-guided assembly of biosynthetic pathways in living cells.…”

Section: Nucleic Acids As Scaffoldsmentioning

confidence: 99%

Synthetic Multienzyme Assemblies for Natural Product Biosynthesis

et al. 2023

View full text Add to dashboard Cite

In nature, enzymes that catalyze sequential reactions are often assembled as clusters or complexes. The formation of multienzyme complexes, or metabolons, brings the enzyme active sites into proximity to promote intermediate transfer, decrease intermediate leakage, and streamline the metabolic flux towards the desired products. We and others have developed synthetic versions of metabolons through various strategies to enhance the catalytic rates for synthesizing valuable chemicals inside microbes. Synthetic multienzyme complexes range from static enzyme nanostructures to dynamic enzyme coacervates. Enzyme complexation optimizes the metabolic fluxes inside microbes, increases the product titer, and supplies the field with high-yield microbe strains that are amenable to large-scale fermentation. Enzyme complexes constructed inside microbial cells can be separated as independent entities and catalyze biosynthetic reactions ex vivo; such a feature gains these complexes another name, "synthetic organelles" -new subcellular entities with independent structures and functions. Still, the field is seeking new strategies to better balance dynamicity and confinement and to achieve finer control of local compartmentalization in the cells, as the natural multienzyme complexes do. Industrial applications of synthetic multienzyme complexes for the large-scale production of valuable chemicals are yet to be realized. This review focuses on synthetic multienzyme complexes that are constructed and function inside microbial cells.

show abstract

“…Tile-based self-assembly methods [ 22 , 23 ] assemble basic motifs into structures through a periodic interaction between the motifs ( Figure 1 a). DNA origami-based self-assembly methods [ 24 , 25 ] utilize many short staple strands to bind a long, single DNA strand and fold it into a certain shape ( Figure 1 b). DNA brick-based self-assembly methods [ 26 , 27 ] combine hundreds of single strands into a designed structure, and the design process is similar to filling a canvas with pixels ( Figure 1 c).…”

Section: Introductionmentioning

confidence: 99%

Active Self-Assembly of Ladder-Shaped DNA Carrier for Drug Delivery

et al. 2023

View full text Add to dashboard Cite

With the advent of nanotechnology, DNA molecules have been transformed from solely genetic information carriers to multifunctional materials, showing a tremendous potential for drug delivery and disease diagnosis. In drug delivery systems, DNA is used as a building material to construct drug carriers through a variety of DNA self-assembly methods, which can integrate multiple functions to complete in vivo and in situ tasks. In this study, ladder-shaped drug carriers are developed for drug delivery on the basis of a DNA nanoladder. We first demonstrate the overall structure of the nanoladder, in which a nick is added into each rung of the nanoladder to endow the nanoladder with the ability to incorporate a drug loading site. The structure is designed to counteract the decrement of stability caused by the nick and investigated in different conditions to gain insight into the properties of the nicked DNA nanoladders. As a proof of concept, we fix the biotin in every other nick as a loading site and assemble the protein (streptavidin) on the loading site to demonstrate the feasibility of the drug-carrying function. The protein can be fixed stably and can be extended to different biological and chemical drugs by altering the drug loading site. We believe this design approach will be a novel addition to the toolbox of DNA nanotechnology, and it will be useful for versatile applications such as in bioimaging, biosensing, and targeted therapy.

show abstract

Spatiotemporal Control of Molecular Cascade Reactions by a Reconfigurable DNA Origami Domino Array

Cited by 12 publications

References 44 publications

Recent Advances in DNA Origami-Engineered Nanomaterials and Applications

Recent Advances in DNA Origami-Engineered Nanomaterials and Applications

Synthetic Multienzyme Assemblies for Natural Product Biosynthesis

Active Self-Assembly of Ladder-Shaped DNA Carrier for Drug Delivery

Contact Info

Product

Resources

About