Spatio-temporal distribution of Grapevine fanleaf virus (GFLV) in grapevine

Krebelj, Anastazija Jež; Čepin, Urška; Ravnikar, Maja; Pompe-Novak, Maruša

doi:10.1007/s10658-015-0600-4

Cited by 16 publications

(8 citation statements)

References 23 publications

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“…In samples Ref9 2/71, LR 5/32, RefDUC 3/8, Ste 4/37 and Zun 5/18, satRNAs could barely be detected at the beginning of the seasons in 2008 and 2011, and could not be detected at all in autumn 2010, even though the same extraction procedure was used for samples collected in spring 2011 and autumn 2010. These results correlate with the seasonal trend of GFLV titres that was reported previously (Jež Krebelj et al ., ). qPCR analyses of phloem samples within the same study also confirmed that reference genes COX and 18S rRNA resulted in constant C q values during the season (data not shown), which means that efficiency of extraction was not dependent on the sampling time period.…”

Section: Discussionmentioning

confidence: 97%

Frequency of occurrence and genetic variability of Grapevine fanleaf virus satellite RNA

et al. 2015

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Little is known about the natural occurrence and genetic variability of nepovirus large satellite RNA (satRNA). This study screened 71 Grapevine fanleaf virus (GFLV) isolates mainly from Slovenia, but also from other countries in Europe and the USA, for the presence of satRNA, using a newly developed RT-PCR assay. GFLV satRNA (satGFLV) was detected in 72% of naturally GFLV-infected grapevines analysed, which is the highest frequency of occurrence of sat-GFLV reported to date. From 39 naturally GFLV-infected grapevines, 122 satGFLV clones were sequenced and compared to publicly available sequences of satGFLVs and the closely related satRNAs from Arabis mosaic virus (satArMVs). Phylogenetic analyses of these satRNAs revealed that their evolution was driven by substitutions, insertions, deletions, recombinations and reassortments between closely related helper viruses. Phylogenetic relationships of the satGFLVs and satArMVs show their separate and subsequent common evolution. Furthermore, the satGFLVs varied in size and showed higher variability at the amino acid level than at the nucleotide level, just as the 2AHP gene of their helper virus. This study shows that satGFLVs are also similar to their helper virus with respect to their quasispecies nature and their transmission route through anthropogenic exchange of propagation material.

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Section: Discussionmentioning

confidence: 97%

Frequency of occurrence and genetic variability of Grapevine fanleaf virus satellite RNA

et al. 2015

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“…S3 ), and at Tf the titre decreased in both cultivars. Krebelj et al 39 reported that in vineyards, the highest GFLV titre was observed at the beginning of the vegetative period in May, and that the virus concentration changed in different grapevine cultivar. Our data agree with their study: the lower GFLV concentration at Tf may be considered a seasonal variation of virus titre, although decrease in viral titre may be caused by PM and DM infection.…”

Section: Discussionmentioning

confidence: 99%

Biological and molecular interplay between two viruses and powdery and downy mildews in two grapevine cultivars

et al. 2020

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Grapevine may be affected simultaneously by several pathogens whose complex interplay is largely unknown. We studied the effects of infection by two grapevine viruses on powdery mildew and downy mildew development and the molecular modifications induced in grapevines by their multiple interactions. Grapevine fanleaf virus (GFLV) and grapevine rupestris stem pitting-associated virus (GRSPaV) were transmitted by in vitro-grafting to Vitis vinifera cv Nebbiolo and Chardonnay virus-free plantlets regenerated by somatic embryogenesis. Grapevines were then artificially inoculated in the greenhouse with either Plasmopara viticola or Erysiphe necator spores. GFLV-infected plants showed a reduction in severity of the diseases caused by powdery and downy mildews in comparison to virus-free plants. GFLV induced the overexpression of stilbene synthase genes, pathogenesis-related proteins, and influenced the genes involved in carbohydrate metabolism in grapevine. These transcriptional changes suggest improved innate plant immunity, which makes the GFLV-infected grapevines less susceptible to other biotic attacks. This, however, cannot be extrapolated to GRSPaV as it was unable to promote protection against the fungal/oomycete pathogens. In these multiple interactions, the grapevine genotype seemed to have a crucial role: in ‘Nebbiolo’, the virus-induced molecular changes were different from those observed in ‘Chardonnay’, suggesting that different metabolic pathways may be involved in protection against fungal/oomycete pathogens. These results indicate that complex interactions do exist between grapevine and its different pathogens and represent the first study on a topic that still is largely unexplored.

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“…This hypothesis was tested by a ‘proof‐of‐concept’ approach using cleft‐grafting, part of the resistance screening, and testing root tissue which mites were unable to reach and feed on. Even though the virus was only present in 50% of the samples tested, this is not unusual as many viruses have uneven distribution in the plant (Harper et al ., ; Krebelj et al ., ; Wells‐Hansen & McManus, ). Pruning of areas with symptoms does not eliminate the virus and should be avoided so as to minimize the persistence of the virus after overwintering in the root system.…”

Section: Discussionmentioning

confidence: 99%

Transmission attributes and resistance to rose rosette virus

et al. 2017

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Rosette caused by rose rosette virus (RRV) is a devastating disease of rose in the United States. The virus was discovered in 2011 and Koch's postulates completed in 2015. Because of these recent discoveries, assumptions about the disease including movement, transmission and resistance are based on visual observations of material that may or may not have been infected by the virus. This study addresses several aspects of virus and disease dynamics. Twenty rose genotypes were screened for mite and/or virus resistance. Phyllocoptes fructiphilus, the only known vector of RRV, was able to establish, lay eggs and develop nymphs and adults in all rose genotypes. Cultivar ‘Stormy Weather’ showed resistance to the virus as assessed in both mite and cleft‐grafting transmission experiments. Mites showed a long acquisition/latent period but a rapid inoculation time for RRV. Knowledge of resistance as well as transmission attributes will assist in better management of vector and disease. The identified resistant genotype would be used in areas with high disease pressure to minimize spread, for identification of the mechanisms behind resistance or as a breeding parent to incorporate virus resistance to new cultivars. The short inoculation access period suggests that chemical control for this vector may be challenging to undertake.

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Spatio-temporal distribution of Grapevine fanleaf virus (GFLV) in grapevine

Cited by 16 publications

References 23 publications

Frequency of occurrence and genetic variability of Grapevine fanleaf virus satellite RNA

Frequency of occurrence and genetic variability of Grapevine fanleaf virus satellite RNA

Biological and molecular interplay between two viruses and powdery and downy mildews in two grapevine cultivars

Transmission attributes and resistance to rose rosette virus

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