Spatially Selecting a Single Cell for Lysis Using Light‐Induced Electric Fields

Witte, Christian; Kremer, Clemens; Chanasakulniyom, Mayuree; Reboud, Julien; Wilson, Rab; Cooper, Jonathan M.; Neale, Steven L.

doi:10.1002/smll.201400247

Cited by 16 publications

(14 citation statements)

References 38 publications

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“…The second reason is the potential to reduce cell damage caused by continuous exposure to bright illumination in OET. This effect has been described previously (28,(36)(37)(38), and we devised a unique small-cell-number viability assay to evaluate its potential effect here (SI Appendix, Fig. S2).…”

Section: Resultsmentioning

confidence: 99%

“…3J, viability is nearly unchanged after manipulation by microrobot, but it is reduced to 76% for ARPE-19 cells and 70% for MCF-7 cells when manipulated using OET alone. We attribute this effect to exposure to the large electric field at the edge of the light pattern, as well as light-induced heating, which can perforate the cell membrane (36)(37)(38). In fact, we find that killing cells by OET is remarkably easy--for example, Movie S8 illustrates nearly instantaneous lysis of MCF-7 cells by OET driven at 18-V p-p bias voltage; the same movie shows that microrobots driven by the same bias can be used to manipulate cells without harm.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

The optoelectronic microrobot: A versatile toolbox for micromanipulation

Zhang

Scott

Singh

et al. 2019

Proc. Natl. Acad. Sci. U.S.A.

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Microrobotics extends the reach of human-controlled machines to submillimeter dimensions. We introduce a microrobot that relies on optoelectronic tweezers (OET) that is straightforward to manufacture, can take nearly any desirable shape or form, and can be programmed to carry out sophisticated, multiaxis operations. One particularly useful program is a serial combination of “load,” “transport,” and “deliver,” which can be applied to manipulate a wide range of micrometer-dimension payloads. Importantly, microrobots programmed in this manner are much gentler on fragile mammalian cells than conventional OET techniques. The microrobotic system described here was demonstrated to be useful for single-cell isolation, clonal expansion, RNA sequencing, manipulation within enclosed systems, controlling cell–cell interactions, and isolating precious microtissues from heterogeneous mixtures. We propose that the optoelectronic microrobotic system, which can be implemented using a microscope and consumer-grade optical projector, will be useful for a wide range of applications in the life sciences and beyond.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

The optoelectronic microrobot: A versatile toolbox for micromanipulation

Zhang

Scott

Singh

et al. 2019

Proc. Natl. Acad. Sci. U.S.A.

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show abstract

“…Initially, low OET bias (0.2 kV/cm) was used to position individual cells in specified locations, followed by application of high electroporation bias (1.5 kV/cm) to selected cells resulting in the intracellular delivery of the PI dye (Figure e). In addition to electroporation, single cell lysis using OET has also been demonstrated (Kremer et al, ; Witte et al, ). Lastly, a device based on a novel concept, Self‐Locking Optoelectronic Tweezers (SLOT), has shown promising results in scaling up single‐cell manipulation across a significantly larger area (Y. Yang et al, ).…”

Section: Biological Applications Of Oetsmentioning

confidence: 99%

Nanoscale integration of single cell biologics discovery processes using optofluidic manipulation and monitoring

Jorgolli

Nevill²,

Winters

et al. 2019

Biotech & Bioengineering

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The new and rapid advancement in the complexity of biologics drug discovery has been driven by a deeper understanding of biological systems combined with innovative new therapeutic modalities, paving the way to breakthrough therapies for previously intractable diseases. These exciting times in biomedical innovation require the development of novel technologies to facilitate the sophisticated, multifaceted, high‐paced workflows necessary to support modern large molecule drug discovery. A high‐level aspiration is a true integration of “lab‐on‐a‐chip” methods that vastly miniaturize cellulmical experiments could transform the speed, cost, and success of multiple workstreams in biologics development. Several microscale bioprocess technologies have been established that incrementally address these needs, yet each is inflexibly designed for a very specific process thus limiting an integrated holistic application. A more fully integrated nanoscale approach that incorporates manipulation, culture, analytics, and traceable digital record keeping of thousands of single cells in a relevant nanoenvironment would be a transformative technology capable of keeping pace with today's rapid and complex drug discovery demands. The recent advent of optical manipulation of cells using light‐induced electrokinetics with micro‐ and nanoscale cell culture is poised to revolutionize both fundamental and applied biological research. In this review, we summarize the current state of the art for optical manipulation techniques and discuss emerging biological applications of this technology. In particular, we focus on promising prospects for drug discovery workflows, including antibody discovery, bioassay development, antibody engineering, and cell line development, which are enabled by the automation and industrialization of an integrated optoelectronic single‐cell manipulation and culture platform. Continued development of such platforms will be well positioned to overcome many of the challenges currently associated with fragmented, low‐throughput bioprocess workflows in biopharma and life science research.

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“…To date, OEK-based microfluidics chips have been widely used in the manipulation, assembly, and synthesis of micro/nanomaterials as well as molecular engineering. [29][30][31][32][33][34][35][36][37] OEK has also been found to be efficient for applications in immunology sciences, [38][39][40] nanosensors, 41,42 blood analysis and detection, 43,44 and biomedical and bioengineering. 45,46 Allowing direct optical addressing of micro/nano-objects by optically-projected images that act as virtual electrodes, the OEK-based microfluidics technique offers the following advantages over other competing techniques:…”

Section: Introductionmentioning

confidence: 99%

Optoelectrokinetics-based microfluidic platform for bioapplications: A review of recent advances

et al. 2019

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The introduction of optoelectrokinetics (OEK) into lab-on-a-chip systems has facilitated a new cutting-edge technique-the OEK-based micro/nanoscale manipulation, separation, and assembly processes-for the microfluidics community. This technique offers a variety of extraordinary advantages such as programmability, flexibility, high biocompatibility, low-cost mass production, ultralow optical power requirement, reconfigurability, rapidness, and ease of integration with other microfluidic units. This paper reviews the physical mechanisms that govern the manipulation of micro/nano-objects in microfluidic environments as well as applications related to OEK-based micro/nanoscale manipulation-applications that span from single-cell manipulation to single-molecular behavior determination. This paper wraps up with a discussion of the current challenges and future prospects for the OEK-based microfluidics technique. The conclusion is that this technique will allow more opportunities for biomedical and bioengineering researchers to improve lab-on-a-chip technologies and will have farreaching implications for biorelated researches and applications in the future.

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Spatially Selecting a Single Cell for Lysis Using Light‐Induced Electric Fields

Cited by 16 publications

References 38 publications

The optoelectronic microrobot: A versatile toolbox for micromanipulation

The optoelectronic microrobot: A versatile toolbox for micromanipulation

Nanoscale integration of single cell biologics discovery processes using optofluidic manipulation and monitoring

Optoelectrokinetics-based microfluidic platform for bioapplications: A review of recent advances

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