Spatially resolved detection of antibody—antigen reaction on solid/liquid interface using total internal reflection excited antigen fluorescence and charge-coupled device detection

Hlady, Vladimir; Lin, Jinn-Nan; Andrade, Joseph D.

doi:10.1016/0956-5663(90)85002-t

Cited by 22 publications

(5 citation statements)

References 8 publications

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“…The 30-min exposure time was assumed sufficient for allowing a reasonable amount of bacteria in the microfluidic chamber to interact with the antibody sites of the biochip surface [66]. This step could be refined further, 5.…”

Section: Photonic Detection Of E Coli In Pbsmentioning

confidence: 99%

GaAs/AlGaAs heterostructure based photonic biosensor for rapid detection of Escherichia coli in phosphate buffered saline solution

Nazemi

Aithal

Hassen

et al. 2015

Sensors and Actuators B: Chemical

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Section: Photonic Detection Of E Coli In Pbsmentioning

confidence: 99%

GaAs/AlGaAs heterostructure based photonic biosensor for rapid detection of Escherichia coli in phosphate buffered saline solution

Nazemi

Aithal

Hassen

et al. 2015

Sensors and Actuators B: Chemical

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“…Maybe even more interesting is the measurement of the adsorption kinetics of polymers, peptides, proteins, and biomembranes, and the adhesion dynamics of living cells. Several techniques have been applied successfully to some of these deposition types (Lundstrom, 1985;Horbett and Brash, 1995;Brash and Wojciechowski, 1996;Hlady and Buijs, 1996;Norde, 1996), e.g., ellipsometry (Arwin, 1998;Elwing, 1998;Tengvall etal., 1998), surface plasmon resonance (Kosslinger et al, 1995), and total internal reflection spectroscopy (Axelrod et al, 1984;Hlady et al, 1990).…”

Section: (4) In Vitro Evaluation Of Biomaterials Surface Preparation Amentioning

confidence: 99%

Implant Surfaces and Interface Processes

1999

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The past decades and current R&D of biomaterials and medical implants show some general trends. One major trend is an increased degree of functionalization of the material surface, better to meet the demands of the biological host system. While the biomaterials of the past and those in current use are essentially bulk materials (metals, ceramics, polymers) or special compounds (bioglasses), possibly with some additional coating (e.g., hydroxyapatite), the current R&D on surface modifications points toward much more complex and multifunctional surfaces for the future. Such surface modifications can be divided into three classes, one aiming toward an optimized three-dimensional physical microarchitecture of the surface (pore size distributions, "roughness", etc.), the second one focusing on the (bio) chemical properties of surface coatings and impregnations (ion release, multi-layer coatings, coatings with biomolecules, controlled drug release, etc.), and the third one dealing with the viscoelastic properties (or more generally the micromechanical properties) of material surfaces. These properties are expected to affect the interfacial processes cooperatively, i.e., there are likely synergistic effects between and among them: The surface is "recognized" by the biological system through the combined chemical and topographic pattern of the surface, and the viscoelastic properties. In this presentation, the development indicated above is discussed briefly, and current R&D in this area is illustrated with a number of examples from our own research. The latter include micro- and nanofabrication of surface patterns and topographies by the use of laser machining, photolithographic techniques, and electron beam and colloidal lithographies to produce controlled structures on implant surfaces in the size range 10 nm to 100 microns. Examples of biochemical modifications include mono- or lipid membranes and protein coatings on different surfaces. A new method to evaluate, e.g., biomaterial-protein and biomaterial-cell interactions--the Quartz Crystal Microbalance--is described briefly.

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“…Imaging techniques using dove prisms with single total internal reflection configurations have been developed. , An imaging system for microarrays on single-mode planar waveguides has been described . The used waveguides have very high sensitivity but are not compatible with standard readout systems and are high in cost.…”

mentioning

confidence: 99%

Real-Time Detection of Nucleic Acid Interactions by Total Internal Reflection Fluorescence

Reimann

Brandenburg

et al. 2003

Anal. Chem.

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This paper describes the development of an optical readout system for the real-time analysis of fluorescent-labeled DNA microarrays is described. The system is targeted toward research applications in genomics, agriculture, and life sciences, where the end-point detection of state-of-the-art readout systems does not provide sufficient information on the hybridization process. The hybridization progress of molecules from the liquid phase in a flow cell to immobilized oligonucleotides on a transducer surface can be observed. The excitation of fluorochromes is realized by a semiconductor laser, and the fluorescence emission is collected by a cooled CCD camera. Quantitative data can be extracted from the images for analysis of the microarray. For the signal transduction, the principle of total internal reflection is used. With a multiple internal reflection arrangement, the sensor chip was adapted to the standard microscope slide format and a homogeneous evanescent illumination of the active area of the sensor surface was achieved. An application measurement was carried out with this readout system. The hybridization of Cy5-labeled 30-mer single-stranded oligonucleotides to fully complementary immobilized strands was observed in real time. A kinetic analysis was demonstrated with the recorded data. Melting curves of a 140-mer PCR product from a hemochromatosis patient sample hybridized to immobilized wild-type mutant 15- and 17-mer oligonucleotides were recorded and single-point mutations could be detected.

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Spatially resolved detection of antibody—antigen reaction on solid/liquid interface using total internal reflection excited antigen fluorescence and charge-coupled device detection

Cited by 22 publications

References 8 publications

GaAs/AlGaAs heterostructure based photonic biosensor for rapid detection of Escherichia coli in phosphate buffered saline solution

GaAs/AlGaAs heterostructure based photonic biosensor for rapid detection of Escherichia coli in phosphate buffered saline solution

Implant Surfaces and Interface Processes

Real-Time Detection of Nucleic Acid Interactions by Total Internal Reflection Fluorescence

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