Methane is emitted in horizontal subsurface flow constructed wetlands (SSF CW) during wastewater treatment. The objective of this work was to determine the influence of primary treatment and organic loading rate on methane emissions from constructed wetlands. To this aim methane emissions from a SSF CW pilot plant were measured using the closed chamber method. The effect of primary treatment was addressed by comparing emissions from wetlands operated either with an anaerobic (HUSB reactor) and with or a conventional settler as primary treatments. Alternatively, the effect of organic loading was addressed by comparing emissions from wetlands operated under high organic loading (52 g COD.m -2 .day -1 ) and low organic loading (17 g COD.m -2 .day -1 ). Results suggest that SSF CW redox status at the middle part of the treatment bed (15 cm depth) is of high reduced nature, regardless the type of primary treatment or organic loading applied. However, redox conditions on the upper part of the wetlands (5 cm depth) are especially affected by the type of primary treatment implemented. Accordingly, significantly lower redox conditions at 5 cm depth in wetlands receiving HUSB effluents are recorded which, in turn, resulted in significant lower organic matter removal efficiencies. Moreover, methane emission rates are affected by the type of primary treatment and, to a lesser extent, by the organic loading applied. Accordingly, a wetland fed with the effluent of a HUSB line were up to 14 times higher than those of the wetland fed with primary settled wastewater. Moreover, systems subjected to three times higher organic loading than that recommended lead to higher metheane emission rates, although high data variability resulted in no statistically significant differences.