Spatial–Temporal Co-Circulation of Dengue Virus 1, 2, 3, and 4 Associated with Coinfection Cases in a Hyperendemic Area of Brazil: A 4-Week Survey

Andrade, Elisa Helena Paz; Figueiredo, Leandra Barcelos; Vilela, Ana Paula Pessoa; Rosa, Júlio César Câmara; Oliveira, Jaquelline Germano de; Zibaoui, Hassan Melo; Araújo, Valdelaine Etelvina Miranda de; Miranda, Daniela P J; Ferreira, Paulo César Peregrino; Abrahão, Jônatas Santos; Kroon, Erna Geessien

doi:10.4269/ajtmh.15-0892

Cited by 34 publications

(25 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Also, we report for the first time genomic-based evidence of dengue virus co-infection with DENV-1 and DENV-3 serotypes during a single phase of outbreak in Tanzania. Dengue concurrent infections have been commonly reported in endemic countries including Brazil [29] and India [30]. Dengue concurrent infections have serious clinical implication for patient management, as they can present with severe clinical manifestations such as haemorrhagic fever syndrome [31,32].…”

Section: Discussionmentioning

confidence: 99%

Co-circulation of Dengue Virus Serotypes 1 and 3 during the 2019 epidemic in Dar es Salaam, Tanzania

Mwanyika

Mboera

Rugarabamu

et al. 2019

Preprint

View full text Add to dashboard Cite

22 23 *gaspary.mwanyika@sacids.org 24 25 26 27 PLOS Neglected Tropical Diseases 2 28 Abstract 29 Background 30 Dengue is an important mosquito-borne viral disease in tropical and sub-tropical 31 countries. In this study molecular characterization was carried out to determine 32 dengue viruses circulating among patients at health facilities during 2019 epidemic in 33 Dar es Salaam, Tanzania. 34 Methods 35 The study involved outpatients seeking care for febrile illness at four health facilities 36 in Kinondoni and Ilala Districts of Dar es Salaam City in Tanzania. A total of 45 sera 37 from the outpatients were confirmed dengue-positive for dengue virus (DENV) non-38 structural protein 1 (NS1) antigen and/or NS1-IgG/IgM antibodies using on-site rapid 39 test. The presence of the virus was detected by reverse-transcription polymerase 40 chain reaction (RT-PCR) method. Of the 45 sera, 20 samples were selected 41 randomly for identification of specific dengue virus serotypes using RT-PCR followed 42 by evaluation of resulting amplicons on agarose gel electrophoresis. 43 Findings and significance 44 Both Dengue virus serotypes 1 (DENV-1) and 3 (DENV-3) were detected in the 45 samples tested with the former being dominant. We present the first evidence of 46 dengue virus co-infection of DENV-1 and DENV-3 serotypes in Tanzania. The 47 emergence of DENV-1 indicates the possibility of importation of the virus to Tanzania 48 from endemic countries. Due to DENV serotype co-circulation, there is an increased 49 risk of severe dengue in future epidemics. Our findings advocate the importance of 50 genomic-based surveillance to provide rapid evidence of dengue virus 51 emergence/re-emergence and spread. 52 Author Summary 54 Dengue viruses are the most important mosquito-borne pathogens that pose a 55 serious global health threat. Tanzania has reported several dengue virus epidemics 56 since 2010 with the majority of the epidemics occurring in Dar-es-Salaam city. Until 57 August 2019, a total of 6,859 dengue cases have been confirmed in the country. We 58 performed molecular characterization of dengue viruses (DENV) circulating during 59 the 2019 epidemic phase. It was found that DENV-1 serotype was dominant during 60 the epidemic and two samples of the tested sera were co-infected by DENV-1 and 61

show abstract

Section: Discussionmentioning

confidence: 99%

Co-circulation of Dengue Virus Serotypes 1 and 3 during the 2019 epidemic in Dar es Salaam, Tanzania

Mwanyika

Mboera

Rugarabamu

et al. 2019

Preprint

View full text Add to dashboard Cite

show abstract

“…One lineage was estimated to be introduced into Divinópolis around 2007, and the second was estimated to be introduced into the city around 2011 and those lineages co‐circulated at least during 2013 epidemics. Data from Andrade et al [] suggests the co‐circulation of distinct lineages in other part of Minas Gerais state. In addition, analysis based on envelope sequences obtained from isolates of Rio de Janeiro (in 2010) and Pernambuco (in 2010) also indicated the co‐circulation of lineages BR3 and BR5 in these years in Southeast, Northeast regions, respectively.…”

Section: Discussionmentioning

confidence: 99%

Molecular surveillance of dengue in Minas Gerais provides insights on dengue virus 1 and 4 circulation in Brazil

Dutra

Drumond

Rezende

et al. 2016

Journal of Medical Virology

View full text Add to dashboard Cite

Dengue, caused by any of the four types of Dengue virus (DENV) is the most important arbovirus in the world. In this study we performed a molecular surveillance of dengue during the greatest dengue outbreak that took place in Divinópolis, Minas Gerais state, Southeast Brazil, in 2013. Samples from 100 patients with clinical symptoms of dengue were studied and 26 were positive. The capsid/premembrane (CprM) and envelope gene sequences of some samples were amplified and sequenced. Molecular analyses demonstrated that two DENV-1 lineages, belonging to genotype V were introduced and co-circulated in Divinópolis. When compared to each other, those lineages presented high genetic diversity and showed unique amino acids substitutions in the envelope protein, including in domains I, II, and III. DENV-4 strains from Divinópolis clustered within genotype IIb and the most recent common ancestor was probably introduced into the city three years before the 2013 epidemic. Here we demonstrated for the first time the circulation of DENV-4 and the co-circulation of two DENV-1 lineages in Midwest region of Minas Gerais, Brazil. Moreover our analysis indicated the introduction of five DENV-1 lineages, genotype V into Brazil, in different times. J. Med. Virol. 89:966-973, 2017. © 2016 Wiley Periodicals, Inc.

show abstract

“…17 Concurrent circulation of all four serotypes has also been reported in South America and Asia. [29][30][31][32] We provide evidence for the co-circulation of three serotypes. Dengue virus-4 was not detected, although nationwide surveillance data collected in 2000-2011 revealed that DENV-4 is the least common serotype in Mexico.…”

Section: Discussionmentioning

confidence: 74%

Surveillance for Flaviviruses Near the Mexico–U.S. Border: Co-circulation of Dengue Virus Serotypes 1, 2, and 3 and West Nile Virus in Tamaulipas, Northern Mexico, 2014–2016

Laredo-Tiscareño

Garza-Hernández

Salazar

et al. 2018

The American Journal of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

A clinical, serological, and molecular investigation was performed to determine the presence of dengue virus (DENV) and other flaviviruses among residents of the city of Reynosa, Tamaulipas, on the Mexico-U.S. border in 2014-2016. The sample population consisted of 2,355 patients with suspected dengue, in addition to 346 asymptomatic individuals recruited during a household-based epidemiological investigation designed to identify flavivirus seroconversions. Sera were collected from patients with suspected dengue in the acute phase of illness and from asymptomatic individuals at enrollment and every 5-7 months for 19 months. Sera from suspected dengue patients were tested for DENV antigen by enzyme-linked immunosorbent assay (ELISA), and select antigen-positive sera were further tested using a serotype-specific, quantitative reverse transcription-polymerase chain reaction. Sera from the household cohort were tested for flavivirus-reactive antibodies by immunoglobulin (Ig) M and IgG ELISAs using DENV antigen. A total of 418 (17.7%) patients with suspected dengue had laboratory-confirmed DENV infections, including 82 patients who were positive for DENV RNA. The most frequently detected serotype was DENV-1 (61 patients), followed by DENV-2 (16 patients) and DENV-3 (five patients). A total of 217 (62.7%) asymptomatic individuals had flavivirus-reactive antibodies at enrollment, and nine flavivirus-naïve individuals seroconverted. Sera from a subset of dengue patients and household participants, including all those who seroconverted, were further tested by plaque reduction neutralization test, resulting in the detection of antibodies to DENV-1, DENV-2, and West Nile virus. In summary, we provide evidence for the co-circulation of multiple flaviviruses in Reynosa, Tamaulipas, on the Mexico-U.S. border.

show abstract

Spatial–Temporal Co-Circulation of Dengue Virus 1, 2, 3, and 4 Associated with Coinfection Cases in a Hyperendemic Area of Brazil: A 4-Week Survey

Cited by 34 publications

References 14 publications

Co-circulation of Dengue Virus Serotypes 1 and 3 during the 2019 epidemic in Dar es Salaam, Tanzania

Co-circulation of Dengue Virus Serotypes 1 and 3 during the 2019 epidemic in Dar es Salaam, Tanzania

Molecular surveillance of dengue in Minas Gerais provides insights on dengue virus 1 and 4 circulation in Brazil

Surveillance for Flaviviruses Near the Mexico–U.S. Border: Co-circulation of Dengue Virus Serotypes 1, 2, and 3 and West Nile Virus in Tamaulipas, Northern Mexico, 2014–2016

Contact Info

Product

Resources

About