Spatial distribution of parasitism on Phyllocnistis citrella Stainton, 1856 (Lepidoptera: Gracillariidae) in citrus orchards

Abstract

Many species of microhymenopterous parasitoids have been registered on Phyllocnistis citrella, the citrus leafminer. The present study aimed to identify the spatial distribution pattern of the native and introduced parasitoids of P. citrella in two citrus orchards in Montenegro, RS. The new shoots from 24 randomly selected trees in each orchard were inspected at the bottom (0-1.5 m) and top (1.5-2.5 m) stratum and had their position relative to the quadrants (North, South, East and West) registered at every 15 days from July/2002 to June/2003. The leaves with pupae were collected and kept isolated until the emergence of parasitoids or of the leaf miner; so, the sampling was biased towards parasitoids that emerge in the host pupal phase. The horizontal spatial distribution was evaluated testing the fitness of data to the Poisson and negative binomial distributions. In Montenegrina, there was no significant difference in the number of parasitoids and in the mean number of pupae found in the top and bottom strata (χ 2 = 0.66; df = 1; P > 0.05) (χ 2 = 0.27; df =1; P > 0.05), respectively. In relation to the quadrants, the highest average numbers of the leafminer pupae and of parasitoids were registered at the East quadrant (χ 2 = 11.81; df = 3; P < 0.05), (χ 2 = 10.36; df = 3; P < 0.05). In the Murcott orchard, a higher number of parasitoids was found at the top stratum (63.5%) (χ 2 = 7.24; df =1 P < 0.05), the same occurring with the average number of P. citrella pupae (62.9%) (χ 2 = 6.66; df = 1; P < 0.05). The highest number of parasitoids and of miners was registered at the North quadrant (χ 2 = 19. 29; df = 3; P < 0.05), (χ 2 = 4.39; df = 3; P < 0.05). In both orchards, there was no difference between the numbers of shoots either relative to the strata as well as to the quadrants. As the number of shoots did not varied much relative to the quadrants, it is possible that the higher number of miners and parasitoids in the East and West quadrants would be influenced by the higher solar exposure of these quadrants. The data of the horizontal spatial distribution of the parasitism fit to the negative binomial distribution in all sampling occasions, indicating an aggregated pattern.Keywords: citrus leafminer, biological control, parasitoids, Hymenoptera. Distribuição espacial do parasitismo Phyllocnistis citrella Stainton, 1856(Lepidoptera: Gracillaridae) em pomares de citros ResumoVários microimenópteros parasitóides têm sido registrados em populações de Phyllocnistis citrella (minador-doscitros). Este estudo objetivou identificar o padrão de distribuição espacial de parasitóides nativos de P. citrella e do introduzido Ageniaspis citricola em dois pomares de citros em Montenegro, RS. Em amostragens quinzenais de julho de 2002 a junho de 2003, todos os brotos de 24 plantas sorteadas aleatoriamente em cada pomar foram inspecionados no estrato inferior (0-1,5 m) e superior (1,5-2,5 m) e registrada a localização destes em relação aos quadrantes (Norte, Sul, Leste e Oeste). As folhas com pupas foram coletadas e i...

Avoidance of parasitoid attack is associated with the spatial use within a leaf by a lepidopteran leafminer

“…By carefully monitoring every miner individual until its fate was determined, we demonstrated that the patterns of parasitoid attack could affect the fitness of leafminers at a local (within-leaf) scale and the attack patterns varied depending on mine density and ratios of upper-to lower-surface mines. Studies have demonstrated that mine distribution at a within-tree scale can cause specific patterns of parasitoid attack (Barrett, 1994;Jahnke et al, 2008). Our findings suggest that the within-leaf spatial distribution of mines also can be used to evaluate the effects of oviposition behavior of parasitoids on leafminer fitness and the susceptibility of leafminers to parasitism.…”

“…Studies have demonstrated that mine distribution at a within-tree scale can cause specific patterns of parasitoid attack (Barrett, 1994;Jahnke et al, 2008). By carefully monitoring every miner individual until its fate was determined, we demonstrated that the patterns of parasitoid attack could affect the fitness of leafminers at a local (within-leaf) scale and the attack patterns varied depending on mine density and ratios of upper-to lower-surface mines.…”

Spatial Patterns and Associations ofAnastrepha fraterculus(Diptera: Tephritidae) and Its ParasitoidDoryctobracon areolatus(Hymenoptera: Braconidae) in Organic Orchards ofPsidium guajavaandAcca sellowiana

“…It is well known that the heterogeneity of the habitat is the most obvious cause of aggregation (Poole 1974). Abiotic factors such as light, temperature, wind, humidity and other ecological factors such as competition for food, predation and sexual stimuli, may influence the patterns of aggregation of individuals in a population (Farias et al 2001;Jahnke et al 2008). In our case, a random spatial arrangement of the insect counts in one tree related to other trees may be related to a combination of factors such as the nutritional status of the plants, the availability of fruits and their nutritional quality, as well as characteristics of the surroundings of these orchards.…”

First report of leafminer Lyriomyza spp. in Daucus carota in Brazil

“…Diptera, Lepidoptera, and Coleoptera are the taxonomic groups of leaf miners more common of the world (Foba et al, 2015;Jahnke et al, 2008;Queiroz, 2002;Marchiori et al, 2004). The dipterous agromyzid leaf miner, Lyriomiza spp., are a more important polyphagous pest of the vegetables species of plants (Foba et al, 2015) in the Americas.…”