Spatial Abundance, Diversity, and Activity of Ammonia-Oxidizing Bacteria in Coastal Sediments of the Liaohe Estuary

Chang, Yen‐Chiang; Fan, Jing-Li; Su, Jie; Ming, Hongxia; Zhao, Wen; Shi, Yan; Ji, Fengyun; Guo, Limei; Zan, Shuaijun; Li, Bochao; Guo, Hao; Guan, De‐Long

doi:10.1007/s00284-017-1226-x

Cited by 9 publications

(4 citation statements)

References 36 publications

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“…A similar phylum composition structure was found in previous wetland soil investigations in the Jiulong River estuary [21] and intertidal sediments in the Yellow Sea [22]. As the dominant phylum in soil samples, Proteobacteria was involved in nitrification, denitrification, and sulphuration [23,24]. Playing the leading role in a large number of processes related to the nitrogen cycle and sulfur…”

Section: Composition and Structure Of The Bacterial Communitysupporting

confidence: 78%

Diversity and Structure of Vegetation Rhizosphere Bacterial Community in Various Habitats of Liaohekou Coastal Wetlands

Liu

Zhang

et al. 2022

Sustainability

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Coastal wetlands are a type of unique ecosystem, in which rhizosphere microorganisms of vegetation play a significant role in the overall ecology. Rhizosphere soil samples from the vegetation of Liaohekou Coastal Wetlands, Northeast China (40°54′44″ N, 121°47′51″ E), were collected in seven habitats (Suaeda and Phragmites community in different coverage, aquaculture ponds and farmland biotopes) to evaluate the diversity and structure of bacterial community using high throughput sequencing. Soil physicochemical characteristics and bacterial communities were found to be affected by vegetation coverage by ANOVA tests. As and Ni were the main heavy metal variables affecting the bacterial communities as demonstrated by RDA tests, while NO3−-N were important variables in nutrient factors. Proteobacteria was the predominant phylum in all soils. Gillisia and Woeseia were the two most dominant genera peculiarly in Suaeda and Phragmites community. Meanwhile SparCC showed that Woeseia play a dominant role in wetland rhizosphere bacterial communities. The Chemoheterotrophic function was dominant in all communities with FAPROTAX results, while in wetland the cycle of Sulfur and Nitrogen were significantly affected by vegetation type and coverage. In conclusion, this study revealed the structural composition and diversity of rhizosphere bacterial communities under different vegetation types and coverage. This research could help deepen our understanding of the microbial ecology on the wetlands and provide information on bacterial communities in various habitats.

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Section: Composition and Structure Of The Bacterial Communitysupporting

confidence: 78%

Diversity and Structure of Vegetation Rhizosphere Bacterial Community in Various Habitats of Liaohekou Coastal Wetlands

Liu

Zhang

et al. 2022

Sustainability

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“…Öre River), whereas Nitrosospira ‐like OTUs were confined to the rivers, indicating a preference for freshwater. Both genera have, however, previously been found in Baltic Sea waters and sediments (Kim et al ., ; Vetterli et al ., ), and in sediments (Chang et al ., ) and waters (Bano and Hollibaugh, ; O'Mullan and Ward, ; Kim et al ., ) from multiple localities. It is not understood what governs the distribution of the two genera, possibly as a consequence of difficulties associated with correlating single environmental factors to complex combinations of variables shaping AOB diversity in dynamic environments (Francis et al ., ).…”

Section: Discussionmentioning

confidence: 99%

Extensive nitrification and active ammonia oxidizers in two contrasting coastal systems of the Baltic Sea

Happel

Bartl

Voß

et al. 2018

Environmental Microbiology

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Nitrification is important in nitrogen (N) cycling of aquatic environments, but knowledge about its regulation and importance is sparse. Here we examined nitrification and ammonia oxidizers in the Baltic Sea. We investigated two sites with different catchment characteristics (agricultural and forest), the Bay of Gdánsk (south) and the Öre Estuary (north), and measured pelagic nitrification rates and abundance, composition and expression of ammonia monooxygenase (amoA) genes. Highest nitrification rates were found in the nutrient rich Bay of Gdańsk. Interestingly, abundances of ammonia-oxidizing archaea (AOA) and bacteria (AOB) were orders of magnitude lower than reported from other sites. Although AOA were most abundant at both sites, the highest expression levels were from AOB. Interestingly, few AOA and AOB taxa dominated amoA gene expression, with a Nitrosomarinus related phylotype showing widespread expression. AOA and AOB communities differed between sites and depths, respectively, with the composition in rivers being distinct. A storm event, causing an even depth distribution of nitrification and particles in the Bay of Gdańsk, indicated that the presence of particles stimulate nitrification. The study highlights coastal regions as dynamic sites of extensive pelagic nitrification, which may affect local food web dynamics and loss of N mediated by denitrification.

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“…The primer sequences for the soil nitrification microbial amoA gene were A26F (5′-GACTACATMTTCTAYACWGAYTGGGC-3′) and A416R (5′-GGKGTCATRTATGG WGGYAAYGTTGG-3′) ( Francis et al, 2005 ). The AOA amoA and AOB amoA genes were amplified with primer sequences amoA -1F (5′-GGGGTTTCTACTGGTGGT-3′) and amoA -1R (5′-CCCCTCKGSAAAGCCTTCTTC-3′), respectively ( Chang et al, 2017 ). The reaction system was as follows: 10 μM upstream primer and downstream primer 1 μL, 1 μL SYBR ® PremixExTaq™II (TliRNaseHPlus), ROXplusL, 25 μL 2 × TaqMasterMix, and 22 μL water, making a total of 50 μL.…”

Section: Methodsmentioning

confidence: 99%

“…Javed et al (2021) confirmed that the abundance of Actinobacteria bacteria can promote the formation of humus and decomposition of organic matter in soil, and also have a certain effect on the soil N cycle. As Actinobacteria tends to grow in soil with low SOM content, film mulching reduces the organic carbon content of rhizosphere soil, which is conducive to promoting the growth and propagation of Actinobacteria (Chen et al, 2017). According to Blast analysis, Nitrososphaerota is an important strain of AOA, and Nitrososphaera belongs to Nitrososphaerota.…”

Section: Effects Of Irrigation Methods On the Composition Of Ammonia-...mentioning

confidence: 99%

The different responses of AOA and AOB communities to irrigation systems in the semi-arid region of Northeast China

Qiang,

Wang,

et al. 2024

Front. Microbiol.

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Ammonia oxidation is the rate-limiting step in nitrification and the key step in the nitrogen (N) cycle. Most soil nutrients and biological indicators are extremely sensitive to irrigation systems, from the perspective of improving soil fertility and soil ecological environment, the evaluation of different irrigation systems and suitability of selection, promote crop production and soil quality, study the influence of the soil microenvironment contribute to accurate evaluation of irrigation farmland soil health. Based on the amoA gene, the abundance and community diversity of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) and their responses to soil physicochemical factors and enzyme activities were studied in semi-arid areas of Northeast China. The study consisted of three irrigation systems: flood irrigation (FP), shallow buried drip irrigation (DI), and mulched drip irrigation (MF). The results showed that DI and MF significantly increased the contents of alkaline hydrolyzed nitrogen (AN), nitrate nitrogen (NO3–-N), soil moisture, and the activities of ammonia monooxygenase (AMO) and hydroxylamine oxidase (HAO). Compared with FP, DI significantly increased the abundance of soil AOA and AOB, while MF significantly increased the abundance of soil AOB. Irrigation systems significantly affected the community composition of ammonia-oxidizing microorganisms (AOM). Also, AN and soil moisture had the greatest influence on the community composition of AOA and AOB, respectively. The AOB community had better stability and stress resistance. Moreover, the symbiotic network of AOB in the three irrigation systems was more complex than that of AOA. Compared with FP, the AOA community under treatment DI had higher complexity and stability, maintaining the versatility and sustainability of the ecosystem, while the AOB community under treatment MF had higher transfer efficiency in terms of matter and energy. In conclusion, DI and MF were more conducive to the propagation of soil AOM in the semi-arid area of Northeast China, which can provide a scientific basis for rational irrigation and N regulation from the perspective of microbiology.

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Spatial Abundance, Diversity, and Activity of Ammonia-Oxidizing Bacteria in Coastal Sediments of the Liaohe Estuary

Cited by 9 publications

References 36 publications

Diversity and Structure of Vegetation Rhizosphere Bacterial Community in Various Habitats of Liaohekou Coastal Wetlands

Diversity and Structure of Vegetation Rhizosphere Bacterial Community in Various Habitats of Liaohekou Coastal Wetlands

Extensive nitrification and active ammonia oxidizers in two contrasting coastal systems of the Baltic Sea

The different responses of AOA and AOB communities to irrigation systems in the semi-arid region of Northeast China

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