Spacer oligotyping of Mycobacterium bovis isolates compared to typing by restriction fragment length polymorphism using PGRS, DR and IS6110 probes

Roring, Solvig; Brittain, David; Bunschoten, Annelies; Hughes, M S; Skuce, Robin; Embden, J. D. A. Van; Neill, S.D.

doi:10.1016/s0378-1135(98)00178-3

Cited by 42 publications

(39 citation statements)

References 12 publications

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“…In agreement with previous comparative studies, LM-PCR was found to be more discriminatory than spoligotyping (9, 16) and slightly less discriminatory than IS6110 RFLP (43,58). Also consistent with previous reports, both VNTR typing methods and first-and second-generation spoligotyping were more useful than IS6110 RFLP for discriminating strains containing few IS6110 copies (7,24,37,43,55).…”

Section: Discussionsupporting

confidence: 91%

Discriminatory Power and Reproducibility of Novel DNA Typing Methods forMycobacterium tuberculosisComplex Strains

et al. 2005

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In recent years various novel DNA typing methods have been developed which are faster and easier to perform than the current internationally standardized IS6110 restriction fragment length polymorphism typing method. However, there has been no overview of the utility of these novel typing methods, and it is largely unknown how they compare to previously published methods. In this study, the discriminative power and reproducibility of nine recently described PCR-based typing methods for Mycobacterium tuberculosis were investigated using the strain collection of the interlaboratory study of Kremer et al. (J. Clin. Microbiol. 37:2607-2618, 1999). This strain collection contains 90 M. tuberculosis complex and 10 non-M. tuberculosis complex mycobacterial strains, as well as 31 duplicated DNA samples to assess reproducibility. The highest reproducibility was found with variable numbers of tandem repeat typing using mycobacterial interspersed repetitive units (MIRU VNTR) and fast ligation-mediated PCR (FLiP), followed by second-generation spoligotyping, ligation-mediated PCR (LM-PCR), VNTR typing using five repeat loci identified at the Queens University of Belfast (QUB VNTR), and the Amadio speciation PCR. Poor reproducibility was associated with fluorescent amplified fragment length polymorphism typing, which was performed in three different laboratories. The methods were ordered from highest discrimination to lowest by the Hunter-Gaston discriminative index as follows: QUB VNTR typing, MIRU VNTR typing, FLiP, LM-PCR, and spoligotyping. We conclude that both VNTR typing methods and FLiP typing are rapid, highly reliable, and discriminative epidemiological typing methods for M. tuberculosis and that VNTR typing is the epidemiological typing method of choice for the near future.

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Section: Discussionsupporting

confidence: 91%

Discriminatory Power and Reproducibility of Novel DNA Typing Methods forMycobacterium tuberculosisComplex Strains

et al. 2005

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“…In this case, not only did SB0273 show the highest macroscopical median score, but this score even increased without the older animals (28.5). SB520 (17) and SB0153 (17) followed those values.…”

Section: Resultsmentioning

confidence: 64%

“…SB0520 has been described only in Argentina. 11 SB0145 has been described as the most extensively distributed genotype, found in Argentina, 9,10,12,13 Australia, 14 Brazil, 15 France, 5 Northern Ireland, 16,17 and other countries (as seen at http://www.mbovis.org).…”

Section: Resultsmentioning

confidence: 99%

Association between spoligotype-VNTR types and virulence ofMycobacterium bovisin cattle

Garbaccio¹,

Macias

Shimizu

et al. 2014

Virulence

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“…Spoligotyping is considered a very useful technique, at least at the level of a first screening (26,35), and the results are produced in an intrin-sically qualitative form, as the response for each spacer is either present or absent (15 The geographical distribution of the French isolates for which a spoligotype was obtained is represented in Fig. 1.…”

mentioning

confidence: 99%

Spoligotype Diversity of Mycobacterium bovis Strains Isolated in France from 1979 to 2000

Haddad¹,

Ostyn²,

Karoui³

et al. 2001

J Clin Microbiol

123

107

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The molecular fingerprints of 1,349 isolates of Mycobacterium bovis received between 1979 and August 2000 at Agence Française de Sécurité Sanitaire des Aliments (Afssa) have been obtained by spoligotyping. The majority of the isolates (1,266) were obtained from cattle living in France. An apparently high level of heterogeneity was observed between isolates. One hundred sixty-one spoligotypes were observed in total, of which 153 were from French isolates. The two predominant spoligotypes, designated BCG-like and GB54, accounted for 26 and 12% of the isolates, respectively. In addition, 84% of the spoligotypes were found fewer than 10 times. Analysis of the results by clustering and parsimony-based algorithms revealed that the majority of the spoligotypes were closely related. The predominant spoligotype was identical to that of the vaccine strain Mycobacterium bovis BCG, which was isolated in France at the end of the 19th century. Some spoligotypes were closely associated with restricted geographical areas. Interestingly, some spoligotypes, which were frequently observed in France, were also observed in neighboring countries. Conversely, few spoligotypes were common to France and England, and those that were shared were observed at very different frequencies. This last point illustrates the potential role for an international data bank, which could help trace the spread of M. bovis across national borders.Bovine tuberculosis (TB) was endemic in France until the 1960s, with herd prevalence rates of 25% in 1955 (9). From this time onwards, a national program for TB control based on tuberculin skin testing with control of animal movements and total slaughter of infected herds was implemented. This control strategy resulted in a dramatic decrease in bovine tuberculosis leading to a herd prevalence rate of 0.09% in 1998 (2), suggesting that cattle are the most important reservoir, or even the sole reservoir, for Mycobacterium bovis in France. Due to the success of this control strategy, France was declared "officially free of bovine TB" by the European Commission (3).The very low level of TB in cattle has resulted in the introduction of new control strategies. Consequently, there has been a progressive reduction in the use of skin testing, with an increasing emphasis on systematic sampling of suspect lesions identified at slaughterhouses for M. bovis isolate identification and molecular typing. New laboratory tools were therefore required in order to improve the traceability of the infections and identification of the origin of the outbreak (i.e

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Spacer oligotyping of Mycobacterium bovis isolates compared to typing by restriction fragment length polymorphism using PGRS, DR and IS6110 probes

Cited by 42 publications

References 12 publications

Discriminatory Power and Reproducibility of Novel DNA Typing Methods forMycobacterium tuberculosisComplex Strains

Discriminatory Power and Reproducibility of Novel DNA Typing Methods forMycobacterium tuberculosisComplex Strains

Association between spoligotype-VNTR types and virulence ofMycobacterium bovisin cattle

Spoligotype Diversity of Mycobacterium bovis Strains Isolated in France from 1979 to 2000

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