Association between spoligotype-VNTR types and virulence of<i>Mycobacterium bovis</i>in cattle

Garbaccio, Sergio; Macias, Analía; Shimizu, Ernesto; Paolicchi, Fernando Alberto; Pezzone, Natalia; Magnano, Gabriel; Zapata, Laura; Abdala, Alejandro Ariel; Tarabla, Héctor D.; Peyrú, Maite; Caimi, Karina; Zumárraga, Martín José; Canal, A.M.; Cataldi, Angel

doi:10.4161/viru.27193

Cited by 20 publications

(19 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Moreover, results of the present study showed different sensitivity of diagnostic tests depending of the species of the MTC involved and the lesions caused, as it has been previously reported in previous studies (Buendia et al, 2013). Nevertheless, not only the species has been associated to the virulence but also the strain (Garbaccio et al, 2014), suggesting that differences observed between M. bovis and M. caprae in goats could be due to this fact since previous studies have described similar lesions in M. bovis and M. caprae infected goats (Quintas et al, 2010;Sanchez et al, 2011).…”

Section: Resultssupporting

confidence: 52%

Goats challenged with different members of the Mycobacterium tuberculosis complex display different clinical pictures

Bezos

Casal²,

Díez‐Delgado

et al. 2015

Veterinary Immunology and Immunopathology

View full text Add to dashboard Cite

Section: Resultssupporting

confidence: 52%

Goats challenged with different members of the Mycobacterium tuberculosis complex display different clinical pictures

Bezos

Casal²,

Díez‐Delgado

et al. 2015

Veterinary Immunology and Immunopathology

View full text Add to dashboard Cite

“…Na Europa, esse espoligotipo é frequentemente relatado na França (Haddad et al 2001), Espanha (Rodríguez et al 2010) e Portugal (Duarte et al 2008). Curiosamente, nesse estudo, observou-se que a maior parte dos isolados com esse espoligotipo (3/4) era proveniente de bovinos sem lesoes visíveis, o que pode sugerir baixa virulência (Garbaccio et al 2014), embora não se conhecesse o estágio de evolução da doença.…”

Section: Discussionunclassified

Identificação e genotipagem de Mycobacterium bovis em bovinos positivos no teste intradérmico para tuberculose em Mato Grosso do Sul

et al. 2015

View full text Add to dashboard Cite

Neste estudo, realizou-se genotipagem de isolados de Mycobacterium bovis, provenientes de amostras de tecidos de bovinos positivos no teste cervical comparativo (TCC) para tuberculose em Mato Grosso do Sul, por meio da técnica de spoligotyping. Tecidos de 13 bovinos positivos, oriundos de diferentes municípios do estado, foram cultivados em meio de Stonebrink. As colônias resultantes foram submetidas à coloração de Ziehl-Neelsen e todos os isolados apresentaram características tintoriais de BAAR. Os 13 isolados de BAAR foram identificados por PCR multiplex (mPCR). O gene hsp65 foi alvo para identificação de Mycobacterium spp, a sequência de inserção IS6110 foi alvo para identificação de complexo Mycobacterium tuberculosis (CMT) e a região rvd1rv2031c foi explorada para detecção de M. bovis. Os isolados micobacterianos foram genotipados pela técnica de spoligotyping. Dos 13 bovinos, sete tinham pelo menos uma lesão sugestiva de tuberculose em linfonodos retrofaríngeos, parotídeos e pulmonares ou no pulmão, e em seis não foram encontradas lesões visíveis sugestivas da doença. Na mPCR, 11/13 (84,6%) isolados foram positivos para Mycobacterium spp; 8/13 (61,5%) positivos para CMT e 7/13 (53,8%) positivos para M. bovis. Com base no spoligotyping, oito isolados de BAAR foram agrupados dentro de três diferentes agrupamentos de genótipos e uma amostra remanescente apresentou perfil único, sendo quatro isolados com padrão de espoligotipo SB0121, dois SB1145, dois SB0881 e um SB0140. A técnica de spoligotyping demonstrou que há diversidade genética entre os espoligotipos presentes no estado de Mato Grosso do Sul, embora predomine o perfil SB0121

show abstract

“…The adult female giraffe, which was found to be infected with SB0294 in this study (case 1), lived in the same nature reserve as the blue wildebeest infected with SB2200. Although it is unknown whether an increase in the number of circulating outbreak strains has any effect on the bovine TB disease dynamics in an already infected ecosystem, strain‐associated differences in virulence have been reported (Garbaccio et al, ). Therefore, enhanced spillover to other, especially endangered, wildlife species or neighbouring domestic animals cannot be ruled out as a possible consequence.…”

Section: Discussionmentioning

confidence: 99%

First detection ofMycobacterium bovisinfection in Giraffe (Giraffa camelopardalis) in the Greater Kruger National Park Complex: Role and implications

Hlokwe

Michel

Mitchel

et al. 2019

Transbound Emerg Dis

View full text Add to dashboard Cite

Bovine tuberculosis (bovine TB) caused by Mycobacterium bovis has become endemic in some wildlife populations in South Africa. The disease has been reported in 21 wildlife species in the country. In this study, we report M. bovis infection in two female giraffes (Giraffa camelopardalis) from two different nature reserves within the Greater Kruger National Park Complex (GKNPC). Mycobacterium bovis was isolated from tissue lesions consistent with macroscopic appearance of tuberculosis (TB) and confirmed by polymerase chain reactions (PCRs), targeting the RD4 region of difference on the genome of the isolates. Spoligotyping and variable number of tandem repeat (VNTR) typing revealed infection of one giraffe with a strain (SB0294) previously not detected in South Africa, while a resident M. bovis strain (SB0121) was detected from the other giraffe. Our work is first to report M. bovis infection in free‐ranging giraffes in South Africa. We have further demonstrated the existence of at least three genetically unrelated strains currently infecting wildlife species within the GKNPC. This finding suggests that the epidemiological situation of M. bovis within the GKNPC is not only driven by internal sources from its established endemic presence, but can be additionally fuelled by strains introduced from external sources. It further emphasizes that regular wildlife disease surveillance is an essential prerequisite for the timely identification of new pathogens or strains in ecospheres of high conservation value.

show abstract

Association between spoligotype-VNTR types and virulence ofMycobacterium bovisin cattle

Cited by 20 publications

References 36 publications

Goats challenged with different members of the Mycobacterium tuberculosis complex display different clinical pictures

Goats challenged with different members of the Mycobacterium tuberculosis complex display different clinical pictures

Identificação e genotipagem de Mycobacterium bovis em bovinos positivos no teste intradérmico para tuberculose em Mato Grosso do Sul

First detection ofMycobacterium bovisinfection in Giraffe (Giraffa camelopardalis) in the Greater Kruger National Park Complex: Role and implications

Contact Info

Product

Resources

About