Identificação e genotipagem de Mycobacterium bovis em bovinos positivos no teste intradérmico para tuberculose em Mato Grosso do Sul

Abstract

Neste estudo, realizou-se genotipagem de isolados de Mycobacterium bovis, provenientes de amostras de tecidos de bovinos positivos no teste cervical comparativo (TCC) para tuberculose em Mato Grosso do Sul, por meio da técnica de spoligotyping. Tecidos de 13 bovinos positivos, oriundos de diferentes municípios do estado, foram cultivados em meio de Stonebrink. As colônias resultantes foram submetidas à coloração de Ziehl-Neelsen e todos os isolados apresentaram características tintoriais de BAAR. Os 13 isolados de BAAR foram identificados por PCR multiplex (mPCR). O gene hsp65 foi alvo para identificação de Mycobacterium spp, a sequência de inserção IS6110 foi alvo para identificação de complexo Mycobacterium tuberculosis (CMT) e a região rvd1rv2031c foi explorada para detecção de M. bovis. Os isolados micobacterianos foram genotipados pela técnica de spoligotyping. Dos 13 bovinos, sete tinham pelo menos uma lesão sugestiva de tuberculose em linfonodos retrofaríngeos, parotídeos e pulmonares ou no pulmão, e em seis não foram encontradas lesões visíveis sugestivas da doença. Na mPCR, 11/13 (84,6%) isolados foram positivos para Mycobacterium spp; 8/13 (61,5%) positivos para CMT e 7/13 (53,8%) positivos para M. bovis. Com base no spoligotyping, oito isolados de BAAR foram agrupados dentro de três diferentes agrupamentos de genótipos e uma amostra remanescente apresentou perfil único, sendo quatro isolados com padrão de espoligotipo SB0121, dois SB1145, dois SB0881 e um SB0140. A técnica de spoligotyping demonstrou que há diversidade genética entre os espoligotipos presentes no estado de Mato Grosso do Sul, embora predomine o perfil SB0121

Molecular characterization of Mycobacterium bovis infection in cattle and buffalo in Amazon Region, Brazil

“…The spoligotype SB0121 is considered the most prevalent in several studies and the most frequent in Brazil (Zumárraga et al, ) and was observed in two (5.7%) buffaloes in the municipality of Autazes, as well as described in other studies, in the states of Bahia (Filho et al, ), Paraíba (Parreiras et al, ), Mato Grosso, Mato Grosso do Sul (Cazola et al, ), Goiás (Carvalho et al, ), Minas Gerais (Parreiras et al, ) and Rio Grande do Sul (Ramos et al, ). Outside of Brazil, SB0121 has been described in Colombia (Jojoa‐Jojoa et al, ), Argentina, Venezuela (Zumárraga et al, ), Mexico (Reyes et al, ), Portugal (Reis, ) and France (Hauer et al, ).…”

Isolation and identification of Mycobacterium bovis in bovines with positive reaction to the tuberculin test in the state of Paraíba, northeast Brazil

“…Furthermore, at necropsy, 21 (65.6%) animals presented lesions suggestive of tuberculosis. In fact, the recognition of macroscopic lesions associated with tuberculosis, especially during routine sanitary inspections at slaughterhouses, is an important tool for infection diagnosis, contributing to the identification of foci in herds (CAZOLA et al, 2015). In addition, this is one of PNCEBT's strategies of action in collaboration with the official sanitary inspection service, which is fundamental for warranting the supply of products posing low-risk to public health and, consequently, consumer protection (BIFFA et al, 2010;CAZOLA et al, 2015).…”

“…In fact, the recognition of macroscopic lesions associated with tuberculosis, especially during routine sanitary inspections at slaughterhouses, is an important tool for infection diagnosis, contributing to the identification of foci in herds (CAZOLA et al, 2015). In addition, this is one of PNCEBT's strategies of action in collaboration with the official sanitary inspection service, which is fundamental for warranting the supply of products posing low-risk to public health and, consequently, consumer protection (BIFFA et al, 2010;CAZOLA et al, 2015). In other countries such as the USA, Australia and Spain, where inspection services in slaughterhouses and programs for the eradication of tuberculosis are satisfactory, the prevalence of the disease was reduced (KANTOR; RITACCO, 2006;RUA-DOMENECH, 2006).…”

“…In Brazil, ALZAMORA FILHO et al (2014) reported lesions suggestive of tuberculosis in the pulmonary parenchyma, head and mediastinal lymph nodes in 75% (135/180) of findings in sanitary inspection. CAZOLA et al (2015) reported that, among 13 bovines with positive reaction to the tuberculin test, seven (53.8%) had at least one lesion suggestive of tuberculosis in retropharyngeal, parotid and pulmonary lymph nodes or in the lung, and six (46.2%) had no lesions suggestive of the disease. These results corroborate the findings of the present study and reinforce the role of the respiratory route in the transmission of mycobacteria.…”

“…Its diagnosis can be made by direct and indirect methods. Direct methods involve detection and identification of the etiologic agent in biological material, and the combination of isolation in culture medium and molecular identification with genotyping has contributed to a better understanding of the epidemiology of M. bovis infections, which provides greater efficiency to control programs (CAZOLA et al, 2015).…”