Soybean peroxidase immobilized on δ-FeOOH as new magnetically recyclable biocatalyst for removal of ferulic acid

Abstract

A significant enhancement in the catalytic performance due to enzymes immobilization is a great way to enhance the economics of biocatalytic processes. The soybean peroxidase (SP) immobilization under ferroxyte and the ferulic acid removal by the enzyme free and immobilized were investigated. The immobilization via silica-coated ferroxyte nanoparticles was effective, and immobilization yield of 39%. The scanning electron microscopy (SEM) images showed significant changes in the materials morphology. Substantial differences were observed in the particles' Fourier Transform Infrared (FTIR) spectra. The magnetic catalyst revealed a better performance than the free enzyme in the ferulic acid conversion, presenting a good V /K ratio when compared with the free enzyme. The reuse evaluated by ten cycles exhibited excellent recycling, remaining constant between the sixth and seventh cycles. The use of magnetic nanocatalyst becomes possible to eliminate the high operational costs, and complicated steps of the conventional enzymatic processes. Thus, a viable industrial route for the use of the enzyme as catalyst is possible.

Δ-FeOOH as Support for Immobilization Peroxidase: Optimization via a Chemometric Approach

“…The ∆-FeOOH nanoparticles were synthesized according to the modified procedure described by Tavares and collaborators [15]. Typically, 31.36 g of Fe(NH 4 ) 2 (SO 4 ) 2 •6H 2 O was dispersed into 200 mL of deionized water.…”

“…Accordingly, the iron hydroxyl oxide (∆-FeOOH) presents an excellent proposal as molecule immobilization, as the surface interaction of the enzyme is possible without requiring the addition of functional groups. Compared with other methods of enzyme immobilization technologies reported previously [15,16], this material as an enzymatic support can reduce the costs of the process, resulting in a low-cost magnetic nanocatalyst obtained.…”

“…Enzyme immobilization is an attractive technique to achieve reuse and stabilization of biocatalysts [11][12][13][14]. Different methods have investigated the enzymatic immobilization, being the adsorption frequently exploited [13,15,16], which involves covalent bonds embracing physical, chemical, and electrostatic interactions. Immobilization processes by covalent bonds are highlighted owing to the possibility of obtaining stable catalysts [17].…”

“…Iron hydroxyl oxide (FeOOH) has an attractive surface with the presence of hydroxyl groups (OH-), which may facilitate enzyme attachment. Besides, it exhibits unique characteristics (e.g., high surface area, magnetism, no-toxicity, and excellent stability) [15,20,21] and overcomes drawbacks regarding functionalization steps. Accordingly, the iron hydroxyl oxide (∆-FeOOH) presents an excellent proposal as molecule immobilization, as the surface interaction of the enzyme is possible without requiring the addition of functional groups.…”

Methane Activation by (n=0, 1, 2; m= 1, 2): Reactivity Parameters, Electronic Properties and Binding Energy Analysis

“…The growing overall need for energy brought about by the technological development of society has led to the development of researches aiming at safer and more economical energy sources . Natural gas (NG) appears as a promising alternative resource for energy supply owing to the discovery of vast reserves, low cost and by being a less polluting fuel and source of raw material and fuel generation for modern industry …”