Soybean Metabolites Regulated in Root Hairs in Response to the Symbiotic Bacterium<i>Bradyrhizobium japonicum</i>

Brechenmacher, Laurent; Lei, Zhentian; Libault, Marc; Findley, Seth D.; Sugawara, Masayuki; Sadowsky, Michael J.; Sumner, Lloyd W.; Stacey, Gary

doi:10.1104/pp.110.157800

Cited by 141 publications

(97 citation statements)

References 151 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The invasion of plant tissues toward the nodule requires the cells to traverse a stressful environment (43)(44)(45)(46), and it was observed that B. japonicum cells that are deficient in ␣-ketoglutarate dehydrogenase produce nodule primordia that abort at higher rates. This observation suggests that energy must be efficiently produced by the rhizobia to complete root infection (47).…”

Section: Discussionmentioning

confidence: 99%

Analysis of Two Polyhydroxyalkanoate Synthases in Bradyrhizobium japonicum USDA 110

Quelas

Mongiardini

Pérez-Giménez

et al. 2013

Journal of Bacteriology

View full text Add to dashboard Cite

bBradyrhizobium japonicum USDA 110 has five polyhydroxyalkanoate (PHA) synthases (PhaC) annotated in its genome: bll4360 (phaC1), bll6073 (phaC2), blr3732 (phaC3), blr2885 (phaC4), and bll4548 (phaC5). All these proteins possess the catalytic triad and conserved amino acid residues of polyester synthases and are distributed into four different PhaC classes. We obtained mutants in each of these paralogs and analyzed phaC gene expression and PHA production in liquid cultures. Despite the genetic redundancy, only phaC1 and phaC2 were expressed at significant rates, while PHA accumulation in stationary-phase cultures was impaired only in the ⌬phaC1 mutant. Meanwhile, the ⌬phaC2 mutant produced more PHA than the wild type under this condition, and surprisingly, the phaC3 transcript increased in the ⌬phaC2 background. A double mutant, the ⌬phaC2 ⌬phaC3 mutant, consistently accumulated less PHA than the ⌬phaC2 mutant. PHA accumulation in nodule bacteroids followed a pattern similar to that seen in liquid cultures, being prevented in the ⌬phaC1 mutant and increased in the ⌬phaC2 mutant in relation to the level in the wild type. Therefore, we used these mutants, together with a ⌬phaC1 ⌬phaC2 double mutant, to study the B. japonicum PHA requirements for survival, competition for nodulation, and plant growth promotion. All mutants, as well as the wild type, survived for 60 days in a carbon-free medium, regardless of their initial PHA contents. When competing for nodulation against the wild type in a 1:1 proportion, the ⌬phaC1 and ⌬phaC1 ⌬phaC2 mutants occupied only 13 to 15% of the nodules, while the ⌬phaC2 mutant occupied 81%, suggesting that the PHA polymer is required for successful competitiveness. However, the bacteroid content of PHA did not affect the shoot dry weight accumulation.

show abstract

Section: Discussionmentioning

confidence: 99%

Analysis of Two Polyhydroxyalkanoate Synthases in Bradyrhizobium japonicum USDA 110

Quelas

Mongiardini

Pérez-Giménez

et al. 2013

Journal of Bacteriology

View full text Add to dashboard Cite

show abstract

“…In order to understand the processes behind rhizobial infection and nodule development, a number of studies have identified genes, proteins, and metabolites regulated during the interaction between legume host species and rhizobia (Benedito et al 2008;Brechenmacher et al 2008Brechenmacher et al , 2009Brechenmacher et al , 2010Desbrosses et al 2005;Libault et al 2010a;Lim et al 2010;Lohar et al 2006;Oehrle et al 2008;Wan et al 2005). These studies have implicated a large number of genes, proteins, and metabolites involved in signal transduction, plant defense, carbon and nitrogen metabolism, and nutrient exchange during establishment of RNS.…”

mentioning

confidence: 99%

A Soybean Acyl Carrier Protein, GmACP, Is Important for Root Nodule Symbiosis

Wang¹,

Tóth²,

Tanaka³

et al. 2014

MPMI

Self Cite

View full text Add to dashboard Cite

Legumes (members of family Fabaceae) establish a symbiotic relationship with nitrogen-fixing soil bacteria (rhizobia) to overcome nitrogen source limitation. Single root hair epidermal cells serve as the entry point for bacteria to infect the host root, leading to development of a new organ, the nodule, which the bacteria colonize. In the present study, the putative role of a soybean acyl carrier protein (ACP), GmACP (Glyma18g47950), was examined in nodulation. ACP represent an essential cofactor protein in fatty acid biosynthesis. Phylogenetic analysis of plant ACP protein sequences showed that GmACP was classified in a legume-specific clade. Quantitative reverse-transcription polymerase chain reaction analysis demonstrated that GmACP was expressed in all soybean tissues but showed higher transcript accumulation in nodule tissue. RNA interference-mediated gene silencing of GmACP resulted in a significant reduction in nodule numbers on soybean transgenic roots. Fluorescent protein-labeled GmACP was localized to plastids in planta, the site of de novo fatty acid biosynthesis in plants. Analysis of the fatty acid content of root tissue silenced for GmACP expression, as determined by gas chromatography-mass spectrometry, showed an approximately 22% reduction, specifically in palmitic and stearic acid. Taken together, our data provide evidence that GmACP plays an important role in nodulation.

show abstract

“…Over the last decade, various large-scale approaches such as transcriptomics, proteomics, and metabolomics, have been used to dissect the legume-rhizobia symbiosis (27)(28)(29). However, most of these studies looked at relatively late responses to rhizobia or NF and unfortunately were not integrated.…”

mentioning

confidence: 99%

Rapid Phosphoproteomic and Transcriptomic Changes in the Rhizobia-legume Symbiosis

Rose

Venkateshwaran

Volkening

et al. 2012

Molecular & Cellular Proteomics

110

135

View full text Add to dashboard Cite

Symbiotic associations between legumes and rhizobia usually commence with the perception of bacterial lipochitooligosaccharides, known as Nod factors (NF), which triggers rapid cellular and molecular responses in host plants. We report here deep untargeted tandem mass spectrometry-based measurements of rapid NF-induced changes in the phosphorylation status of 13,506 phosphosites in 7739 proteins from the model legume Medicago truncatula. To place these phosphorylation changes within a biological context, quantitative phosphoproteomic and RNA measurements in wild-type plants were compared with those observed in mutants, one defective in NF perception (nfp) and one defective in downstream signal transduction events (dmi3). Our study quantified the early phosphorylation and transcription dynamics that are specifically associated with NF-signaling, confirmed a dmi3-mediated feedback loop in the pathway, and suggested "cryptic" NF-signaling pathways, some of them being also involved in the response to symbiotic arbuscular mycorrhizal fungi. Legumes have the ability to form a very efficient symbiotic association with rhizobia to meet their nitrogen demand. This results in root nodule formation, inside which the rhizobia can fix atmospheric nitrogen efficiently and transfer it to the plants, in exchange for a carbon source. This interaction is often characterized by a high level of host specificity and generally requires an exchange of diffusible signals between legumes and rhizobia. Flavonoids and isoflavonoids present in the legume root exudates induce the expression of nod genes in rhizobia, and are responsible for the production and secretion of bacterial lipochitooligosaccharides (LCOs), known as Nod factors (NF) 1 (1, 2). NF are generally required for rhizobial infection and nodule development. Mere application of purified NF at low concentrations (10 Ϫ8 to 10 Ϫ12 M) is sufficient to trigger responses in host plants similar to those elicited by the rhizobia themselves (2, 3). Responses are elicited in root cells within a few seconds to minutes after NF application and include changes in ion fluxes across the plasma membrane, as well as accumulation of reactive oxygen species (4, 5). After 15-20 min, oscillations of the nuclear and perinuclear calcium concentration (calcium spiking) are initiated and trigger the expression of early nodulin genes (3). Within the first hour, legume root hairs undergo a cytoplasmic disorganization ,which leads to a transient swelling (6), followed by root hair deformations (7).Forward and reverse genetic approaches, in the model legumes Medicago truncatula (Medicago) and Lotus japonicus (Lotus), have identified several components controlling these events (Fig. 1). NF are perceived with high specificity by LysM receptor-like kinases residing on the plasma membrane. These kinases include nod factor perception (NFP), an essential component of a signaling receptor necessary for early responses to NF and rhizobial infection (8, 9). Mutants in NFP are affected in all known cellular respons...

show abstract

Soybean Metabolites Regulated in Root Hairs in Response to the Symbiotic BacteriumBradyrhizobium japonicum

Cited by 141 publications

References 151 publications

Analysis of Two Polyhydroxyalkanoate Synthases in Bradyrhizobium japonicum USDA 110

Analysis of Two Polyhydroxyalkanoate Synthases in Bradyrhizobium japonicum USDA 110

A Soybean Acyl Carrier Protein, GmACP, Is Important for Root Nodule Symbiosis

Rapid Phosphoproteomic and Transcriptomic Changes in the Rhizobia-legume Symbiosis

Contact Info

Product

Resources

About