Soybean Isoflavones Eliminate Nifedipine-Induced Flushing of Tail Skin in Ovariectomized Mice

Abstract

Abstract. Hot flushes are one of the most frequent symptoms in menopausal women. We investigated effect of soybean isoflavones (Soyaflavone HG) on nifedipine-induced flushing in ovariectomized mice. Ovariectomy markedly aggravated nifedipine-induced increase in tail skin temperature. Soyaflavone HG (10 mg / kg, p.o., once a day for 5 days) inhibited nifedipineinduced flushing in ovariectomized mice. The inhibitory effect of Soyaflavone HG was significantly reversed by an estrogen-receptor antagonist, ICI 182,780, suggesting that Soyaflavone HG prevents nifedipine-induced flushing partially through estrogen receptors. We presented the experimental evidence suggesting that soybean isoflavones including Soyaflavone HG have the benefits for menopausal hot flushes.

Dietary Supplementation with Isoflavones Prevents Muscle Wasting in Tumor-Bearing Mice

“…The ␣-starch content of the isofl avone diet was reduced to adjust for the composition of other nutrients, and comprised a normal diet (based on AIN-93M) mixed with soya fl avone HG (0.4% w/w; Fuji Oil Co., Osaka, Japan). The content of isofl avones in soya fl avone HG was 40.74% and the specifi c components have been described in a previous report (18). The right hindlimb skeletal muscles, such as the tibialis anterior (TA), extensor digitorum longus (EDL), gastrocnemius (GA), and soleus (So) muscles, were isolated at the time of sacrifi ce.…”

S-equol and the fermented soy product SE5-OH containing S-equol similarly decrease ovariectomy-induced increase in rat tail skin temperature in an animal model of hot flushes

“…One possible mechanism is working through an estrogen-like mechanism because previous work has shown that the ER antagonist ICI-182,780 inhibits the effects of isoflavones on TST. 4 However, ICI-182,780 only partially inhibits the effect of isoflavones. 4 Moreover, a previous report found that S-equol transactivates ER-> and ER-A 4,000-and 600-fold, respectively, less than estradiol.…”

“…4 However, ICI-182,780 only partially inhibits the effect of isoflavones. 4 Moreover, a previous report found that S-equol transactivates ER-> and ER-A 4,000-and 600-fold, respectively, less than estradiol. 3 Therefore, ER-independent mechanisms may also be involved in the TST-reducing effect of S-equol.…”

“…4,5 Consequently, these soybean constituents are being studied as possible alternatives to conventional hormone therapy for the alleviation of hot flushes as well as for other diseases common to postmenopausal women such as osteoporosis and hyperlipidemia. 6,7 Hot flushes, one of the most troublesome symptoms of menopause, occur in a large percentage of climacteric women, although prevalence varies among countries and ethnicities.…”

Inhibition of mRNA Maturation by Compounds Which Have a Flavonoid Skeleton

“…Because a limited number of inhibitors are available to study the diverse mechanisms of mRNA processing, the identification of a new compound could lead to the discovery of a novel mRNA processing mechanism. The component composition of SHG is already shown previously [40]. Among them, the major constituents of the isoflavone fraction are daidzin, genistin and glycitin, and their derivatives including aglycone forms.…”