Soyasaponins: A New Class of Root Exudates in Soybean (Glycine max)

Tsuno, Yuhei; Fujimatsu, Teruhisa; Endo, Kiyoshi; Sugiyama, Akifumi; Yazaki, Kazufumi

doi:10.1093/pcp/pcx192

Cited by 63 publications

(85 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…All fragmentation peaks in both -ve and +ve ion modes are illustrated in consecutive losses of H 2 O molecules. These data were in full matching with previous literature [16], revealing that the aglycone part most probably is soyasapogenol Bb. So the whole compound could be identified as the triterpenoid oligosaccharide (triterpenoid glycosides) saponin of the oleanane type; Soyasaponin Bb; 22,24-dihydroxyolean-12-en-3-yl-rhamnopyranosyl-(1->2)-beta-Dgalactopyranosyl-(1->2)-beta glucouronic acid.…”

Section: Investigation Of Chemical Compounds In Methanol Leaves Fractionsupporting

confidence: 89%

Chemical Characterization of Constituents Isolated from Hamelia patens and Investigating Its Cytotoxic Activity

et al. 2019

View full text Add to dashboard Cite

S RB cytotoxicity assay was performed for different extracts of Hamelia patens Jacq. family Rubiaceae; crude flowers (CF), crude leaves (CL), chloroform (Chl.L) and methanol (Me.L) fractions from leaves against liver (HepG-2) and breast (MCF-7) human carcinoma cell lines. Results demonstrated potent cytotoxic action against HEPG-2 for CF, Chl.L and Me.L with IC 50 = 47, 30 and 44.4 µg/mL, respectively. On the other hand, CF, CL, Chl.L and Me.L had exerted powerful effect against MCF-7 with IC 50 = 23.8, 25.5, 17.7 and 64 µg/mL, respectively. Chromatographic investigation of Me.L , resulted in the isolation and spectroscopic identification of rutin (1), isoquercetin (2) and soyasaponin Bb (3), which was isolated for the first time from the plant leaves and was identified by UPLC/ITMS/MS analysis. Comparative study for using ordinary method versus green methods of extraction was also inspected.

show abstract

Section: Investigation Of Chemical Compounds In Methanol Leaves Fractionsupporting

confidence: 89%

Chemical Characterization of Constituents Isolated from Hamelia patens and Investigating Its Cytotoxic Activity

et al. 2019

View full text Add to dashboard Cite

show abstract

“…S4). Furthermore, Fabaceae triterpene saponins likely play important roles in the rhizosphere, as reported in previous studies; increased saponin accumulation enhanced nodulation [16] and soyasaponins were the major component of root exudates [37]. These observations suggest that members of group 2 affect biological interactions in the rhizosphere through modulation of soyasaponin production.…”

Section: Discussionsupporting

confidence: 77%

Insights into the Diversification of Subclade IVa bHLH Transcription Factors in Fabaceae

Suzuki¹,

Seki²,

Muranaka³

2020

Preprint

View full text Add to dashboard Cite

Background: Fabaceae plants appear to contain larger numbers of subclade IVa basic-helix-loop-helix (bHLH) transcription factors than other plant families, and some members of this subclade have been identified as saponin biosynthesis regulators. We aimed to systematically elucidate the diversification of this subclade and obtain insights into the evolutionary history of saponin biosynthesis regulation in Fabaceae.Results: In this study, we collected sequences of subclade IVa bHLH proteins from 40 species, including fabids and other plants, and found greater numbers of subclade IVa bHLHs in Fabaceae. We confirmed conservation of the bHLH domain, C-terminal ACT-like domain, and exon-intron organisation among almost all subclade IVa members in model legumes, supporting the results of our classification. Phylogenetic tree-based classification of subclade IVa revealed the presence of three different groups. Interestingly, most Fabaceae subclade IVa bHLHs fell into group 1, which contained all legume saponin biosynthesis regulators identified to date. These observations support the co-occurrence and Fabaceae-specific diversification of saponin biosynthesis regulators. Comparing the expression of orthologous genes in Glycine max, Medicago truncatula, and Lotus japonicus, orthologues of MtTSAR1 (the first identified soyasaponin biosynthesis regulatory transcription factor) were not expressed in the same tissues, suggesting that group 1 members have gained different expression patterns and contributions to saponin biosynthesis during their duplication and divergence. On the other hand, groups 2 and 3 possessed fewer members, and their phylogenetic relationships and expression patterns were highly conserved, indicating that their activities may be conserved across Fabaceae.Conclusions: This study suggests subdivision and diversification of subclade IVa bHLHs in Fabaceae plants. The results will be useful for candidate selection of unidentified saponin biosynthesis regulators. Furthermore, the functions of groups 2 and 3 members are interesting targets for clarifying the evolution of subclade IVa bHLH transcription factors in Fabaceae.

show abstract

“…Plant root exudates play an essential role in resource competition and chemical interference [15]. Tsuno et al [58] reported that soybean roots secrete large amounts of soyasaponins (a new type of root exudate) during the vegetative emergence (VE) stage of growth. Liu et al [59] found that soybean root exudates trigger early symbiotic associations between Bacillus diazoefficiens and soybean.…”

Section: Root Exudationmentioning

confidence: 99%

The Interactions of Rhizodeposits with Plant Growth-Promoting Rhizobacteria in the Rhizosphere: A Review

2019

View full text Add to dashboard Cite

Rhizodeposits, root exudates, and root border cells are vital components of the rhizosphere that significantly affect root colonization capacity and multiplication of rhizosphere microbes, as well as secretion of organic bioactive compounds. The rhizosphere is an ecological niche, in which beneficial bacteria compete with other microbiota for organic carbon compounds and interact with plants through root colonization activity to the soil. Some of these root-colonizing beneficial rhizobacteria also colonize endophytically and multiply inside plant roots. In the rhizosphere, these components contribute to complex physiological processes, including cell growth, cell differentiation, and suppression of plant pathogenic microbes. Understanding how rhizodeposits, root exudates, and root border cells interact in the rhizosphere in the presence of rhizobacterial populations is necessary to decipher their synergistic role for the improvement of plant health. This review highlights the diversity of plant growth-promoting rhizobacteria (PGPR) genera, their functions, and the interactions with rhizodeposits in the rhizosphere.Agriculture 2019, 9, 142 2 of 13 in the rhizosphere [12,13], and these interactions that influence plant growth and crop yields [14] can be root-root, root-insect, and root-microbe interactions [15]. The role of the rhizosphere is pivotal for plant growth-promotion, nutrition, and crop quality [16] because of the importance of plant-microbe interactions in the rhizosphere carbon sequestration, nutrient cycling, and ecosystem functioning [17]. In addition, the rhizosphere is where plant roots communicate with beneficial rhizobacteria for energy and nutrition. Plant growth-promoting rhizobacteria (PGPR) may affect plant growth, development, and disease suppression by one or more direct or indirect mechanisms. Bacterial genera such as Bacillus and Pseudomonas have been extensively studied and utilized as biocontrol agents, biofertilizers, and also have been shown to trigger induced systemic resistance (ISR) [18][19][20][21][22][23][24]. In this review, we discuss the importance, functions, and effects of root-derived organic molecules secreted in the rhizosphere and their interactions with plant growth-promoting rhizobacteria (PGPR) for enhancing plant growth and biological control of plant pathogens. PGPR Diversity in the RhizosphereThe plant rhizosphere contains diverse rhizobacterial species with the potential to enhance plant growth and biological control activity. PGPR genera present in the rhizosphere include Agrobacterium,

show abstract

Soyasaponins: A New Class of Root Exudates in Soybean (Glycine max)

Cited by 63 publications

References 36 publications

Chemical Characterization of Constituents Isolated from Hamelia patens and Investigating Its Cytotoxic Activity

Chemical Characterization of Constituents Isolated from Hamelia patens and Investigating Its Cytotoxic Activity

Insights into the Diversification of Subclade IVa bHLH Transcription Factors in Fabaceae

The Interactions of Rhizodeposits with Plant Growth-Promoting Rhizobacteria in the Rhizosphere: A Review

Contact Info

Product

Resources

About