Chemical Characterization of Constituents Isolated from Hamelia patens and Investigating Its Cytotoxic Activity

Abstract

S RB cytotoxicity assay was performed for different extracts of Hamelia patens Jacq. family Rubiaceae; crude flowers (CF), crude leaves (CL), chloroform (Chl.L) and methanol (Me.L) fractions from leaves against liver (HepG-2) and breast (MCF-7) human carcinoma cell lines. Results demonstrated potent cytotoxic action against HEPG-2 for CF, Chl.L and Me.L with IC 50 = 47, 30 and 44.4 µg/mL, respectively. On the other hand, CF, CL, Chl.L and Me.L had exerted powerful effect against MCF-7 with IC 50 = 23.8, 25.5, 17.7 and 64 µg/mL, respectively. Chromatographic investigation of Me.L , resulted in the isolation and spectroscopic identification of rutin (1), isoquercetin (2) and soyasaponin Bb (3), which was isolated for the first time from the plant leaves and was identified by UPLC/ITMS/MS analysis. Comparative study for using ordinary method versus green methods of extraction was also inspected.

The research on conventional medicine: Hamelia patens

“…Maii Abdelnaby Ismail Maamoun et al (2019) [1] The SRB assay was used to determine the cytotoxicity of several extracts from the Rubiaceae family, which includes Hamelia patens Jacq. Human cancer cell lines HepG-2 and MCF-7 were evaluated against crude fractions of several plant parts, including crude flowers (CF), crude leaves (CL), chloroform (Chl.…”

The Ultrasound-Assisted Extraction of Polyphenols from Mexican Firecracker (Hamelia patens Jacq.): Evaluation of Bioactivities and Identification of Phytochemicals by HPLC-ESI-MS

“…It has been reported to possess several bioactivities in vitro and in vivo, including antihemorrhagic [6], hepatoprotective, antioxidant [7], antihyperglycemic [8], and antibacterial [5] properties, among others. The tested extracts contained phytomolecules such as epicatechin, chlorogenic acid [8], rutin, isoquercetin and soyasaponin Bb, which were first identified in the leaves Molecules 2022, 27, 8845 2 of 15 of the plant [9]. However, the obtention of bioactive compounds depends mostly on the extraction method and the solvent used.…”