Soy yogurt using Lactobacillus plantarum 200655 and fructooligosaccharides: neuroprotective effects against oxidative stress

Choi, Ga-Hyun; Bock, Hyun-Ji; Lee, Na‐Kyoung; Paik, Hyun‐Dong

doi:10.1007/s13197-022-05575-1

Cited by 7 publications

(3 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…MTT assay was conducted to evaluate cytotoxicity of CM using the method by Choi et al . [ 26 ]. SH-SY5Y cells were incubated in 96-well culture plates (1 × 10 5 cells/well) for 24 h. Then, LAB–CM was added.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Neuroprotective Effects of Heat-Killed Levilactobacillus brevis KU15152 on H₂O₂-Induced Oxidative Stress

Bock

Lee

Paik

2023

J. Microbiol. Biotechnol.

Self Cite

View full text Add to dashboard Cite

This study proposed to demonstrate the neuroprotective effects of heat-killed Levilactobacillus brevis KU15152. Heat-killed L. brevis KU15152 showed antioxidant activity similar to that of Lacticaseibacillus rhamnosus GG, in terms of radical scavenging activity. To evaluate the neuroprotective effects, conditioned medium (CM) obtained by incubating heat-killed bacteria in intestinal cells (HT-29) was used through gut-brain axis. CM from L. brevis KU15152 protected neuroblastoma cells (SH-SY5Y) against H 2 O 2 -induced oxidative stress. Pretreatment with CM significantly alleviated the morphological changes induced by H 2 O 2 . Heat-killed L. brevis KU15152 showed an increased brainderived neurotrophic factor (BDNF) expression in HT-29 cells. L. brevis KU15152-CM remarkably downregulated the Bax/Bcl-2 ratio, while upregulating the expression of BDNF and tyrosine hydroxylase (TH) in SH-SY5Y cells. Furthermore, L. brevis KU15152-CM reduced caspase-3 activity following H 2 O 2 treatment. In conclusion, L. brevis KU15152 can be potentially used as food materials to avoid neurodegenerative diseases.

show abstract

Section: Methodsmentioning

confidence: 99%

“…Relative gene expression was measured by Choi et al . [ 26 ]. To measure the expression of BDNF in intestinal cells, HT-29 cells were inoculated in 6-well culture plates at a density of 1 × 10 6 cells/well and incubated for 5 d. The HT-29 cells were incubated with heat-killed LAB (1 × 10 8 CFU/well) for 24 h.…”

Section: Methodsmentioning

confidence: 99%

Neuroprotective Effects of Heat-Killed Levilactobacillus brevis KU15152 on H₂O₂-Induced Oxidative Stress

Bock

Lee

Paik

2023

J. Microbiol. Biotechnol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…The DPPH radical scavenging activity was determined using a modi ed method described by Song et al [17]. In brief, a mixture of 500 µL of 0.1 mM DPPH solution (in 99% ethanol) and an equal volume of paraprobiotic sample (9 log CFU/mL) was incubated for 30 min in the dark.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant and immunostimulatory effect of heat-treated paraprobiotics Latilactobacillus sakei KU15041 and Latilactobacillus curvatus KU15003

Hyun,

Woo,

Kim

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

The lactic acid bacteria, including Latilactobacillus sakei and Latilactobacillus curvatus, have been widely studied for their preventive and therapeutic effects. In this study, the underlying mechanism of action for the antioxidant and immunostimulatory effects of two strains of heat-treated paraprobiotics was examined. Heat-treated Lact. sakei KU15041 and Lact. curvatus KU15003 showed higher radical scavenging activity in both the 2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 2,2-diphenyl-1-picryl-hydrazyl (DPPH) assays than the commercial probiotic strain LGG. In addition, treatment with these two strains exhibited immunostimulatory effects in RAW 264.7 macrophages, with Lact. curvatus KU15003 showing a slightly higher effect. Additionally, they promoted phagocytosis and NO production in RAW 264.7 cells without any cytotoxicity. Moreover, the expression of tumor necrosis factor-α, interleukin (IL)-1β, and IL-6 was upregulated. These strains resulted in an increased expression of inducible nitric oxide synthase and cyclooxygenase-2. Moreover, the nuclear factor-κB and mitogen-activated protein kinase signaling pathways were stimulated by these strains. These findings suggest the potential of using Lact. sakei KU15041 and Lact. curvatus KU15003 in food or by themselves as probiotics with antioxidant and immune-enhancing properties.

show abstract

Paraprobiotics: definition, manufacturing methods, and functionality

Lee

Kang

Paik

2023

Food Sci Biotechnol

View full text Add to dashboard Cite

Soy yogurt using Lactobacillus plantarum 200655 and fructooligosaccharides: neuroprotective effects against oxidative stress

Cited by 7 publications

References 33 publications

Neuroprotective Effects of Heat-Killed Levilactobacillus brevis KU15152 on H₂O₂-Induced Oxidative Stress

Neuroprotective Effects of Heat-Killed Levilactobacillus brevis KU15152 on H₂O₂-Induced Oxidative Stress

Antioxidant and immunostimulatory effect of heat-treated paraprobiotics Latilactobacillus sakei KU15041 and Latilactobacillus curvatus KU15003

Paraprobiotics: definition, manufacturing methods, and functionality

Contact Info

Product

Resources

About

Soy yogurt using Lactobacillus plantarum 200655 and fructooligosaccharides: neuroprotective effects against oxidative stress

Cited by 7 publications

References 33 publications

Neuroprotective Effects of Heat-Killed Levilactobacillus brevis KU15152 on H2O2-Induced Oxidative Stress

Neuroprotective Effects of Heat-Killed Levilactobacillus brevis KU15152 on H2O2-Induced Oxidative Stress

Antioxidant and immunostimulatory effect of heat-treated paraprobiotics Latilactobacillus sakei KU15041 and Latilactobacillus curvatus KU15003

Paraprobiotics: definition, manufacturing methods, and functionality

Contact Info

Product

Resources

About

Neuroprotective Effects of Heat-Killed Levilactobacillus brevis KU15152 on H₂O₂-Induced Oxidative Stress

Neuroprotective Effects of Heat-Killed Levilactobacillus brevis KU15152 on H₂O₂-Induced Oxidative Stress