Neuroprotective Effects of Heat-Killed Levilactobacillus brevis KU15152 on H2O2-Induced Oxidative Stress

Bock, Hyun-Ji; Lee, Na‐Kyoung; Paik, Hyun‐Dong

doi:10.4014/jmb.2304.04045

Cited by 5 publications

(1 citation statement)

References 45 publications

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“…Based on these preliminary in vivo findings, we aimed to further investigate the potential benefits of a probiotic mix containing the same strains found in Acronelle ® . To achieve this, we used an in vitro model of HT29 colorectal carcinoma cells, which serve as a well-established and widely utilized model for investigating various aspects of the human intestinal tract, particularly the intricate interactions between the host and microbial components [24][25][26]. We treated HT29 cells with the probiotics mix, either alone or in combination with hydrogen peroxide, for 24 h (probiotics mix 24 h, H 2 O 2 24 h, and probiotics mix and H 2 O 2 24 h synchronously).…”

Section: Introductionmentioning

confidence: 99%

Nanovesicular Mediation of the Gut–Brain Axis by Probiotics: Insights into Irritable Bowel Syndrome

Santonocito,

Paladino,

Vitale

et al. 2024

Biology

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Background: Dysbiosis, influenced by poor diet or stress, is associated with various systemic diseases. Probiotic supplements are recognized for stabilizing gut microbiota and alleviating gastrointestinal issues, like irritable bowel syndrome (IBS). This study focused on the tryptophan pathways, which are important for the regulation of serotonin levels, and on host physiology and behavior regulation. Methods: Nanovesicles were isolated from the plasma of subjects with chronic diarrhea, both before and after 60 days of consuming a probiotic mix (Acronelle®, Bromatech S.r.l., Milan, Italy). These nanovesicles were assessed for the presence of Tryptophan 2,3-dioxygenase 2 (TDO 2). Furthermore, the probiotics mix, in combination with H2O2, was used to treat HT29 cells to explore its cytoprotective and anti-stress effect. Results: In vivo, levels of TDO 2 in nanovesicles were enhanced in the blood after probiotic treatment, suggesting a role in the gut–brain axis. In the in vitro model, a typical H2O2-induced stress effect occurred, which the probiotics mix was able to recover, showing a cytoprotective effect. The probiotics mix treatment significantly reduced the heat shock protein 60 kDa levels and was able to preserve intestinal integrity and barrier function by restoring the expression and redistribution of tight junction proteins. Moreover, the probiotics mix increased the expression of TDO 2 and serotonin receptors. Conclusions: This study provides evidence for the gut–brain axis mediation by nanovesicles, influencing central nervous system function.

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Section: Introductionmentioning

confidence: 99%

Nanovesicular Mediation of the Gut–Brain Axis by Probiotics: Insights into Irritable Bowel Syndrome

Santonocito,

Paladino,

Vitale

et al. 2024

Biology

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Biofortification, metabolomic profiling and quantitative analysis of vitamin B₁₂ enrichment in guava juice via lactic acid fermentation using Levilactobacillus brevis strain KU15152

Rastogi,

Singh,

Shaida

et al. 2024

J Sci Food Agric

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BACKGROUNDChemical fortification and dose supplementation of vitamin B12 are widely implemented to combat deficiency symptoms. However, in situ, fortification of vitamin B12 in food matrixes can be a promising alternative to chemical fortification. The present study aimed to produce vitamin B12‐rich, probiotic guava juice fermented with Levilactobacillus brevis strain KU15152. Pasteurized fresh guava juice was inoculated with 7.2 log CFU mL−1 L. brevis strain KU15152 and incubated for 72 h at 37 °C anaerobically. The antioxidants, total phenolic compounds, vitamin B12 production, sugars, organic acids, pH and viable count were analyzed at 24, 48 and 72 h of incubation. The fermented juice was stored at 4 °C, and the changes in its functional properties were analyzed at 7‐day intervals up to 28 days of storage.RESULTSDuring fermentation, the bacteria cell count was increased from 7.01 ± 0.06 to 9.76 ± 0.42 log CFU mL−1 after 72 h of fermentation and was decreased to 6.94 ± 0.34 CFU mL−1 during storage at 4 °C after 28 days. The pH, total soluble solids, crude fiber, citric acid and total sugars decreased, while titratable acidity, total protein, antioxidants, phenolic compounds and lactic acid contents increased during fermentation. The fermented guava juice exhibited higher 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH) and 2,2′‐azino‐bis‐(3‐ethylbenzothiazoline‐6‐sulfonic acid (ABTS)) radical scavenging activities (85.97% and 75.97%, respectively) at 48 h of fermentation. The concentration of active vitamin B12 in the sample reached 109.5 μg L−1 at 72 h of fermentation. However, this concentration gradually decreased to 70.2 μg L−1 during the storage period. During storage for 28 days at 4 °C, both the fermented and control guava juices exhibited a decline in antioxidant and phenolic compound concentrations. Furthermore, the addition of 20% honey and guava flavor enhanced the organoleptic properties and acceptability of fermented guava juice.CONCLUSIONThe value‐added fermented guava juice could be a novel functional food product to combat vitamin B12 deficiency. © 2024 The Author(s). Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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Opening avenues for treatment of neurodegenerative disease using post-biotics: Breakthroughs and bottlenecks in clinical translation

Bashir,

Alam,

Khandale

et al. 2024

Ageing Research Reviews

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Neuroprotective Effects of Heat-Killed Levilactobacillus brevis KU15152 on H₂O₂-Induced Oxidative Stress

Cited by 5 publications

References 45 publications

Nanovesicular Mediation of the Gut–Brain Axis by Probiotics: Insights into Irritable Bowel Syndrome

Nanovesicular Mediation of the Gut–Brain Axis by Probiotics: Insights into Irritable Bowel Syndrome

Biofortification, metabolomic profiling and quantitative analysis of vitamin B₁₂ enrichment in guava juice via lactic acid fermentation using Levilactobacillus brevis strain KU15152

Opening avenues for treatment of neurodegenerative disease using post-biotics: Breakthroughs and bottlenecks in clinical translation

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Neuroprotective Effects of Heat-Killed Levilactobacillus brevis KU15152 on H2O2-Induced Oxidative Stress

Cited by 5 publications

References 45 publications

Nanovesicular Mediation of the Gut–Brain Axis by Probiotics: Insights into Irritable Bowel Syndrome

Nanovesicular Mediation of the Gut–Brain Axis by Probiotics: Insights into Irritable Bowel Syndrome

Biofortification, metabolomic profiling and quantitative analysis of vitamin B12 enrichment in guava juice via lactic acid fermentation using Levilactobacillus brevis strain KU15152

Opening avenues for treatment of neurodegenerative disease using post-biotics: Breakthroughs and bottlenecks in clinical translation

Contact Info

Product

Resources

About

Neuroprotective Effects of Heat-Killed Levilactobacillus brevis KU15152 on H₂O₂-Induced Oxidative Stress

Biofortification, metabolomic profiling and quantitative analysis of vitamin B₁₂ enrichment in guava juice via lactic acid fermentation using Levilactobacillus brevis strain KU15152