Soxhlet extraction of phenolic compounds from Vernonia cinerea leaves and its antioxidant activity

Alara, Oluwaseun Ruth; Nour, A. H.; Ukaegbu, Chinonso Ishmael

doi:10.1016/j.jarmap.2018.07.003

Cited by 127 publications

(100 citation statements)

References 16 publications

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“…). The broad band at 3343.61 cm −1 signifies a hydroxyl group, which indicates the presence of phenolic compounds in the extract . Sharp bands at 2977.28 and 2901.10 cm −1 correspond to aromatic CH stretching frequencies.…”

Section: Resultsmentioning

confidence: 99%

Optimizing Microwave‐Assisted Extraction Conditions to Obtain Phenolic‐Rich Extract from Chromolaena odorata Leaves

2019

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Phenolic compounds are secondary metabolites that are mainly responsible for different pharmacological activities of plant extracts. In this study, microwaveassisted extraction (MAE) variables were optimized for the extraction of phenolic compounds from Chromolaena odorata leaves by using a Box-Behnken design of response surface methodology. The concentration of polyphenols and functional characteristics of the extracts were identified by using liquid chromatography quadrupolar time-of-flight mass spectrometry and FTIR analyses, respectively. The obtained results indicated the MAE conditions for the optimal recoveries of the total phenolic content and total flavonoid content from C. odorata leaves. The results reveal that the C. odorata leaf is enriched with phenolic compounds.

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Section: Resultsmentioning

confidence: 99%

Optimizing Microwave‐Assisted Extraction Conditions to Obtain Phenolic‐Rich Extract from Chromolaena odorata Leaves

2019

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“…Various extractive methods have been proposed, including maceration, percolation, hydro-distillation, boiling, reflux, soaking, and soxhlet [32]. Soxhlet was the most commonly used method for the extraction of flavonoids due to its simplicity and ease of maintenance, low cost, and lower solvent content compared to other methods such as soaking, boiling, or maceration [14,33,34].…”

Section: Conventional Methodsmentioning

confidence: 99%

“…Various solvents such as ethanol, methanol, benzene, chloroform, ethyl acetate, etc. have been tested in this extraction method to compare the effect on extraction yields [15,32].…”

Section: Conventional Methodsmentioning

confidence: 99%

Conventional and Emerging Extraction Processes of Flavonoids

et al. 2020

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Flavonoids are a group of plant constituents called phenolic compounds and correspond to the nonenergy part of the human diet. Flavonoids are found in vegetables, seeds, fruits, and beverages such as wine and beer. Over 7000 flavonoids have been identified and they have been considered substances with a beneficial action on human health, particularly of multiple positive effects because of their antioxidant and free radical scavenging action. Although several studies indicate that some flavonoids have provident actions, they occur only at high doses, confirming in most investigations the existence of anti-inflammatory effects, antiviral or anti-allergic, and their protective role against cardiovascular disease, cancer, and various pathologies. Flavonoids are generally removed by chemical methods using solvents and traditional processes, which besides being expensive, involve long periods of time and affect the bioactivity of such compounds. Recently, efforts to develop biotechnological strategies to reduce or eliminate the use of toxic solvents have been reported, reducing processing time and maintaining the bioactivity of the compounds. In this paper, we review, analyze, and discuss methodologies for biotechnological recovery/extraction of flavonoids from agro-industrial residues, describing the advances and challenges in the topic.

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“…Total flavonoid content in the C. odorata leaf extract was determined using the procedure used in a previous study [15]. Then, TFC was calculated using Eq.…”

Section: Evaluation Of Total Flavonoid Contentmentioning

confidence: 99%

“…From the obtained results, the interactions between irradiation time and microwave power, irradiation time and ethanol concentration greatly contributed to the yields of TPC and TFC. It has been reported that solvent concentration, irradiation time and microwave power level are the main MAE variable that determines the quantity and quality of recovery yields from plant materials [9,[14][15]. Thus, the interactions between these three MAE factors play important role in recovering TPC and TFC from C. odorata leaves.…”

Section: Influence Of Mae Parameters On the Tpc And Tfc Yields From Cmentioning

confidence: 99%

Screening of Microwave-Assisted-Batch Extraction Parameters for Recovering Total Phenolic and Flavonoid Contents from <i>Chromolaena odorata</i> Leaves through Two-Level Factorial Design

2019

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Microwave-assisted extraction (MAE) of phenolic compounds from Chromolaena odorata leaves was investigated using one-factor-at-a-time (OFAT) and two-level factorial design. The MAE parameters studied were irradiation time (A: 1–5 min); microwave power level (B: 400-800 W); extraction temperature (C: 60–80 °C); solvent/feed ratio (D: 8:1–14:1 mL/g); and ethanol concentration (E: 20–60% v/v). The optima yields of TPC and TFC were 56.13 mg GAE/g d.w. and 44.78 mg QE/g d.w., respectively were achieved from MAE of C. odorata leaf at irradiation time of 2 min, microwave power of 600 W, temperature of 60 °C, solvent:feed ratio of 10:1 mL/g, and ethanol concentration of 40% v/v through one-factor-at-time (OFAT) experimental trials. The results obtained from a two-level factorial design experiments reflected that only ethanol concentration (20–60% v/v), irradiation time (1–5 min) and microwave power level (400–800 W) had significant effects on the yields of total phenolic content (TPC) and total flavonoid content (TFC) from C. odorata leaves (p < 0.05). However, temperature and solvent/feed ratio was not significant. In addition, the interactions AB (irradiation time and microwave power) and AE (irradiation time and ethanol concentration) contributed greatly to the recovery yields.

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Soxhlet extraction of phenolic compounds from Vernonia cinerea leaves and its antioxidant activity

Cited by 127 publications

References 16 publications

Optimizing Microwave‐Assisted Extraction Conditions to Obtain Phenolic‐Rich Extract from Chromolaena odorata Leaves

Optimizing Microwave‐Assisted Extraction Conditions to Obtain Phenolic‐Rich Extract from Chromolaena odorata Leaves

Conventional and Emerging Extraction Processes of Flavonoids

Screening of Microwave-Assisted-Batch Extraction Parameters for Recovering Total Phenolic and Flavonoid Contents from <i>Chromolaena odorata</i> Leaves through Two-Level Factorial Design

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