SOX2 in Gastric Carcinoma, but not Hath1, is Related to Patients’ Clinicopathological Features and Prognosis

Zhang, Xin; Yu, Hao; Yang, Yongtao; Zhu, Rong; Bai, Jianying; Peng, Zhihong; He, Yonghong; Chen, Lei; Chen, Wensheng; Fang, Dian‐Chun; Bian, Xiu‐Wu; Wang, Rongquan

doi:10.1007/s11605-010-1246-3

Cited by 60 publications

(67 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The differences in the expression of these pluripotent genes were highly significant. Expression of OCT4 and SOX2 are associated with clinical outcome in various human malignancies including lung (Sholl et al 2010), esophageal (Bass et al 2009), ovarian (Zhang et al 2010a), cervical (Ji and Zheng 2010) and gastric (Zhang et al 2010b) carcinomas. Expression of NANOG has been identified as a component of an embryonic stem (ES) cell signature in various human carcinomas (Santagata et al 2007;Zbinden et al 2010).…”

Section: Discussionmentioning

confidence: 99%

Isolation of colorectal cancer stem-like cells

Dotse

Bian

2014

Cytotechnology

View full text Add to dashboard Cite

This study is aimed at isolating colorectal cancer stem-like cells in vitro using a neurosphere assay method employed in isolating gliobastoma multiforme tumor cells. This was followed with confirmation of the isolated cells by flow cytometry, pluripotent genes expression and in vivo tumorigenicity assay. Using this culture assay, stem-like and non-stem-like CRC cells were isolated and expanded in vitro from purchased Balb/c mice induced with CT26 colorectal cancer (CRC) cell line. The procedure includes an initial mechanical dissociation and chemical digestion of tumor tissue and subsequently plating the resulting single cell suspension in serum-free medium (SFM) or serum-containing medium (SCM). This selectively permits growth of cancer stem-like cells in SFM and eliminates non-stem-like cancer cells through the process of anoikis or apoptosis. CRC stem cells derived cultures proliferated as non-adherent spheres in vitro in different shapes and sizes. These cells expressed cell surface markers previously reported for tumor stem cells, including CD44, CD133, CD166 and CD26 and formed tumors when implanted in severe combined immunodeficient mice in a concentration dependent manner. Importantly, the stem-like cells had self-renewal properties with significantly higher expression of the pluripotent stem cell genes NANOG, OCT4, and SOX2 compared to the adherent non-stem cells. Collectively, the results of this study indicate that SFM is a defined culture medium that enriches for CRC stem-like cells and represents a suitable in vitro model for the study of CRC stem-like cells. This finding may be useful in developing therapeutic strategies aimed at eradicating the tumorigenic subpopulation within colorectal cancer.

show abstract

Section: Discussionmentioning

confidence: 99%

Isolation of colorectal cancer stem-like cells

Dotse

Bian

2014

Cytotechnology

View full text Add to dashboard Cite

show abstract

“…Further interest is in the differential regulation of gastric or intestinal transcription factors. A group from China reported that expression of SOX2 in GC is independent from H. pylori status and that there is a survival benefit for SOX2-positive entities with a negative association to metastases and the clinical stage of disease [56]. In contrast, Sonic Hedgehog (SHH) is upregulated in GC showing a positive association to H. pylori status, even stronger in case of positive CagA status [57].…”

Section: Differential Gene Regulation In Gcmentioning

confidence: 99%

Gastric Cancer: Clinical Aspects, Epidemiology and Molecular Background

et al. 2011

View full text Add to dashboard Cite

In 2010, the WHO introduced the seventh edition of the tumor node metastasis (TNM) classification that provides a more detailed classification for both local tumor invasion (T) and lymph node involvement (N) compared with the previous version. The T and N categories of tumors located in the stomach have been further modified with the intention to ensure a better correlation to the prognostic outcome. For the classification of a pN0, the number of lymph nodes has been adjusted to 16.The description of tumors with origin in the esophagus has been simplified and includes tumors of the esophagogastric junction as well as gastric tumors extending to the proximal 5 cm of the stomach. Major changes are further the subclassification of the categories T1 and T4 and the new N categorization now considering the number of lymph nodes involved within tree categories. Furthermore, positive lymph nodes in the region of the celiac trunc are considered as regional in case of esophageal cancers.The validity of the new classification system in its prognostic efficacy was compared to the previous edition. A study conducted in China proved a better prognostic stratification for the new actualized version [1]. Another study from Korea showed similar results for the new TNM system, proving a more detailed prognostic assessment, especially between T2 and T3 and N1 and N2 tumors [2]. The advantage of the new (7th) edition was mainly confirmed for the prognostic value of accurate lymph node assessment [3].Concerning the classification of early gastric cancer (GC) in the new system, a study from Italy confirmed the usefulness of the new classification for metastatic lymph nodes as a prognostic tool in case of early GC. AbstractThe validity and usefulness of the 7th edition of the UICC tumor node metastasis classification in the context of clinical management of gastric cancer are discussed. The most relevant new agent in gastric cancer therapy is trastuzumab for HER2-positive gastric carcinomas. This marks the success of continuous effort of translational research. Trastuzumab, initially applied in palliative settings, is currently being evaluated also in neoadjuvant treatment regimens. Several new meta-analyses support the carcinogenic effect of high salt intake and smoking in the context of Helicobacter pylori infection. Further data have become available on the efficacy of protective agents, acetyl salicylic acid ⁄ nonsteroidal anti-inflammatory drugs, and antioxidants. In search for a successful prevention strategy, the focus is on the identification of individuals at high risk who demand screening (testing) and surveillance. Serological assessment of gastric mucosal abnormalities with increased risk for gastric cancer development is extensively studied, and new data are presented from Asia as well as from Europe. New high-throughput techniques combined with bioinformatic vector analysis open the gate to the identification of new potential diagnostic and therapeutic targets. Furthermore, these approaches allow us to elucidate the int...

show abstract

“…24 These data suggest that expression of SOX2 drives oncogenesis and therefore overexpression in tumors may be associated with a more aggressive tumor phenotype and poorer clinical outcome. However, in lung cancers, contradictory results have been reported so far: while one smaller study suggested that higher SOX2 expression is associated with better outcome in patients suffering from lung squamous cell carcinomas, 25,26 data from a third group present SOX2 expression to be a marker of poor prognosis in stage I lung adenocarcinomas. 19 Together, these findings support the notion that SOX2 upregulation has a major impact on lung cancer initiation and progression.…”

mentioning

confidence: 98%

SOX2 gene amplification and protein overexpression are associated with better outcome in squamous cell lung cancer

Wilbertz¹,

Wagner

Petersen³

et al. 2011

Modern Pathology

167

155

View full text Add to dashboard Cite

The transcription factor SOX2 (3q26.3-q27) is a key regulator of foregut development and an embryonic stem cell factor cooperating during induction of pluripotency in terminally differentiated somatic cells. Recently, we found SOX2 to be amplified in a subset of squamous cell lung and esophageal cancers. The aim of this study was to explore the prognostic role of SOX2 in a large series of squamous cell carcinomas and adenocarcinomas of the lung. A total of 891 samples from two independent population-based cohorts were assessed by fluorescence in situ hybridization and immunohistochemistry. Furthermore, we assessed for associations between SOX2 amplification/upregulation and clinicopathological features. Similar results were found in the two cohorts. Within squamous cell carcinoma cases, 8% high-level as well as 68 and 65% low-level SOX2 amplifications occurred in the two cohorts, respectively. In adenocarcinomas, no high-level amplification was found and low-level amplification occurred in 6% of the two cohorts. Within squamous cell carcinomas of one cohort, SOX2 amplification was associated with lower tumor grade, while higher levels of SOX2 expression were related to younger age, smaller tumor size, and lower probability of angiolymphatic invasion and metastasis. High SOX2 expression levels proved to be a marker for prolonged overall survival among patients with squamous cell carcinomas. In conclusion, SOX2 amplification and upregulation are frequent events in squamous cell carcinomas of the lung and are associated with indicators of favorable prognosis. Modern Pathology (2011) 24, 944-953;

show abstract

SOX2 in Gastric Carcinoma, but not Hath1, is Related to Patients’ Clinicopathological Features and Prognosis

Abstract: SOX2 and Hath1 are inversely expressed in gastric carcinoma. SOX2 provides a survival advantage to patients of gastric carcinoma and appears to be associated with metastasis and clinical stages.

Cited by 60 publications

References 19 publications

Isolation of colorectal cancer stem-like cells

Isolation of colorectal cancer stem-like cells

Gastric Cancer: Clinical Aspects, Epidemiology and Molecular Background

SOX2 gene amplification and protein overexpression are associated with better outcome in squamous cell lung cancer

Contact Info

Product

Resources

About